Publications by authors named "Francesca Buzzolini"
Anti-double-stranded DNA antibodies (anti-dsDNA) are considered a specific marker for systemic lupus erythematosus (SLE). Though the Farr technique was once the reference method for their detection, it has been almost entirely replaced by more recently developed assays. However, there is still no solid evidence of the commutability of these methods in terms of diagnostic accuracy and their correlation with the Crithidia luciliae immunofluorescence test (CLIFT).
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- Autoantibodies against extractable nuclear antigens (ENA) are essential for diagnosing systemic autoimmune rheumatic diseases (SARD), and new methods allow for simultaneous detection of multiple anti-ENA reactivities.
- The study compared eight different immunoassays on sera from 60 SARD patients, 10 inflammatory arthritis patients, and 10 healthy donors to assess their sensitivity, specificity, and diagnostic accuracy.
- Results showed good agreement among methods (average kappa of 0.82) and high specificity, but there were notable differences in analytical sensitivity, highlighting the need for clinicians to understand the diagnostic capabilities of the assays used.