Green synthesized AgNPs of the aqueous extract resulting in impressive protein kinase, antioxidant, antibacterial, and antifungal activities.

This study focused on analyzing the pharmacological activities of AgNPs synthesized from an aqueous plant extract of . The effectiveness of AgNPs was evaluated for protein kinase inhibition, antioxidant, antibacterial, and antifungal activities. The AgNPs and plant were used to regulate the protein kinase activity using the liquid TSB and ISP4 medium protein kinase inhibition study demonstrated that nanoparticles exhibited a larger zone of inhibition (9.

Antimicrobial Resistance and Associated Risk Factors for in Patients Attending Tertiary Care Settings.

To determine the incidence of antimicrobial-resistant emerging pathogens, , and its associated risk factors in tertiary care setups of Pakistan. This cross-sectional prospective study was conducted from January 2019 to December 2020, to determine the prevalence and antimicrobial resistance patterns of strains isolated from 450 stool specimens of patients suffering from diarrhea hospitalized in tertiary care hospitals in Peshawar, Pakistan. The stool samples of the patients were processed for culture and detection of toxin A and toxin B by enzyme-linked immunosorbent assay (ELISA) and PCR.

Incidence of Helminthic and Viral Coinfections in Malaria Patients in the Tertiary Care Hospital Setup.

Introduction: This study determines the incidence of common viral and helminth coinfections with malaria in the tertiary care hospital set up in southern Khyber Pakhtunkhwa, Pakistan.

Materials And Methods: The multidimensional research included malaria patients admitted to different hospitals of district Kohat during January and December 2021. Stool samples and blood were assembled from the patients.

Multiple-Drug Resistant Shiga Toxin-Producing in Raw Milk of Dairy Bovine.

Introduction: Raw milk may contain pathogenic microorganisms harmful to humans, e.g., multidrug-resistant non-O157:H7, which can cause severe colitis, hemolytic uremia, and meningitis in children.

Lipid-based Nutritional Supplement Impact on Energy Intake, Appetite, Glucose and Insulin Levels in Under-Weight Pregnant and Lactating Women with Preeclampsia.

Objective: To investigate the response of nutritional supplement (LNS-PLW) on appetite score, energy intake, insulin and glucose levels in preeclamptic women.

Design & Participants: Sixty under-weight preeclamptic primigravida were divided into two groups randomly and provided LNS-PLW/Placebo in the fasted state. Blood samples were collected at fasting state, after 30mins of supplementation, "ad libitum buffet" breakfast and lunch for glucose and insulin levels.

Biological Control of Hyalomma Ticks in Cattle by Fungal Isolates.

Ticks pose a major threat to cattle health and production in Pakistan because they transmit pathogens of diseases like Babesiosis and Theileriosis. spp., found across Africa, Asia, and Europe, are especially problematic.

Phytosynthesized Iron Oxide Nanoparticles Using Aqueous Extract of (Hardy Sugar Cane), Their Characterizations, Antiglycation, and Cytotoxic Activities.

The goal of the current study is to achieve plant-mediated synthesis of iron oxide nanoparticles (FeO NPs). The plant extract of was used as a reducing and stabilizing agent for the synthesis of FeO NPs. Different techniques such as energy-dispersive X-ray analysis (EDX), X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and UV-visible spectroscopy (UV-vis) were used to characterize the synthesis of FeO NPs.

Revolutionizing Nanotechnology with Extracts: Biogenic Synthesis of Silver Nanoparticles Exhibiting Potent Antioxidant and Antibacterial Activities.

In this study, we described the environmentally friendly biosynthesis of silver nanoparticles (AgNPs) utilizing ethanolic extract of () as a capping and reducing agent. We also looked at the antioxidant and antibacterial capacities of AgNPs. In order to determine the size, shape, and crystallinity of the created AgNPs, the current project was designed to produce AgNPs utilizing the crude extract of the .

6-Aminoflavone Activates Nrf2 to Inhibit the Phospho-JNK/TNF-α Signaling Pathway to Reduce Amyloid Burden in an Aging Mouse Model.

In the current study, we examined the antioxidant activity and anti-amyloidogenic potential of 6-aminoflavone in an adult mice model of d-galactose-induced aging. Male albino eight-week-old mice were assigned into four groups: 1. the control group (saline-treated), 2.

Biofabrication of Titanium Dioxide Nanoparticles Catalyzed by : Characterization and Evaluation of their Antiepileptic and Cytotoxic Activities.

The green synthesis of nanoparticles using plant extract is a new method that can be used in various biomedical applications. Therefore, the green approach was an aspect of ongoing research for the synthesis titanium dioxide nanoparticles (TiO NP) using the aqueous plant extract, which acts as a stabilizing and reducing agent. The synthesis of TiO NPs was confirmed by energy dispersive X-ray (EDX), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and UV-visible spectroscopy (UV-vis) analyses.

Antiplatelet, cytotoxic activities and characterization of green-synthesized zinc oxide nanoparticles using aqueous extract of Nephrolepis exaltata.

The goal of the current study was to synthesize zinc oxide nanoparticles (ZnO-NPs) using ZnCl.2HO salt precursor and an aqueous extract of Nephrolepis exaltata (N. exaltata), which act as a capping and reducing agent.

Whole Exome Sequence Analysis for Inborn Errors of IL-12/IFN- Axis in Patient with Recurrent Typhoid Fever.

Background: The IL-12/IFN- axis pathways play a vital role in the control of intracellular pathogens such as .

Objective: The study is aimed at using whole exome sequencing (WES) to screen out genetic defects in IL-12/IFN- axis in patients with recurrent typhoid fever.

