Assessing the distribution of cancer stem cells in tumorspheres.

Cancer Stem Cells presumably drive tumor growth and resistance to conventional cancer treatments. From a previous computational model, we inferred that these cells are not uniformly distributed in the bulk of a tumorsphere. To confirm this result, we cultivated tumorspheres enriched in stem cells, and performed immunofluorescent detection of the stemness marker SOX2 using confocal microscopy.

Effects of a differentiating therapy on cancer-stem-cell-driven tumors.

The growth of many solid tumors has been found to be driven by chemo- and radiotherapy-resistant cancer stem cells (CSCs). A suitable therapeutic avenue in these cases may involve the use of a differentiating agent (DA) to force the differentiation of the CSCs and of conventional therapies to eliminate the remaining differentiated cancer cells (DCCs). To describe the effects of a DA that reprograms CSCs into DCCs, we adapt a differential equation model developed to investigate tumorspheres, which are assumed to consist of jointly evolving CSC and DCC populations.

[Answers and latencies dichotic digit test normoacoustic majoring in hearing].

Neurocognitive assessment by dichotic digit test provides selective stimulation of auditory pathway with contralateral suppression of the ipsilateral showing interhemispheric differences in concurrent tasks. In order to recognize the pattern of responses, recovery order of digits and latencies heard the original test was modified with the addition of a record of an audio track of the responses. The sample includes subjects with a history in hearing specialization linked to the music and listen to comprehensive second language, normoacoustic without otologic diseases or neurological.