Publications by authors named "Frackowiak K"

Histidine (His) photo-oxidation has been widely investigated with several transient and stable products characterized, especially for aerobic conditions. Due to its role and structure, His-side chain can be a key player in the quenching of excited states such as the triplet state of the photosensitizer 3-carboxybenzophenone (CB*). The capacity of His and its derivatives to quench CB* under anaerobic conditions are characterized in the current study by laser flash photolysis, with the resulting oxidation products examined by mass spectrometry to determine the reaction mechanism.

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Article Synopsis
  • The study focuses on the complex mechanisms of one-electron protein oxidation, investigating the reaction of sensitized photo-oxidation using carboxybenzophenone (CB) and a specific protein, MtN13.
  • Researchers used crystallography and mass spectrometry to determine how CB interacts with MtN13, identifying specific amino acid residues that form adducts with the sensitizer.
  • The findings reveal new mechanisms for protein oxidation, highlighting the role of ground state complexes and the influence of surrounding amino acids on the oxidation process, particularly in relation to tyrosine.
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Objectives: belongs to enteric parasites responsible for prolonged symptoms in the gastrointestinal tract, both in immunocompetent and immunocompromised individuals. One of the risk factors of infection is contact with an infected person or animals (cattle). The case is described of a young man admitted to the Department of Tropical and Parasitic Diseases of the Medical University in Poznań, Poland, because of watery diarrhea with high fever and in whom symptomatic treatment did not produce any improvement.

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Introduction: Diarrhoea is a common reason for hospitalization among travellers returning from the tropics. The aetiology is predominantly bacterial, but it can also be caused by parasites such as spp., and spp.

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The in-season dynamics of potato tuber biomass (TTB) growth requires effective nitrogen (N) control. This hypothesis was tested in 2006 and 2007. The two-factorial experiment with two rates of N (60, 120 kg ha) and sulfur (S; 0, 50 kg ha) was carried out in the split-plot design.

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Hemin is an activator of heme oxygenase-1 (HO-1), an enzyme catalyzing heme degradation. Up-regulation of HO-1 is observed in response to various pathological conditions. Moreover, pharmacological activation of HO-1 is associated with numerous beneficial effects in the organism.

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Tyrosine residues (Tyr) on proteins are a favoured site of one-electron oxidation due to their low one-electron reduction potentials. In this work, light-induced oxidation of Tyr residues was investigated using direct ionisation (via 266 nm light excitation) and sensitized photo-oxidation (by 3-carboxybenzophenone as sensitizer and 355 nm). Light emission (fluorescence) was observed at 410-440 nm as a result of Tyr oxidation.

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Traveling to tropical countries make very often for travelers a danger of illnesses, which do not exist or exist very rarely in temperate climate. Imported parasitic disease cases are inevitable and have been reported increasingly as a result of enhanced globalization. The most common infections in endemic areas are caused by soil transmitted helminths.

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Protozoa of the genus Cryptosporidium are common parasites of domestic and wild animals-mammals, birds, reptiles, and fishes. The invasive forms are thick-walled oocysts, which can be present in water supplies, on fruits, vegetables, or in the soil contaminated with feces. In this work, we describe three cases of middle-aged persons with massive Cryptosporidium hominis infection and chronic diarrhea with no immunological abnormalities and no history of previous travels to tropical countries.

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Dirofilariasis is a parasitic disease of dogs and other carnivores transmitted mainly by the mosquitoes of the genera Culex, Aedes, Anopheles. Full life cycle of the Dirofilaria nematodes in humans is extremely rarely observed, usually lacking species determination at the molecular level. We report fully documented unusual clinical manifestation of subcutaneous dirofilariasis with intensive microfilariemia in peripheral blood revealed by the Knott's concentration technique.

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Intestinal coccidian parasites are intracellular protozoa most frequently transmitted during food-borne and water-borne infections. This group of parasites is responsible for acute diarrhoeal illnesses especially among immunocompromised patients. However, they are more frequently detected in immunocompetent individuals including travellers, and they should also be considered as important etiologic factors of travellers' diarrhoea.

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