Site-specific N- and O-glycosylation analysis of atacicept.

The Fc-fusion protein atacicept is currently under clinical investigation for its biotherapeutic application in autoimmune diseases owing to its ability to bind the two cytokines B-Lymphocyte Stimulator (BLyS) and A PRoliferation-Inducing Ligand (APRIL). Like typical recombinant IgG-based therapeutics, atacicept is a glycoprotein whose glycosylation-related heterogeneity arises from the glycosylation-site localization, site-specific occupation and structural diversity of the attached glycans. Here, we present a first comprehensive site-specific N- and O-glycosylation characterization of atacicept using mass spectrometry-based workflows.

In-vivo biological activity and glycosylation analysis of a biosimilar recombinant human follicle-stimulating hormone product (Bemfola) compared with its reference medicinal product (GONAL-f).

Recombinant human follicle-stimulating hormone (r-hFSH) is widely used in fertility treatment. Although biosimilar versions of r-hFSH (follitropin alfa) are currently on the market, given their structural complexity and manufacturing process, it is important to thoroughly evaluate them in comparison with the reference product. This evaluation should focus on how they differ (e.

Analysis of native and APTS-labeled N-glycans by capillary electrophoresis/time-of-flight mass spectrometry.

The glycosylation of proteins is of particular interest in biopharmaceutical applications. The detailed characterization of glycosylation based on the released carbohydrates is mandatory since the protein stability, folding, and efficacy are strongly dependent on the structural diversity inherent in the glycan moieties of a glycoprotein. For glycan pattern analysis, capillary electrophoresis with laser-induced fluorescence using 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled glycans is used frequently.

Antioxidant and radical scavenging properties of Malva sylvestris.

Antioxidant capacity of the aqueous extract of Malva sylvestris was measured by its ability to scavenge the DPPH and superoxide anion radicals and to induce the formation of a phosphomolybdenum complex. Analysis of the extract, carried out by different chromatographic techniques, led to the isolation of eleven compounds: 4-hydroxybenzoic acid, 4-methoxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 4-hydroxycinnamic acid, ferulic acid, methyl 2-hydroxydihydrocinnamate, scopoletin, N-trans-feruloyl tyramine, a sesquiterpene, (3R,7E)-3-hydroxy-5,7-megastigmadien-9-one, and (10E,15Z)-9,12,13-trihydroxyoctadeca-10,15-dienoic acid. The antioxidant activities of all these compounds are reported.

Chenoalbicin, a novel cinnamic acid amide alkaloid from Chenopodium album.

The roots of Chenopodium album were infused in MeOH, and the extract was partitioned between AcOEt and H2O. AcOEt-Soluble material was subjected to different silica-gel column chromatographies and then purified by reverse-phase HPLC to afford a new cinnamic acid amide alkaloid as a racemic mixture. The new compound, named chenoalbicin (1), was characterized by extensive spectroscopic investigation, especially 1D and 2D NMR spectroscopy.

Phenols and lignans from Chenopodium album.

Cinnamic acid, 4-hydroxy-cinnamic acid, ferulic acid, methyl ferulate, sinapic acid, methyl 3-(4-hydroxy-3-methoxyphenyl)propanoate, 4-(1-hydroxyethyl)-2-methoxyphenol, vanillyl alcohol, 4-(hydroxymethyl)-2-methoxyphenol, 4-hydroxy-3-methoxybenzoic acid, 4-vinylphenol, 4-methylbenzaldehyde, N-[2-(1H-indol-3-yl)ethyl]acetamide, pinoresinol, syringaresinol, lariciresinol, 5,5'-dimethoxy-lariciresinol, threo-guaiacylglycerol-3-beta-4-syringaresinol ether and two new sesquilignans, namely, threo-guaiacylglycerol-alpha-O-methyl-beta-O-4-syringaresinol ether and threo-syringylglycerol-alpha-O-methyl-beta-O-4-syringaresinol ether, were isolated and identified as components of Chenopodium album. Constitutions were established on the basis of spectroscopic data, including two-dimensional NMR analyses.

Terpenoids and phenol derivatives from Malva silvestris.

A sesquiterpene and a tetrahydroxylated acyclic diterpene as well as two known monoterpenes, 6 C(13)nor-terpenes and 11 aromatic compounds were isolated from the water extract of Malva silvestris. The structures of the compounds were determined by spectroscopic NMR and MS analysis. Effects of these compounds on germination and growth of dicotyledon Lactuca sativa L.

C13 norisoprenoids from Brassica fruticulosa.

Five C13 norisoprenoids, one of them glucosilated, have been isolated from the leaves of Brassica fruticulosa (Brassicaceae). The structure (3S,4R,7E,9S)-3,4,9-trihydroxy-5,7-megastigmadiene, has been assigned to the new compound. All the structures have been determined by spectroscopic means and chemical correlations.

Cinnamic acid amides from Chenopodium album: effects on seeds germination and plant growth.

Seven cinnamic acid amides have been isolated from Chenopodium album. The structures have been attributed by means of their spectral data. One of them, N-trans-4-O-methylferuloyl 4'-O-methyldopamine, is described for the first time.

Lignans and neolignans from Brassica fruticulosa: effects on seed germination and plant growth.

Five lignans, five neolignans, two sesquilignans, and a dilignan were identified from a phytotoxic extract of Brassica fruticulosa L. Compounds 8, 9, 12, and 13 have been isolated for the first time. Structures were determined on the basis of their spectroscopic features.