Surface modification of SOI-FET sensors for label-free and specific detection of short RNA analyte.

Aim: A new type of surface modification of SOI-FET sensors with ultrathin sensor-probe transition layer and uncharged probes for highly specific detection of short RNA (saRNA) was suggested.

Materials & Methods: Carbonyldiimidazole (CDI) or glycidoxypropyltrimethoxysilane were used as precursors of sensor-probe interface layers, together with peptide nucleic acids and new NA analogues - phosphoryl guanidine oligo(2'-OMe)ribonucleotides (PGO) as probes for RNA hybridization. RNA sequences corresponding to mRNA NELFA (NM_005663) and microRNA-29a (cancer markers) were used as saRNA targets.

[Registration of the protein in the serum with a field-effect nanotransistor biosensor].

A method for detection of cancer-associated protein D-NFATc1 in serum using nanowire (NW) biosensor based on field-effect nanotransistor is developed. Field-effect nanotransistor was fabricated on the basis of «silicon-on-insulator» structures. For the biospecific detection of target protein, the NW surface was modified with aptamers against the target protein.

[SOI-nanowire biosensor for the detection of D-NFAT 1 protein].

The nanowire (NW) detection is one of fast-acting and high-sensitive methods allowing to reveal potentially relevant protein molecules. A NW biosensor based on the silicon-on-insulator (SOI)-structures was used for biospecific label-free detection of NFAT 1 (D-NFAT 1) oncomarker in real time. For this purpose, SOI-nanowires (NWs) were modified with aptamers against NFAT 1 used as molecular probes.

SOI nanowire for the high-sensitive detection of HBsAg and α-fetoprotein.

Silicon-on-isolator-nanowires (SOI-NWs) were used for the label-free, real-time biospecific detection of the hepatitis B marker HBsAg and cancer marker α-fetoprotein (AFP). Specific protein-protein recognition was carried out using individual NWs that were functionalized with antibodies. To solve the problem of non-specific binding of target protein molecules to the sensor element the use of a reference NW with immobilized antibodies against non-target proteins was proposed.

[Classification of the avian summer population of the Western Siberian plain].

The proposed classification system reflects the difference between the three population systems: unbuilt land, built-up land, and aquatic-semiaquatic communities. Two superorder groups--north and median--further divided into types were recognized in each of the systems. Most types are divided into subtypes, classes, and subclasses (and sometimes genera of the population).

[The use of inflow-outflow drainage after cerebral operations].

The paper provides strong evidence for the use of inflow-outflow drainage after brain operations and presents an analysis of 255 cerebral operations using various draining modes. The inflow-outflow drainage after brain operations is found to be promising in terms of clinical manifestations. The authors propose their own draining tube for inflow-outflow drainage.

[Development of a method for identifying bovine and human types of mycobacteria using the polymerase chain reaction].

An express method has been elaborated for identification of human and bovine species mycobacteria in the polymerase chain reaction. The high specificity of the technique and simplicity of material preparation for research make the experimental procedure simple and quick, permitting one to identify Mycobacterium tuberculosis and Mycobacterium bovis directly in pathogenic material.

[Legal regulations of medical activities].

The article contains an analysis of some provisions of the existing health legislation. At the same time a short statement is given of civil and criminal-judicial procedures which make provisions for some professional duties of physicians. It is supposed that the performance of these duties would require additional legal knowledge for example, in the field of hereditary law.

[Description of conditioned reflex elaboration in cats in response to electric stimulation of the hippocampal formation].

In six cats with chronically implanted brain electrodes conditioned running to the feeding trough was elaborated in response to electrical stimulation of the ventral hippocampal formation (VHF), which at first produced inhibition of running. The stages of conditioning were as follows: 1) inhibition of conditioned activity; 2) replacement of inhibition by more frequent runnings--generalization of the conditioned reflex; 3) enhancement of signal significance of VHF electrical stimulation and subsequent decrease of intersignal reactions. Conditioned reflex to electrical stimulation of CA1 field was elaborated slower than that to electrical stimulation of other VHF points.

[Frequency of self stimulation in cats when two parameters are changed simultaneously].

In cats, with electrodes implanted in the lateral hypothalamus a dependence was obtained of self-stimulation frequency (SSF) on paired changes of current strength and each of other stimulation parameters-duration, pulse duration and frequency. In some cases these parameters may independently affect SSF but generally they are in interaction. For stimulation with electrodes located at the level of the hypothalamic ventromedial nucleus, two-peak dependences of SSF on the current strength and the duration of stimulation pulses were obtained.

Investigation of phase-growth kinetics during interaction of silicon single crystals and molybdenum thin films.

Investigation of the phase-growth kinetics and identification of the phases formed as a result of reactive diffusion in the system of Si single crystals and Mo thin films, has been carried out by electrochemical anodic etching.