Publications by authors named "Francesco Spedicato"
Article Synopsis
- This study investigates how N-methylpyridinium (NMP), an alkaloid found in coffee, affects lipid metabolism in liver cells influenced by Type 2 diabetes and nonalcoholic fatty liver diseases (NAFLDs).
- NMP was tested in a diabetes cell model, showing that it reduces lipid droplet accumulation and regulates gene expression related to lipogenesis by improving cellular oxidative status and endoplasmic reticulum (ER) stress response.
- The findings indicate that NMP could be beneficial for managing Type 2 diabetes and NAFLD by enhancing lipid metabolism and restoring cellular homeostasis.
Article Synopsis
- CACT is an important protein that helps transport long-chain fatty acids into mitochondria for energy production, and this study focuses on how its gene regulation is affected by omega-3 fatty acids (EPA and DHA).
- In liver cells, EPA and DHA were found to increase the levels of CACT mRNA and protein, highlighting their role in promoting this gene's expression.
- The research identified a specific region in the CACT promoter that responds to n-3 PUFAs, involving GABP proteins rather than PPARα, suggesting that GABP may play a key role in the activation of the CACT gene by these fatty acids.
Article Synopsis
- N-methylpyridinium (NMP) is created during coffee roasting from trigonelline and has potential health benefits due to its antioxidant properties.
- Preliminary studies have shown that NMP may protect against LPS-induced neuroinflammation in human glioblastoma cells by reducing pro-inflammatory cytokines and inhibiting the NF-κB signaling pathway.
- This research suggests that drinking coffee, which contains NMP, could help protect against neuroinflammatory issues relevant to neurological disorders.
The goal of regenerative medicine is to achieve tissue regeneration. In the past, commonly used techniques included autologous or allogeneic transplantation and stem cell therapy, which have limitations, such as a lack of donor sites in the case of autologous transplantation and the invasiveness of stem cell harvesting. In recent years, research has, therefore, focused on new and less invasive strategies to achieve tissue regeneration.
View Article and Find Full Text PDFThe application of scaffolding materials together with stem cell technologies plays a key role in tissue regeneration. Therefore, in this study, CGF (concentrated growth factor), which represents an autologous and biocompatible blood-derived product rich in growth factors and multipotent stem cells, was used together with a hydroxyapatite and silicon (HA-Si) scaffold, which represents a very interesting material in the field of bone reconstructive surgery. The aim of this work was to evaluate the potential osteogenic differentiation of CGF primary cells induced by HA-Si scaffolds.
View Article and Find Full Text PDFThe heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is the most abundant and ubiquitously expressed member of the heterogeneous nuclear ribonucleoproteins family (hnRNPs). hnRNP A1 is an RNA-binding protein associated with complexes active in diverse biological processes such as RNA splicing, transactivation of gene expression, and modulation of protein translation. It is overexpressed in several cancers, where it actively promotes the expression and translation of several key proteins and regulators associated with tumorigenesis and cancer progression.
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- * Previous studies suggested that marine sulfate-rich waters travel deep underground, warming and interacting with organic materials to produce hydrogen sulfide through sulfate reduction, but no detailed studies on the origin of hydrogen sulfide in this specific site have been done.
- * A new study employs a multidisciplinary approach to analyze the chemistry and microbial diversity in Zinzulùsa cave, revealing that sulfate from marine waters and specific sulfate-reducing bacteria contribute to hydrogen sulfide production, while proposing a chemotrophic model that utilizes hydrocarbon deposits as energy
Additional chromosome abnormalities (ACAs) occur in less than 10% of cases at diagnosis of Philadelphia chromosome (Ph)-positive chronic myelogenous leukemia (CML). In some cases, on the basis of the persistence of the ACAs in Ph-negative cells after response to imatinib, a secondary origin of the Ph chromosome has been demonstrated. In this study, the possible prognostic value of this phenomenon was evaluated.
View Article and Find Full Text PDFPersistence of chromosomal abnormalities additional to the Philadelphia chromosome after Philadelphia chromosome disappearance during imatinib therapy for chronic myeloid leukemia.
Haematologica
April 2007
Five Philadelphia chromosome positive (Ph+) chronic myeloid leukemia (CML) patients with additional chromosome abnormalities at diagnosis have been followed during Imatinib therapy. In all, the Ph chromosome disappeared, while the 5 cases, additional abnormalities [dup(1); del(5), +8 (2 patients) and +14] persisted in the subsequent studies, performed over a period of 11 to 49 months, either alone or together with a karyotypically normal cell population. This finding is consistent with a secondary origin of the Ph chromosome in these patients.
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