[Free radical modification of proteins in brain structure of Sprague-Dawley rats and some behaviour indicators after prenatal stress].

We studied the influence of late prenatal stress on free radical oxidation processes in Sprague-Dawley rats cortex, striatum, hippocampus, hypothalamus proteins. It was shown that after prenatal stress most changes were observed in hypothalamus and hippocampus. It was shown that in hypothalamus spontaneous oxidation level increased, but level of induced oxidation decreased, the opposite changes were found in hippocampus.

[Free radical oxidation of lipids in the rat hypothalamus under stress after cortisol pretreatment].

Free radical oxidation of lipids in the rat hypothalamus after cortisol pretreatment weas investigated. Three-fold cortisol injection (25 mg/kg, once per day) resulted in significant decrease of average level of Schiffbases, the final products of lipid oxidation. Stress causes increase of average level of Schiff bases in the control animals (without cortisol pretreatment).

Free-radical lipid oxidation in the brains of active and passive rats during the development of post-stress depression.

Free-radical lipid oxidation was studied in the cerebral cortex, striatum, hippocampus, and hypothalamus of KHA and KLA rats (Koltushi High and Low Avoidance) during the development of post-stress depression. After unavoidable emotional-pain exposure, changes in the free-radical oxidation of lipids were phasic in nature and had a clear structural specificity in the early phases. During the maximum development of depression, the most marked impairments to lipid peroxidation were seen in KHA rats in the striatum and hippocampus, while the greatest changes in KLA rats were seen in the striatum and hypothalamus.

Effects of prenatal stress on free-radical lipid and protein oxidation and superoxide dismutase activity in cerebral cortex neurons and neuroglia in the rat brain.

The processes of free-radical oxidation of proteins and lipids and superoxide dismutase activity were studied in neurons and neuroglia of the cerebral cortex in rats during ontogenesis and after prenatal stress. Regardless of age, normal animals were found to have higher levels of free-radical lipid and protein oxidation in neurons than in glia. This same pattern was also observed in relation to superoxide dismutase.

[Peroxidation of lipids in the brain of active and passive rats during development of poststress depressions].

Peroxidation of lipids in a cortex of the large hemispheres, striatum, hippocampus and hypothalamus of rats of the lina KHA and KLA (Koltushi High and Low Avoidance) during development of poststress depressions was studied. After emotional painful act in initial terms of change, peroxidation of lipids had phasic character and differed in precise structural specificity. During maximal development of depression, the most expressed infringements of lipids peroxidation occurred in KHA rats in striatum and hippocampus, and at KLA rats--in the striatum and hypothalamus.

[Influence of prenatal stress on free radical oxidation lipids, both proteins and activity of superoxiddismutase in neurones and glial cells of the rat brain cortex].

Processes of free radical oxidation of protein, lipids, and activity of superoxiddismutase in neurons and glial cells of the rat brain cortex in ontogenesis and after prenatal stress. Irrespective of age, the level of free radical oxidation of lipids and proteins in neurons is higher in comparison with the glia. The same was found in the study of superoxiddismutase activity.

Free radical lipid oxidation in brain cortex neurons and neuroglia during convulsions.

The amount of lipid peroxidation products (conjugated dienes and trienes and Schiff bases) in cortical neurons of the cerebral hemispheres decreased by 30% at the peak of convulsions observed 10-15 min after intraperitoneal injection of picrotoxin. In neuroglial cells of control animals the intensity of lipid peroxidation in was 1.7-2.

Protein peroxidation in the plasma of prenatally stressed rats.

We studied the effect of prenatal stress on protein peroxidation in the plasma of rats during postnatal ontogeny. Oxidative destruction of proteins in prenatally stressed rats differed from that in control animals. These differences were most pronounced in postnatal ontogeny, i.

Lipid peroxidation in the striatum of rats during stress after administration of cortisol.

Lipid peroxidation processes were studied in the striatum during stress in conditions of prior administration of cortisol. Three doses of cortisol (25 mg/kg, daily) had no significant effect on the levels of lipid peroxidation products six days after injections ended. However, lipid peroxidation responses to stress during this period in animals given cortisol were significantly stronger than in controls (there were decreases in the intermediate products of lipid peroxidation and increases in the quantities of Schiff bases).

The use of HSP70 for prevention of consequences of unavoidable stress in rats.

We studied whether intranasal treatment with HSP70 can be used for the correction of maladaptive behavior in rats after unavoidable stress. After 3 intranasal injection of HSP70 in a dose of 3.25 microg we observed normalization of adaptive behavior after unavoidable stress and improvement of physiological reserves of the organism.

Differences in protein peroxidation in pregnant rats selected by nervous system excitability threshold.

Differences in the intensity of serum protein peroxidation were detected in pregnant rats with high and low peripheral nervous system excitability threshold in stress. Stress induced shifts in serum protein peroxidation, but these changes were opposite in rats of the two studied strains.

