Photosynthetic Activity Measured In Situ in Microalgae Cultures Grown in Pilot-Scale Raceway Ponds.

The microalga sp. (Chlorophyceae) was cultured in a raceway pond (RWP) placed in a greenhouse. The objective of this case study was to monitor the photosynthesis performance and selected physicochemical variables (irradiance, temperature, dissolved oxygen concentration) of microalgae cultures in situ at various depths of RWP.

Thermochemical valorization of greenhouse cucumber, tomato and pepper as biofuel.

Agricultural residues adequate managing contributes to reduce CO emissions and easy the circular economy. In this paper, cucumber, tomato and pepper greenhouse crop residues were characterized both chemically and energetically to potential of waste as a biofuel. When compared to other herbaceous and woody biomass, the data show higher moisture, ash and chlorine contents.

Identification of Marine Biotechnology Value Chains with High Potential in the Northern Mediterranean Region.

Marine (blue) biotechnology is an emerging field enabling the valorization of new products and processes with massive potential for innovation and economic growth. In the Mediterranean region, this innovation potential is not exploited as well as in other European regions due to a lack of a clear identification of the different value chains and the high fragmentation of business innovation initiatives. As a result, several opportunities to create an innovative society are being missed.

Photosynthetic performance of Chlamydopodium (Chlorophyta) cultures grown in outdoor bioreactors.

The microalga Chlamydopodium fusiforme MACC-430 was cultured in two types of outdoor pilot cultivation units-a thin-layer cascade (TLC) and a raceway pond (RWP) placed in a greenhouse. This case study aimed to test their potential suitability for cultivation scale-up to produce biomass for agriculture purposes (e.g.

Impact of photobioreactor design on microalgae-bacteria communities grown on wastewater: Differences between thin-layer cascade and thin-layer raceway ponds.

Thin-layer (TL) photobioreactors (PBRs) are characterised by high productivity. However, their use is limited to lab/pilot-scale, and a deeper level of characterisation is needed to reach industrial scale and test the resistance of multiple microalgae. Here, the performance and composition of eight microalgal communities cultivated in the two main TLs design (thin-layer cascade (TLC) and thin-layer raceway pond (RW)) were investigated through Illumina sequencing.

Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors.

Microalgae cultures were used for a WW treatment to remediate nutrients while producing biomass and recycling water. In these trials, raceway ponds (RWPs; 1 and 0.5 ha) were located next to a municipal (WW) treatment plant in Mérida, Spain.

Simulation and Techno-Economical Evaluation of a Microalgal Biofertilizer Production Process.

Due to population growth in the coming years, an increase in agricultural production will soon be mandatory, thus requiring fertilizers that are more environmentally sustainable than the currently most-consumed fertilizers since these are important contributors to climate change and water pollution. The objective of this work is the techno-economic evaluation of the production of biofertilizer concentrated in free amino acids from microalgal biomass produced in a wastewater treatment plant, to determine its economic viability. A process proposal has been made in six stages that have been modelled and simulated with the ASPEN Plus simulator.

Comparative life cycle assessment of conventional and novel microalgae production systems and environmental impact mitigation in urban-industrial symbiosis.

The versatility of microalgae biomass as candidates for various products and bioremediation needs motivates interests towards design and implementation of novel microalgae bioreactors. Conventional open-reactors are reliant on large quantities of sunlight and space while yields are constrained by outdoor environment conditions. Conversely, closed-reactor systems like bubble columns reduces these constrains on microalgae growth while occupying far less space at the expense of high energy demands, notably from lighting systems.

Profiling microalgal cultures growing on municipal wastewater and fertilizer media in raceway photobioreactors.

Microalgae cultivation is proposed as an effective system for pathogens reduction and wastewater depuration, however, a full characterisation of the risks is still needed. Two raceways were inoculated with Scenedesmus, one using wastewater and the other using a fertilizer medium. Microbial community and pathogen presence were explored by next generation sequencing (NGS), commercial qPCR array and plate counts.

Pathogens and predators impacting commercial production of microalgae and cyanobacteria.

Production of phytoplankton (microalgae and cyanobacteria) in commercial raceway ponds and other systems is adversely impacted by phytoplankton pathogens, including bacteria, fungi and viruses. In addition, cultures are susceptible to productivity loss, or crash, through grazing by contaminating zooplankton such as protozoa, rotifers and copepods. Productivity loss and product contamination are also caused by otherwise innocuous invading phytoplankton that consume resources in competition with the species being cultured.

