Publications by authors named "Francesco Alfani"
Steam-exploded (SE) poplar wood biomass was hydrolyzed by means of a blend of Celluclast and Novozym cellulase complexes in the presence of the inhibiting compounds produced during the preceding steam-explosion pretreatment process. The SE temperature and time conditions were 214 degrees C and 6 min, resulting in a log R(0) of 4.13.
View Article and Find Full Text PDFArticle Synopsis
- A new experimental technique using a continuous stirred-tank reactor is introduced for examining enzyme kinetics when substrates are contaminated with inhibitors.
- The method is mathematically simulated to analyze various inhibition types: competitive, uncompetitive, and mixed-type noncompetitive.
- The study includes a detailed experimental procedure and data analysis approach for identifying kinetic parameters, alongside illustrations of how enzyme inhibition differs based on excess substrate versus impurities.