Development and Synthesis of Bombesin-Based Radiopharmaceutical Precursors Modified with Knottin.

Unlabelled: Bombesin receptors on the cell surface are of great interest as a target for targeted cancer therapy. One of the strategies of targeting bombesin receptors involves the use of tropic short peptides. However, the main limitation for the wide application of peptides as drugs is their low stability due to their sensitivity to extreme conditions of the internal body environment such as temperature and action of enzymes.

Relationships of Thickness of Perirenal Fat with Urinary Levels of MCP-1 and NGAL in Patients with Hypertension.

Background: We conducted a study to determine the relationships between perirenal fat (PRF) thickness and urinary levels of monocyte chemoattractant protein-1 (MCP-1) and neutrophil gelatinase-associated lipocalin (NGAL) in patients with hypertension (HTN).

Methods: In 338 HTN patients (aged 63.5±12.

Meso-Macroporous Hydroxyapatite Powders Synthesized in Polyvinyl Alcohol or Polyvinylpyrrolidone Media.

Mesoporous hydroxyapatite (HA) is widely used in various applications, such as the biomedical field, as a catalytic, as a sensor, and many others. The aim of this work was to obtain HA powders by means of chemical precipitation in a medium containing a polymer-polyvinyl alcohol or polyvinylpyrrolidone (PVP)-with concentrations ranging from 0 to 10%. The HA powders were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, atomic emission spectroscopy with inductively coupled plasma, electron paramagnetic resonance, scanning electron microscopy (SEM), and transmission electron microscopy (TEM).

Peculiarities of charge compensation in lithium-doped hydroxyapatite.

Hydroxyapatite (HA) remains one of the most popular materials for various biomedical applications and its fields of application have been expanding. Lithium (Li) is a promising candidate for modifying the biological behavior of HA. Li is present in trace amounts in the human body as an alkaline and bioelectric material.

Stabilizing Scaffold for Short Peptides Based on Knottins.

Background: Bombesin (BBN) is a short peptide with a high affinity for receptors that are expressed on the surface of various types of cancer cells. However, a full length BBN molecule has low stability.

Objective: In our study, we propose the use of peptide toxins, derived from animal and plant toxins, as scaffold molecules to enhance the bioavailability and stability of bombesin.

Protective Effect of Peptide Calcium Channel Blocker Omega-Hexatoxin-Hv1a on Epithelial Cell during Ischemia-Reperfusion Injury.

Ischemia-reperfusion injury (IRI) is a common phenomenon that develops both from natural causes and during major operations. Many intracellular processes mediated by calcium ions are involved in the development of IRI. Currently, chemical calcium channel blockers are used but they have a number of limitations.

[Subthreshold micropulse laser treatment of the foveal region in chronic central serous chorioretinopathy (case study)].

The article presents a clinical case of subthreshold micropulse laser (SML) treatment of chronic central serous chorioretinopathy (CSC) in the left eye of a 44-year-old female patient . with dye allergy. The patient first noticed a gray spot, distortion of objects in February, 2018.

Zn-Doped Calcium Magnesium Phosphate Bone Cement Based on Struvite and Its Antibacterial Properties.

The development of magnesium calcium phosphate bone cements (MCPCs) has garnered substantial attention. MCPCs are bioactive and biodegradable and have appropriate mechanical and antimicrobial properties for use in reconstructive surgery. In this study, the cement powders based on a (Ca + Mg)/P = 2 system doped with Zn at 0.

Effect of the Peptide Calcium Channel Blocker ω-hexatoxin-Hv1a on Cell Death during Ischemia/Reperfusion .

Unlabelled: Apoptosis and necrosis during reperfusion after ischemia are key mechanisms at the cellular level leading to damage. The development of pathological conditions is preceded by intracellular calcium ion overload both at the stage of ischemia and at the stage of reperfusion. In this regard, one of the strategies aimed at reducing damage during ischemia/reperfusion is associated with the use of calcium channel blockers.

