Surfactants' Interplay with Biofilm Development in and .

The capacity of micro-organisms to form biofilms is a pervasive trait in the microbial realm. For pathogens, biofilm formation serves as a virulence factor facilitating successful host colonization. Simultaneously, infections stemming from biofilm-forming micro-organisms pose significant treatment challenges due to their heightened resistance to antimicrobial agents.

Increased Absorption and Inhibitory Activity against spp. of Imidazole Derivatives in Synergistic Association with a Surface Active Agent.

This paper's purpose was to evaluate the interaction between three imidazole derivatives, (2-methyl-1-imidazol-1-yl)methanol (SAM3), 1,1'-methanediylbis(1-benzimidazole (AM5) and (1-benzo[d]imidazol-1-yl)methanol 1-hydroxymethylbenzimidazole (SAM5) on the one hand, and sodium dodecyl sulphate (SDS) on the other, as antifungal combinations against spp. Inhibitory activity was assessed using the agar diffusion method and Minimal Inhibitory Concentration (MIC) and showed moderate inhibitory activity of single imidazole derivatives against spp. The mean value of MIC ranged from 200 µg/mL (SAM3) to 312.

Multiple Assays on Non-Target Organisms to Determine the Risk of Acute Environmental Toxicity in Tebuconazole-Based Fungicides Widely Used in the Black Sea Coastal Area.

The widespread use of Tebuconazole-based fungicides in phytosanitary treatments on a wide range of crops, on the one hand, and the lack of official reports on the amount of fungicide residues in nearby water basins, on the other hand, may lead to uncontrolled and hazardous contamination of water sources used by the resident population, and to serious effects on the environment and public health. Our study explores the acute toxicological risk of this fungicide on various organisms, from bacteria and yeast to fish, using a battery of tests (standardized Toxkit microbiotests and acute semi-static tests). By investigating the interaction between Tebuconazole and bacteria and yeast organisms, we observed that Gram-negative bacteria displayed a strong tolerance for Tebuconazole, while Gram-positive bacteria and yeasts proved to be very sensitive.

Chemical Composition, Antioxidant Activity, and Antibacterial Activity of Black Poplar Buds' Hydroalcoholic Macerates from Dobrogea Area.

Black poplar buds have high contents of many compounds with therapeutic potential, which are useful in cosmetics and the treatment of various dermatitis, respiratory diseases, etc. The aim of this study was to identify and exploit the local plant resources with biologically active properties from the Dobrogea area, Romania. For this purpose, materials were collected from the mentioned area, and macerates of black poplar were prepared in order to evaluate their qualities as antioxidant and antimicrobial agents.

Polysorbate 21 Can Modulate the Antibacterial Potential of Two Pyrazol Derivatives.

The combination of two compounds with known antimicrobial activity may, in some cases, be an effective way to limit the resistance to antibiotics of specific pathogens. Molecules carrying pyrazole moiety are well known for their bioactive properties and have wide applicability in the medical and pharmaceutical field. Surfactants have, among other useful properties, the ability to affect the growth of microorganisms.

Effect of methylpyrazoles and coumarin association on the growth of Gram-negative bacteria.

One approach to overcome the antimicrobial resistance of many pathogens is to associate compounds with antimicrobial properties and obtain combinations superior compared to the effect of each compound. To identify a possible potentiating effect, we tested and analyzed the inhibitory effect of the combination of coumarin with two pyrazole derivatives, 1,1'-methandiylbis (3,5-dimethyl-1H-pyrazole (AM4) and 3,5-dimethyl-1H-pyrazol-1-yl) methanol 3,5-dimethyl-1-hydroxymethylpyrazol (SAM4). A clear synergistic effect was recorded when coumarin was associated with SAM4, in which case the Fractional Inhibitory Concentration Index (FICI) had a value equal to 0.

Inhibitory potential of a novel imidazole derivative as evaluated by time-kill and dehydrogenase activity.

Antibacterial activity of 1,1'-methandiylbis(2-methyl-1H-imidazole) (AIM) has been estimated both qualitatively and quantitatively against reference and clinical strains of Gram-positive and Gram-negative bacteria. MICs showed little variability among strains tested, ranging from 360 to 450 μg/ml and indicating rather a moderate antibacterial activity. Inhibition of dehydrogenase activity was significant in Escherichia coli ATCC 25922 and followed closely time-kill dynamics.