Publications by authors named "Francesca B Lopez"
Article Synopsis
- The 45S rRNA genes are large repetitive elements in eukaryotic genomes, with many remaining transcriptionally inactive, possibly serving as back-up copies for ribosome production and nuclear organization.
- Using Cas9 genome editing in Arabidopsis thaliana, researchers successfully reduced 45S rDNA copy numbers by up to 90% while maintaining normal rRNA transcription levels, indicating a gene dosage compensation mechanism at play.
- Although the overall genome integrity was preserved, one line exhibited a chromosome segmental duplication, and transcriptome analysis revealed shared dysregulated genes and pathways across independent low copy number lines, highlighting the implications of reduced rDNA copy number on cellular processes and development.
