Publications by authors named "Francescantonio D'Orilia"
West Nile virus (WNV) is the most widespread arbovirus worldwide, responsible for severe neurological symptoms in humans as well as in horses and birds. The main reservoir and amplifier of the virus are birds, and migratory birds seem to have a key role in the introduction and spread of WNV during their migratory routes. WNV lineage 1 (L1) has been missing in Italy for almost 10 years, only to reappear in 2020 in two dead raptor birds in southern Italy.
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- * This study validated a sensitive digital droplet polymerase chain reaction (ddPCR) method to detect and quantify Toxoplasma DNA in mussels, demonstrating its effectiveness with a 100% sensitivity and specificity.
- * In testing 100 mussel samples from the Gulf of Naples, 16% were found positive for Toxoplasma, showing that ddPCR outperforms traditional methods like real-time PCR in detecting low DNA levels, thereby reducing Toxoplasmosis risk in humans.