Publications by authors named "Francesca Canyelles I Font"

Article Synopsis
  • Bioluminescent high-throughput screening (HTS) assays using firefly, Renilla, and NanoLuc luciferases are essential tools in research and drug discovery, but can be affected by various metal ions present in biological and environmental samples.
  • A study screened 21 metal ions in three different HTS assays, quantifying their interference with luminescence signals and developing optimized buffer variants for better luciferase performance.
  • Findings showed that metal ions significantly impacted bioluminescence signals in a way that correlates with known metal ion-ligand affinities, indicating complex interactions that vary depending on the type of luciferase and assay conditions used.
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Luminescence has found wide application in biology, biotechnology and medicine. Particularly, fluorescent and bioluminescent probes allow visualization of molecular targets at the cellular level and even macromolecules or single small-molecule analytes. Among the most reliable tools for visualization of molecular targets are so-called responsive probes, which change the intensity and colour of the emitted signal after interaction with a molecular target.

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