Multiparameter analysis of human oocytes at metaphase II stage after IVF failure in non-male infertility.

Background: Using fluorescence imaging, an a posteriori multiparametric analysis was performed of human oocytes which failed to give pronucleated zygotes after IVF in cases of very low rates of fertilization or complete fertilization failure.

Methods: The analysis included: (i) the state of the maternal and paternal chromatin; (ii) quality of the metaphase II oocytes; and (iii) cortical granule (CG) distribution.

Results: Most oocytes were arrested in metaphase II, but they were abnormal in 50% of cases.