Optically stimulated electron paramagnetic resonance: Simplicity, versatility, information content.

A simple technique for observing optically stimulated electron paramagnetic resonance (OSEPR) is proposed and investigated. The versatility and information content of the described technique is demonstrated by the example of the OSEPR spectra of systems that are unpopular for this type of spectroscopy: a crystal with rare-earth ions Nd and a doped semiconductor GaAs. In addition, the OSEPR spectrum of atomic cesium is presented, in which an optical nonlinearity is observed that makes it possible to estimate the Rabi frequency for the relevant optical transition.

Peptide Sodium Channels Modulator Mu-Agatoxin-Aa1a Prevents Ischemia-Reperfusion Injury of Cells.

Ischemia-reperfusion injury (IRI) is an irreversible functional and structural injury. Restoration of normal oxygen concentration exacerbates the emergence and development of deadly cells. One of the possible moments of reperfusion damage to cells is an increase in the intracellular concentration of sodium ions.

[Widefield optical coherence tomography as an effective method for detecting imperceptible flap retinal tears (clinical observation)].

Certain late peripheral ruptures after acute posterior vitreous detachment (PVD) may remain undetected during the initial examination of the fundus due to the presence of vitreous hemorrhages and other factors, but they can lead to rhegmatogenic retinal detachment in the future. Hence, it is necessary to conduct an additional thorough examination using optical coherence tomography (OCT) of the vitreoretinal interface periphery in the presence of tear predictors: retinal and vitreal hemorrhages, vitreous pigment cells ("tobacco dust") in the anterior segment of the vitreous body, as well as hyper-reflective dots ("starry sky") in the vitreous body according to OCT scans of patients with acute symptomatic detachment of the hyaloid membrane. The article presents the case of a patient with a clinically imperceptible flap tear detected during acute posterior vitreous detachment.

Effects of Various Ripening Media on the Mesoporous Structure and Morphology of Hydroxyapatite Powders.

Mesoporous hydroxyapatite (HA) materials demonstrate advantages as catalysts and as support systems for catalysis, as adsorbent materials for removing contamination from soil and water, and as nanocarriers of functional agents for bone-related therapies. The present research demonstrates the possibility of the enlargement of the Brunauer-Emmett-Teller specific surface area (SSA), pore volume, and average pore diameter via changing the synthesis medium and ripening the material in the mother solution after the precipitation processes have been completed. HA powders were investigated via chemical analysis, X-ray diffraction analysis, Fourier-transform IR spectroscopy, transmission electron microscopy (TEM), and scanning (SEM) electron microscopy.

Biological activity of silver nanoparticles synthesized with Paenibacillus polymyxa exopolysaccharides.

Recently, there has been increased interest in the synthesis of nanoparticles by using natural polysaccharides. These polysaccharides are eco-friendly, nontoxic, and cheap to prepare. On the other hand, the attention in hydrocolloids and films has significantly enhanced, and their application is very promising in the food, pharmaceutical, perfumery and cosmetics, oil, paper, and textile industries.

Photoplethysmographic Imaging of Hemodynamics and Two-Dimensional Oximetry.

The review of recent papers devoted to actively developing methods of photoplethysmographic imaging (the PPGI) of blood volume pulsations in vessels and non-contact two-dimensional oximetry on the surface of a human body has been carried out. The physical fundamentals and technical aspects of the PPGI and oximetry have been considered. The manifold of the physiological parameters available for the analysis by the PPGI method has been shown.

Separate extraction of human eccrine sweat gland activity and peripheral hemodynamics from high- and low-quality thermal imaging data.

Unlabelled: Sweat gland activity and peripheral hemodynamics, which characterize the function of sympathetic cholinergic nerve fibers and the manifestation mechanisms of vascular tone regulation, respectively, can be detected via dynamic thermography of the skin. Thus, they are useful parameters for diagnosing various forms of neuropathy and functional circulatory disorders. Both parameters affect the dynamics of the skin temperature; therefore, for an adequate description of thermographic data, it is necessary to build models that consider both these coexisting components simultaneously.

