Improvement of covalent immobilization procedure of β-galactosidase from Kluyveromyces lactis for galactooligosaccharides production: Modeling and kinetic study.

Galactooligosaccharides (GOS) are prebiotics produced from lactose through an enzymatic reaction. Employing an immobilized enzyme may result in cost reductions; however, the changes in its kinetics due to immobilization has not been studied. This study experimentally determined the optimal reaction conditions for the production of GOS from lactose by β-galactosidase (EC 3.