Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens.

Diffraction-limited deep focusing into biological tissue is challenging due to aberrations that lead to a broadening of the focal spot. The diffraction limit can be restored by employing aberration correction for example with a deformable mirror. However, this results in a bulky setup due to the required beam folding.

Aspherical high-speed varifocal mirror for miniature catadioptric objectives.

We present a varifocal mirror based on a piezo-actuated glass membrane that can be used as a secondary mirror in miniature Cassegrain-type mirror- or catadioptric objectives. The mirror section has a diameter of 10 mm on a clear membrane diameter of 23 mm, with a focal range of ±8 m and a response time on the millisecond-scale. The two piezo layers enable an aspherical tuning range that covers the elliptic, parabolic and hyperbolic regime over most of the focal range.