Hepatitis B virus (HBV) genotype determination by the COBAS AmpliPrep/COBAS TaqMan HBV Test, v2.0 in serum and plasma matrices.

Background: Viral load quantification is established in the clinical management of chronic Hepatitis B virus (HBV) infection for assessing efficacies and resistance developments in anti-viral drug treatment.

Objectives: The fully automated COBAS AmpliPrep/COBAS TaqMan HBV Test, v2.0 was evaluated for the linear measuring range and the inclusivity of HBV genotype determination in EDTA plasma and serum samples.

Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor.

Cytoplasmic sorting of mRNAs by microtubule-based transport is widespread, yet very little is known at the molecular level about how specific transcripts are linked to motor complexes. In Drosophila, minus-end-directed transport of developmentally important transcripts by the dynein motor is mediated by seemingly divergent mRNA elements. Here we provide evidence that direct recognition of these mRNA localization signals is mediated by the Egalitarian (Egl) protein.