Development of a standardised human in vitro digestion protocol based on macronutrient digestion using response surface methodology.

Bioaccessibility studies should be taken into account when evaluating the physiological effects of ingested compounds at the intestine level. Several in vitro digestion protocols have been described, with a wide range of experimental conditions but no optimised protocol exists. In order to fill in this gap, we evaluated the influence of three continuous factors (pH, incubation time, and enzyme concentrations), in the range of values found in literature, on the digestion of standard macronutrients (starch, albumin, triolein) alone or in mixture.