The use of abrasive polishing and laser processing for developing polyurethane surfaces for controlling fibroblast cell behaviour.

Studies have shown that surfaces having micro and nano-scale features can be used to control cell behaviours including; cell proliferation, migration and adhesion. The aim of this work was to compare the use of laser processing and abrasive polishing to develop micro/nano-patterned polyurethane substrates for controlling fibroblast cell adhesion, migration and proliferation. Laser processing in a directional manner resulted in polyurethane surfaces having a ploughed field effect with micron-scale features.

The effects of acoustic vibration on fibroblast cell migration.

Cells are known to interact and respond to external mechanical cues and recent work has shown that application of mechanical stimulation, delivered via acoustic vibration, can be used to control complex cell behaviours. Fibroblast cells are known to respond to physical cues generated in the extracellular matrix and it is thought that such cues are important regulators of the wound healing process. Many conditions are associated with poor wound healing, so there is need for treatments/interventions, which can help accelerate the wound healing process.

Aiding phase unwrapping by increasing the number of residues in two-dimensional wrapped-phase distributions.

In phase unwrapping residues are points of locally inconsistent phase that occur within a wrapped-phase map, which are usually regarded as being problematic for phase-unwrapping algorithms. Real phase maps typically contain a number of residues that are approximately proportional to the subsequent difficulty in unwrapping the phase distribution. This paper suggests the radical use of the discrete Fourier transform to actually increase the number of residues in 2D phase-wrapped images that contain discontinuities.

Analysis of dynamic cantilever behavior in tapping mode atomic force microscopy.

Tapping mode atomic force microscopy (AFM) provides phase images in addition to height and amplitude images. Although the behavior of tapping mode AFM has been investigated using mathematical modeling, comprehensive understanding of the behavior of tapping mode AFM still poses a significant challenge to the AFM community, involving issues such as the correct interpretation of the phase images. In this paper, the cantilever's dynamic behavior in tapping mode AFM is studied through a three dimensional finite element method.

Absolute distance measurement with micrometer accuracy using a Michelson interferometer and the iterative synthetic wavelength principle.

We present a novel system that can measure absolute distances of up to 300 mm with an uncertainty of the order of one micrometer, within a timeframe of 40 seconds. The proposed system uses a Michelson interferometer, a tunable laser, a wavelength meter and a computer for analysis. The principle of synthetic wave creation is used in a novel way in that the system employs an initial low precision estimate of the distance, obtained using a triangulation, or time-of-flight, laser system, or similar, and then iterates through a sequence of progressively smaller synthetic wavelengths until it reaches micrometer uncertainties in the determination of the distance.

Actin bundling and polymerisation properties of eukaryotic elongation factor 1 alpha (eEF1A), histone H2A-H2B and lysozyme in vitro.

Elongation factor 1 alpha (eEF1A) is a positively charged protein which has been shown to interact with the actin cytoskeleton. However, to date, a specific actin binding site within the eEF1A sequence has not been identified and the mechanism by which eEF1A interacts with actin remains unresolved. Many protein-protein interactions occur as a consequence of their physicochemical properties and actin bundle formation has been shown to result from non-specific electrostatic interaction with basic proteins.

Evaluation and benchmarking of a pixel-shifting camera for superresolution lensless digital holography.

In lensless digital holography, the comparatively low resolution of the CCD devices that are used to record the digital holograms has to date limited both the maximum linear dimensions of the measurement object and also the minimum possible stand-off distance between the object and the CCD detector. A signal-processing-based technique known as superresolution (SR) image reconstruction can provide an alternative approach that reduces these restrictions. We report on an SR image reconstruction technique that has been introduced by employing a camera with a "microscanning" function to capture SR digital holograms via multiple subpixel movements of the CCD sensor.

Dynamic recalibration of scalable fringe-projection systems for large-scale object metrology.

Three-dimensional (3D) surface shape measurement is a vital component in many industrial processes. The subject has developed significantly over recent years and a number of mainly noncontact techniques now exist for surface measurement, exhibiting varying levels of maturity. Within the larger group of 3D measurement techniques, one of the most promising approaches is provided by those methods that are based upon fringe analysis.

Three-dimensional phase unwrapping using the Hungarian algorithm.

We propose a three-dimensional phase unwrapping technique that uses the Hungarian algorithm to join together all the partial residual loops that may occur in a wrapped phase volume. Experimental results have shown that the proposed algorithm is more robust and reliable than other well-known three-dimensional phase unwrapping algorithms. Additionally, the proposed algorithm is fast in terms of computational complexity, which makes it suitable for practical applications.

Robust three-dimensional best-path phase-unwrapping algorithm that avoids singularity loops.

In this paper we propose a novel hybrid three-dimensional phase-unwrapping algorithm, which we refer to here as the three-dimensional best-path avoiding singularity loops (3DBPASL) algorithm. This algorithm combines the advantages and avoids the drawbacks of two well-known 3D phase-unwrapping algorithms, namely, the 3D phase-unwrapping noise-immune technique and the 3D phase-unwrapping best-path technique. The hybrid technique presented here is more robust than its predecessors since it not only follows a discrete unwrapping path depending on a 3D quality map, but it also avoids any singularity loops that may occur in the unwrapping path.

Fast and robust three-dimensional best path phase unwrapping algorithm.

What we believe to be a novel three-dimensional (3D) phase unwrapping algorithm is proposed to unwrap 3D wrapped-phase volumes. It depends on a quality map to unwrap the most reliable voxels first and the least reliable voxels last. The technique follows a discrete unwrapping path to perform the unwrapping process.

Spatial fringe pattern analysis using the two-dimensional continuous wavelet transform employing a cost function.

We present a novel ridge extraction algorithm for use with the two-dimensional continuous wavelet transform to extract the phase information from a fringe pattern. A cost function is employed for the detection of the ridge. The results of the proposed algorithm on simulated and real fringe patterns are illustrated.

Comparative study of the conditions required to image live human epithelial and fibroblast cells using atomic force microscopy.

Successful imaging of living human cells using atomic force microscopy (AFM) is influenced by many variables including cell culture conditions, cell morphology, surface topography, scan parameters, and cantilever choice. In this study, these variables were investigated while imaging two morphologically distinct human cell lines, namely LL24 (fibroblasts) and NCI H727 (epithelial) cells. The cell types used in this study were found to require different parameter settings to produce images showing the greatest detail.

Quantization error of CCD cameras and their influence on phase calculation in fringe pattern analysis.

We present an investigation into the phase errors that occur in fringe pattern analysis that are caused by quantization effects. When acquisition devices with a limited value of camera bit depth are used, there are a limited number of quantization levels available to record the signal. This may adversely affect the recorded signal and adds a potential source of instrumental error to the measurement system.

Opto-electronic sensing of body surface topology changes during radiotherapy for rectal cancer.

Purpose: The CT body surface underpins millimeter scale dose computation in radical radiotherapy. A lack of technology has prevented measurement of surface topology changes during irradiation. Consequently, body changes are incorporated into plans statistically.