Immunization of Mice with Gold Nanoparticles Conjugated to Thermostable Cancer Antigens Prevents the Development of Xenografted Tumors.

Gold nanoparticles as part of vaccines greatly increase antigen stability, antigen accumulation in the lymph nodes, and antigen uptake by antigen-presenting cells. The use of such particles as part of anticancer vaccines based on heat shock proteins to increase vaccine effectiveness is timely. We prepared and characterized nanoconjugates based on 15-nm gold nanoparticles and thermostable tumor antigens isolated from MH22a murine hepatoma cells.

[The research activity of students in "The public health and health care, health care economics" discipline].

The article considers research work of students in "The Public health and health care, health economics" discipline in 2001-2020, organized in course of public health and health care of the Medical Institute of Petrozavodsk State University. The article reveals such concepts as student scientific activity, knowledge requirements for discipline to prepare student research work, classification of student research works by degree of collection and analysis of material, levels of student work performance, stages and classes of social hygienic research, requirements for content of presentation, criteria of evaluating report and presentation. During 2001-2021, 250 students of medical institute in the section "Public health and health care" made more than 200 reports, published more than 20 thesis and articles.

Synthesis of silymarin-selenium nanoparticle conjugate and examination of its biological activity in vitro.

Silymarin (Sil) was conjugated to selenium nanoparticles (SeNPs) to increase Sil bioavailability. The conjugates were monodisperse; the average diameter of the native SeNPs was ~ 20-50 ± 1.5 nm, whereas that of the conjugates was 30-50 ± 0.

Influence of Synthesis Conditions on Gadolinium-Substituted Tricalcium Phosphate Ceramics and Its Physicochemical, Biological, and Antibacterial Properties.

Gadolinium-containing calcium phosphates are promising contrast agents for various bioimaging modalities. Gadolinium-substituted tricalcium phosphate (TCP) powders with 0.51 wt% of gadolinium (0.

Sensor System Based on a Piezoelectric Resonator with a Lateral Electric Field for Virus Diagnostics.

A sensor system based on a piezoelectric resonator with a lateral electric field in the frequency range 6-7 MHz of the electric field for virus detection is described. Through use of the transmissible virus causing gastroenteritis in pigs and specific antibodies, the possibility of detecting the virus in suspension in real time was determined. It was found that the frequency dependence of the real and imaginary parts of the electrical impedance of such a resonator loaded with a virus suspension changes significantly after the addition of specific antibodies to the suspension.

OCT visualization of hyporeflective lacuna in the retrolental space.

Purpose: To investigate the vitreo-lenticular interface and anterior vitreous using high-resolution optical coherence tomography (OCT).

Material And Methods: This prospective single-center pilot study included 34 patients (68 eyes) - 6 males and 28 females with a mean age of 58.4±12.

Proteins and polysaccharides from vegetative mycelium of medicinal basidiomycete Lentinus edodes display cytotoxicity towards human and animal cancer cell lines.

Detection and study of biologically active compounds seems a promising area of research in cancer diagnostics and therapies. The glycoprotein and polysaccharide fractions showing high cytotoxicity towards several human and animal cancer cell lines: A549, Hep-2, HeLa, С6 and SPEV-2 were isolated from basidiomycete Lentinus edodes vegetative mycelium and fruiting body and further characterized. It was found that water-soluble glycoprotein fractions caused the most significant, 70-100% inhibition of metabolic activity of SPЕV-2, А549 and С6 cell lines.

Federal medical biological agency of Russia's efforts to support Russian athletes during COVID-19 outbreak.

This paper dwells upon COVID-19-related efforts of the Center for Sports Medicine, Federal Medical and Biological Agency of Russia. The Agency has the following precautions in place: regular polymerase chain reaction (PCR) testing of athletes and staff; double PCR testing before going to training camps or medical examinations; isolating athletes and their traceable contacts when COVID is suspected; observation and isolation wards set up at training camp venues. Athlete vaccination has begun.