Publications by authors named "Fong L Pan"

Article Synopsis
  • CAR-T therapy is a new way to treat blood cancers and is being looked at for other diseases and issues like solid tumors and aging.
  • Traditional methods to create these CAR-T cells can hurt the cells or cause problems, making them less safe and effective.
  • A new method called hydroporation helps make more healthy CAR-T cells quickly and efficiently, which could lead to better treatments for patients.
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Article Synopsis
  • CAR-T therapy is revolutionary for treating blood cancers and is being explored for other conditions, but faces manufacturing and safety issues.
  • Traditional methods for cell engineering can lead to random gene placement or cellular damage, which can affect therapy effectiveness.
  • Hydroporation is introduced as a more effective method, yielding higher quality CAR-T cells with better growth and performance, and can be scaled up quickly for large production needs.
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Microfluidic vortex shedding (µVS) can rapidly deliver mRNA to T cells with high yield and minimal perturbation of the cell state. The mechanistic underpinning of µVS intracellular delivery remains undefined and µVS-Cas9 genome editing requires further studies. Herein, we evaluated a series of µVS devices containing splitter plates to attenuate vortex shedding and understand the contribution of computed force and frequency on efficiency and viability.

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