Conformational regulation and target-myristoyl switch of calcineurin B homologous protein 3.

Calcineurin B homologous protein 3 (CHP3) is an EF-hand Ca-binding protein involved in regulation of cancerogenesis, cardiac hypertrophy, and neuronal development through interactions with sodium/proton exchangers (NHEs) and signalling proteins. While the importance of Ca binding and myristoylation for CHP3 function has been recognized, the underlying molecular mechanism remained elusive. In this study, we demonstrate that Ca binding and myristoylation independently affect the conformation and functions of human CHP3.

The reliance of glycerol utilization by Cupriavidus necator on CO fixation and improved glycerol catabolism.

While crude glycerol is a cheap carbon source for industrial-scale cultivation of microorganisms, its application relies on fast growth and conversion. The biopolymer producing Cupriavidus necator H16 (synonym: Ralstonia eutropha H16) grows poorly on glycerol. The heterologous expression of glycerol facilitator glpF, glycerol kinase glpK, and glycerol dehydrogenase glpD from E.

Handheld Spectral Sensing Devices Should Not Mislead Consumers as Far as Non-Authentic Food Is Concerned: A Case Study with Adulteration of Milk Powder.

With the rising trend of consumers being offered by start-up companies portable devices and applications for checking quality of purchased products, it appears of paramount importance to assess the reliability of miniaturized sensors embedded in such devices. Here, eight sensors were assessed for food fraud applications in skimmed milk powder. The performance was evaluated with dry- and wet-blended powders mimicking adulterated materials by addition of either ammonium sulfate, semicarbazide, or cornstarch in the range 0.

Variational adaptive correlation method for flow estimation.

A variational approach is presented to the estimation of turbulent fluid flow from particle image sequences in experimental fluid mechanics. The approach comprises two coupled optimizations for adapting size and shape of a Gaussian correlation window at each location and for estimating the flow, respectively. The method copes with a wide range of particle densities and image noise levels without any data-specific parameter tuning.