Microalgae based wastewater treatment coupled to the production of high value agricultural products: Current needs and challenges.

One of the main social and economic challenges of the 21st century will be to overcome the worlds' water deficit expected by the end of this decade. Microalgae based wastewater treatment has been suggested as a strategy to recover nutrients from wastewater while simultaneously producing clean water. Consortia of microalgae and bacteria are responsible for recovering nutrients from wastewater.

Effect of operational parameters, environmental conditions, and biotic interactions on bacterial communities present in urban wastewater treatment photobioreactors.

The effects of water depth, operational and environmental conditions on bacterial communities were analyzed in microalgal-bacterial outdoor photobioreactors treating urban wastewaters from March to August 2014. Three raceway photobioreactors inoculated with Scenedesmus sp. and with different water depths (20, 12, and 5 cm) were used at different dilution rates (0.

OPTIMIZATION OF A NEW CULTURE MEDIUM FOR THE LARGE-SCALE PRODUCTION OF PROTEIN-RICH ARTHROSPIRA PLATENSIS (OSCILLATORIALES, CYANOPHYCEAE).

Our aim was to develop a novel medium for the large-scale production of protein-rich Arthrospira with potential applications as a biofertilizer. The novel culture medium, termed as FM-II, was formulated using low-cost commercial chemicals and specifically designed to improve protein production. Both Arthrospira platensis and Arthrospira maxima were produced using FM-II and Arnon medium, which was used as a control.

Influence of photobioreactor set-up on the survival of microalgae inoculum.

Cultivation of specific microalgae is still difficult in an industrial setup as contamination and balancing the economic cost are not always possible. Understanding the ecology of cultivation of microalgae is therefore necessary to implement stable production. The aim of the study was to understand how different types of photobioreactors and types of culture medium influenced the survival of a specific microalgae inoculum, S.

Spirulina for the food and functional food industries.

Humans are no strangers to the consumption of microalgae as already in the sixteenth century Spirulina was harvested from Lake Texcoco and consumed in markets in Tenochtitlan (today Mexico City). Nowadays, microalgae are being incorporated into many food formulations. Most of these use microalgae as a marketing strategy or as a colouring agent.

Utilization of centrate for the outdoor production of marine microalgae at the pilot-scale in raceway photobioreactors.

In this study, the outdoor production of marine microalgae in raceway photobioreactors was investigated, modifying the centrate percentage in the culture medium (20, 30, 40 and 50%) and using two different dilution rates (0.2 day and 0.3 day).

A new approach for detection and quantification of microalgae in industrial-scale microalgal cultures.

In industrial-scale microalgal cultures, non-target microalgae compete with the desired species for nutrients and CO, thus reducing the growth rate of the target species and the quality of the produced biomass. Microalgae identification is generally considered a complicated issue; although, in the last few years, new molecular methods have helped to rectify this problem. Among the different techniques available, DNA barcoding has proven very useful in providing rapid, accurate, and automatable species identification; in this work, it is used to assess the genomic identity of the microalga species Scenedesmus sp.

A whole biodiesel conversion process combining isolation, cultivation and in situ supercritical methanol transesterification of native microalgae.

A coupled process combining microalgae production with direct supercritical biodiesel conversion using a reduced number of operating steps is proposed in this work. Two newly isolated native microalgae strains, identified as Chlorella sp. and Nannochloris sp.

New approach to solar photo-Fenton operation. Raceway ponds as tertiary treatment technology.

The photo-Fenton process has proven its efficiency in the removal of micropollutants. However, the high costs usually associated with it prevent a spread of this technology. An important factor affecting costs is the kind of photoreactor used, usually tubular with a reflecting surface.

Protein measurements of microalgal and cyanobacterial biomass.

The protein content of dry biomass of the microalgae Porphyridium cruentum, Scenedesmus almeriensis, and Muriellopsis sp. and of the cyanobacteria Synechocystis aquatilis and Arthrospira platensis was measured by the Lowry method following disruption of the cells by milling with inert ceramic particles. The measurements were compared with the Kjeldahl method and by elemental analysis.