Cell counting and velocity algorithms for hydrodynamic study of unsteady biological flows in micro-channels.

In this paper, the combination of two algorithms, a cell counting algorithm and a velocity algorithm based on a Digital Particle Image Velocimetry (DPIV) method, is presented to study the collective behavior of micro-particles in response to hydrodynamic stimuli. A wide experimental campaign was conducted using micro-particles of different natures and diameters (from to ), such as living cells and silica beads. The biological fluids were injected at the inlet of a micro-channel with an external oscillating flow, and the process was monitored in an investigated area, simultaneously, through a CCD camera and a photo-detector.