Use of CRISPR interference for efficient and rapid gene inactivation in .

Gene inactivation by creating in-frame deletion mutations in is time consuming, and most fusobacterial strains are genetically intractable. Addressing these problems, we introduced a riboswitch-based inducible CRISPR interference (CRISPRi) system. This system employs the nuclease-inactive Cas9 protein (dCas9), specifically guided to the gene of interest by a constantly expressed single-guide RNA (sgRNA).

Use of CRISPR interference for efficient and rapid gene inactivation in .

Unlabelled: Gene inactivation via creating in-frame deletion mutations in is time-consuming, and most fusobacterial strains are genetically intractable. Addressing these problems, we introduced a riboswitch-based inducible CRISPRi system. This system employs the nuclease-inactive Cas9 protein (dCas9), specifically guided to the gene of interest by a constantly expressed single guide RNA (sgRNA).