Comparison of reverse transcriptase PCR, reverse transcriptase loop-mediated isothermal amplification, and culture-based assays for Salmonella detection from pork processing environments.

Novel rapid Salmonella detection assays without the need for sophisticated equipment or labor remain in high demand. Real-time reverse transcriptase PCR (RT-PCR) assays, though rapid and sensitive, require expensive thermocyclers, while a novel RT loop-mediated isothermal amplification (RT-LAMP) method requires only a simple water bath. Our objective was to compare the detection sensitivity of Salmonella Typhimurium from the pork processing environment by RT-LAMP, RT-PCR, and culture-based assays.

Loop-mediated isothermal amplification (LAMP) for the rapid and sensitive detection of Salmonella Typhimurium from pork.

Unlabelled: Loop-mediated isothermal amplification (LAMP) is a novel molecular detection method that is more rapid and simpler than PCR. Products can be detected by turbidity using one temperature without the need for expensive PCR equipment. Our objective was to sensitively detect Salmonella Typhimurium from pork products within 1 d using the LAMP assay.

Real-time reverse transcriptase PCR for the rapid and sensitive detection of Salmonella typhimurium from pork.

Reverse transcriptase PCR (RT-PCR) detects the presence of mRNA and has a greater potential for detecting viable pathogens than do DNA-based PCR assays, with improved speed and sensitivity compared with traditional methods. Our objective was to rapidly and sensitively detect Salmonella Typhimurium from pork within two 8-h work shifts using a SYBR Green I real-time RT-PCR (rt-RT-PCR) assay. Pork chop and sausage samples (25 g) were inoculated with 10(8) to 10(0) CFU of Salmonella Typhimurium and stomached in 225 ml of tetrathionate broth.

Real-time reverse-transcriptase--polymerase chain reaction for Salmonella enterica detection from jalapeño and serrano peppers.

Outbreaks of Salmonella linked to fresh produce emphasize the need for rapid detection methods to curb the spread of foodborne pathogens. Reverse-transcriptase-polymerase chain reaction (RT-PCR) detects the presence of mRNA (shorter half-life than DNA), with greater potential of detecting viable pathogens. Real-time RT-PCR eliminates the need for gel electrophoresis and significantly enhances the speed of detection (<1 day) compared with traditional methods (>5 days).

Osmotic dehydration of apple slices with CaCl2 and sucrose limits decay caused by Penicillium expansum, Colletotrichum acutatum, and Botrytis cinerea and does not promote Listeria monocytogenes or total aerobic population growth.

The interaction of Penicillium expansum Link, Colletotrichum acutatum, and Botrytis cinerea Pers.:Fr. with Listeria monocytogenes on osmotically dehydrated apple slices was evaluated.

Degradation of Ochratoxin A by Acinetobacter calcoaceticus.

Microorganisms were screened for their ability to degrade ochratoxin A (OTA). Among test microorganisms, Acinetobacter calcoaceticus was found to degrade OTA. The degradation of OTA by A.

Survival of Campylobacter jejuni in Modified Atmosphere Packaged Turkey Roll.

Survival of Campylobacter jejuni , inoculated into turkey roll slices and stored under seven different atmospheric mixtures, was determined. Turkey roll samples were stored at 4°C for 18 d and at 21°C for 48 h. The effects of various atmospheric mixtures on aerobic, psychrotrophic, and lactic acid bacterial populations were also determined throughout storage.

Infection of Shell Eggs With Yersinia Enterocolitica.

Shell eggs were immersed in bacterial suspensions containing 10 Yersinia enterocolitica / and subjected to either a temperature differential or a pressure differential inoculation technique in the presence or absence of 20 ppm iron to determine if Y. enterocolitica could penetrate and infect shell eggs. No Y.

Growth Characteristics of Yersinia enterocolitica in Pasteurized Skim Milk.

Experiments were undertaken to determine the growth characteristics of five strains of Yersinia enterocolitica in pasteurized milk at 4°C. Pasteurized milk was inoculated with approximately 10 or 1000 cells/ml of Y. enterocolitica , and was incubated at 4°C for 0, 3, 7, 14 and 21 d.

Campylobacter jejuni in Vacuum Packaged Processed Turkey.

This study evaluated the effect of vacuum packaged storage at 4°C upon survival of Campylobacter jejuni in processed turkey roll and turkey ham. Turkey ham and turkey roll samples were sliced, inoculated with C. jejuni , vacuum packaged, and stored at 4°C for up to 28 d.

Chemical and Biological Evaluation of Aflatoxin After Treatment with Sodium Hypochlorite, Sodium Hydroxide and Ammonium Hydroxide.

Aflatoxin B was mixed with eleven concentrations of sodium hydroxide, sodium hypochlorite and ammonium hydroxide. Aflatoxin was quantitated by fluorometric determination and toxicity of aflatoxin treated with NaOH and NHOH was evaluated by the brine shrimp assay. Detoxified aflatoxin B was then screened for mutagenicity using the Salmonella /mammalian microsome mutagenicity test (Ames test).

Inhibition of Patulin Production with the Insecticide Naled.

Patulin is a wide spectrum biocide produced by several species of Aspergillus , Penicillium and Byssochlamys , and is a potentially important mycotoxin that may be ingested by man. The effect of the insecticide naled on fungal growth and patulin production was evaluated using three concentrations (1, 10, and 100 mg/l, ppm) and several application times both before inoculation and during growth of the fungus. If added before inoculation, naled at 100 mg/l completely inhibited mycelial growth of Penicillium urticae for 15 days and patulin production for 30 days.