Methods: WES using next-generation sequencing was performed on a single patient diagnosed with recurrent typhoid fever.

Biallelic mutations in FLG, TGM1, and STS genes segregated with different types of ichthyoses in eight families of Pakistani origin.

Background: Congenital ichthyosis is a diverse group of keratinization disorders associated with generalized scaling of skin of varying severity. The non-syndromic forms of congenital ichthyosis are further grouped into common ichthyosis (ichthyosis vulgaris and X-linked ichthyosis), autosomal recessive congenital ichthyosis, and keratopathic ichthyosis.

Objective: To identify sequence variants involved in different forms of hereditary ichthyoses.

Effect of Lipid-Based Multiple Micronutrients Supplementation in Underweight Primigravida Pre-Eclamptic Women on Maternal and Pregnancy Outcomes: Randomized Clinical Trial.

Background and Objectives: In pre-eclampsia, restricted blood supply due to the lack of trophoblastic cell invasion and spiral artery remodeling is responsible for adverse pregnancies and maternal outcomes, which is added to by maternal undernutrition. This study was designed to observe the effect of multiple nutritional micronutrient supplements on the pregnancy outcomes of underweight pre-eclamptic women. To investigate the effects of lipid-based multiple micr supplementations (LNS-PLW) on pregnancy and maternal outcomes in underweight primigravida pre-eclamptic women.

Comparative Study of Antimicrobial Activity of Silver, Gold, and Silver/Gold Bimetallic Nanoparticles Synthesized by Green Approach.

Nanotechnology is one of the most recent technologies. It is uncertain whether the production of small-size nanoparticles (NPs) can be achieved through a simple, straightforward, and medicinally active phytochemical route. The present study aimed to develop an easy and justifiable method for the synthesis of Ag, Au, and their Ag/Au bimetallic NPs (BNPs) by using (HH) extract, and then to investigate the effects of Ag, Au, and their Ag/Au BNPs as antimicrobial and phytotoxic agents.

Characterization, Antiplasmodial and Cytotoxic Activities of Green Synthesized Iron Oxide Nanoparticles Using Aqueous Extract.

The use of non-toxic synthesis of iron oxide nanoparticles (FeO NPs) by an aqueous plant extract has proven to be a viable and environmentally friendly method. Therefore, the present investigation is based on the FeO NPs synthesis by means of FeCl·6HO as a precursor, and the plant extract of () serves as a capping and reducing agent. Various techniques were used to examine the synthesized FeO NPs, such as UV-Visible Spectroscopy (UV-Vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Energy Dispersive X-ray (EDX).

Characterization and Evaluation of Antimicrobial Potential of (Linn) Mediated Biosynthesized Silver Nanoparticles.

The goal of the research was to explore a new green method used to synthesize silver nanoparticles (Ag NPs) from an aqueous extract of , which serves as a reducing and stabilizing agent. The obtained results showed an 85% yield of nanoparticles by using 2:5 (/) of 5% plant extract with a 0.5 M solution of AgNO.

Detection of Novel Biallelic Causative Variants in Gene by Whole-Exome Sequencing, Resulting in Congenital Recessive Dystrophic Epidermolysis Bullosa in Three Unrelated Families.

Background: Dystrophic Epidermolysis bullosa (DEB) is a rare, severe subtype of epidermolysis bullosa (EB), characterized by blisters and miliary rashes of the skin. Dystrophic EB (DEB) includes variants inherited both in an autosomal-dominant or autosomal-recessive manner. Recessive dystrophic EB (RDEB) is divided into many subtypes and prevails as a result of biallelic genetic mutations in COL7A1 gene encoding type VII collagen, a major stabilizing molecule of the dermo-epidermal junction.

Urease and Chymotrypsin Inhibitory Activities and Molecular Docking Studies of Alkaloids Isolated from Medicinal Plant Bunge.

Phytochemical studies on the alkaloids fraction of the entire plant of Bunge resulted in the alkaloids isolation, which were first time isolated from this species. The 1D and 2D NMR spectroscopic data were used to identify their structures, and there was satisfactory compatibility of the data compared to those which were previously published. In the examined compounds , Isaindigotidione () and Isaindigotone () were shown as an effective urease inhibitor in such a concentration-dependent way against Jack bean and urease, with IC values 29.

Profile of Medicinal Plants Traditionally Used for the Treatment of Skin Burns.

Moroccan folk healers use medicinal plants to treat several diseases including skin burns. The traditional knowledge of wound healing is not common among the general population. Only one ethnobotanical survey was carried out in Rabat, Morocco, to track the traditional use of medicinal plants in wound healing.

Chemical Composition, Antioxidant, Insecticidal Activity, and Comparative Analysis of Essential Oils of Leaves and Fruits of and .

Raddi. and L. are perennial woody plants belonging to the Anacardiaceae family, widely distributed in the United States, Europe, Asia, and Africa, and they are broadly used for many applications such as in traditional medicine as an antipyretic, analgesic, depurative, and in the treatment of diseases of the urogenital system as well as culinary and ornamental species.

Novel splicing-site mutation in DCAF17 gene causing Woodhouse-Sakati syndrome in a large consanguineous family.

Background: Woodhouse-Sakati syndrome is a rare autosomal recessive disease with endocrine and neuroectodermal aberrations with heterogeneous phenotypes and disease course. The most common phenotypes of the disease are progressive sensorineural hearing loss and alopecia, mild-to-moderate mental retardation and hypogonadism. The disease results from mutations in the DCAF17 gene.