Myxoid adrenal cortical carcinoma. A case report with differential diagnostic considerations.

The presentation of an adrenal cortical carcinoma with myxoid ground substance is an unusual and apparently rare event. To my knowledge, only one such case has been previously reported in the literature. A second case of a metastasizing adrenal cortical carcinoma with abundant acidic mucosubstances and a myxomalike appearance is reported herein.

Optimization of DNA extraction from formalin-fixed tissue and its clinical application in Duchenne muscular dystrophy.

The authors report a case of Duchenne muscular dystrophy in which a deletion was determined by multiplex polymerase chain reaction using postmortem tissue from a proband who died in 1985, 2 years before the cloning of the dystrophin gene. Several extraction methods were analyzed to determine optimal conditions for recovery of DNA from fixed tissue. Variables examined were tissue lysis times and temperatures, a simple salting-out procedure for purification of DNA, polymerase chain reaction amplification of crude lysate versus purified DNA, and lysis of different tissues and tissue quantities.