Publications by authors named "Francis A Kallfelz"
Objective: To perform respiratory chain enzymatic activity assays on canine skeletal muscle biopsy specimens and establish reference range values of skeletal muscle enzyme activities for dogs.
Sample Population: Biopsy specimens from the vastus lateralis muscle were obtained from 24 dogs (8 sexually intact males and 14 sexually intact females) ranging from 15 months to 6 years of age.
Procedure: Mean values of citrate synthase, cytochrome-c oxidase, succinate dehydrogenase, succinate dehydrogenase-cytochrome-c reductase, nicotinamide adenine dinucleotide (NADH) dehydrogenase, and NADH dehydrogenase-cytochrome-c reductase activities were established by use of 6 standard spectrophotometric assays for respiratory chain enzyme analysis.
Post-exercise carbohydrate supplementation has been routinely used to enhance glycogen concentrations in skeletal muscle, particularly during multiple-day athletic events. Consumption of protein hydrolysates mixed with carbohydrate supplements during the post-exercise period may increase insulin response and cause glycogen repletion in skeletal muscle. A group of Alaskan sled dogs were selected to examine post-exercise supplementation in a paired crossover study design.
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- Long-term athletic training in dogs, specifically English pointers and Labrador retrievers, leads to changes in muscle protein turnover, particularly concerning the ubiquitin-proteasome (UP) pathway, which is connected to muscle breakdown and repair.
- A study examined 44 hunting dogs by collecting muscle biopsies before and during peak activity seasons to analyze the activity levels of the UP pathway and other muscle-related factors.
- Results showed an increase in certain components of the UP pathway and muscle glycogen levels during peak activity, suggesting that these biological changes contribute to muscle adaptation in response to rigorous athletic training.