Serum protein peroxidation in rats selected by the rate of active avoidance conditioning under normal conditions and during stress.

Serum protein peroxidation was studied in rats with different strategy of adaptive behavior at rest and during short-term immobilization stress. Oxidative destruction of serum proteins was less intensive in KLA rats compared to that in KHA rats. Stress stimulated peroxidation of serum proteins in KHA rats.

[Lipid peroxidation in the rats striatum during stress after cortisol administration].

Lipid peroxidation in the rat striatum under stress after cortisole injection was investigated. Three days cortisole injections (25 mg/kg every day) do not affect the level of lipid peroxidation products 6 days after termination of the hormone injection. However, in these periods, cortisole injected rats had a more significant response of lipid peroxidation to stress than the control animals (decrease of intermediate products and increase of Shift bases).

[Effect of fractions of the cerebrospinal fluid from patients with drug dependence on basic electrophysiological characteristics of the rat olfactory cortex slices].

Heroin addicts at the initial stage of abstinence syndrome were subjected to detoxication by liquorosorption technique. The fractions of their cerebrospinal fluid obtained by the thin layer chromatography technique were analyzed. The substances extracted from the cerebrospinal fluid of drug addicts, presumably peptides, negatively affected the conductive function and synaptic transmission in surviving slices of the olfactory cortex of rats.

[Lipid peroxidation in the rat hippocampus after cortisol administration under stress conditions].

Cortisol administration for 3 days prior to a 6-day rest decreased formation of lipid peroxides in the rat hippocampus under stress. Exogenous cortisol limits the response of oxygen-reactive species to the lipids and exerts a regulating influence on development of a response to stress.

[ATPase activity in neurons and neuroglia during convulsions induced by picrotoxin].

Na+,K(+)-ATPase activity was studied in neurones and neuroglia under conditions of convulsions caused by picrotoxin administration. Picrotoxin is a stimulant which causes convulsions by suppression of presynaptic inhibition. Na+,K(+)-ATPase activity in neuroglia was increased in convulsion, bat was not altered in neurones.

[Seasonal changes in adrenoreactivity and a modification in the fractional phospholipid composition of the frog bladder].

The study has shown seasonal changes in the responses of the frog bladder isolated preparation to exogenous adrenalin. The finding is explained by heterogeneity of the smooth muscle adrenoreceptors and alteration of functionally domineering populations during the winter-spring cycle. The changes corresponded to modifications of the fraction composition of phospholipids, the modifications being, probably, one of the ways of regulation of the smooth muscle adrenoreceptor apparatus' activity.

[Metabolism of neuronal and neuroglial phospholipids in seizures].

Metabolism of individual phospholipids was studied in neurones and neuroglia under conditions of convulsions caused by picrotoxin administration. Metabolic activity of all the phospholipids studied was increased in neuronal membranes under extreme conditions of convulsive attacks. The rate of metabolism of phosphatidylcholine, phosphatidylethanolamine and sphingomyelin was increased 2-2.

The study of volt-ampere characteristics of ionic channels formed by gramicidin A.

A method of measurement of the non-linearity coefficient of volt-ampere characteristics of the type i(U) approximately = U(1 + beta U2) has been developed for ionic channels formed by gramicidin A, using the third harmonic of the membrane current. The shape of the volt-ampere characteristics (VA) of ionic channels formed by gramicidin A did not depend on the antibiotic concentration in the membrane. The coefficient beta of non-linearity of VA of membranes modified by gramicidin A depended on electrolyte concentration "c" and it increased proportionally with the lg c from -17 V-2 at 0.

[Influence of erythrocyte destruction kinetics on the dynamic behavior of the erythropoiesis system].

A simple mathematical model was used to analyse the influence of cell destruction kinetics on the dynamics of erythropoietic system. Two ways of erythrocyte destruction were compared: random destruction and destruction of cells at their maximum age. It has been shown that mostly used description of erythrocyte destruction as a random process results in a great stability increase (about three times in the critical feedback coefficient).

[Metabolism of different phospholipid fractions in neurons and neuroglia during myelinization].

Fractions, enriched with neurones and neuroglia, were isolated from brain cortex of 10, 20 and 30 days old rats using preparative ultracentrifugation in sucrose and Ficoil density gradient. Metabolism of phospholipids in neurones and neuroglia during the period of intensive myelinization was shown to have a number of specific properties resulting from the functional diversity of the cell populations in the central nervous system.

[Change of constant electric field in man in the course of adaptation to hypokinesia].

A study was carried out of change kinetics of man's constant electric field in the course of adaptation to hypokinesia from normal physical load and vice versa. It has been shown that adaptation, a transitional regime from one physiological state to another, involves similar values of the electric potential difference between the fixed points of human skin.

[Change of current-voltage characteristics of amphotericin B ion channels accompanying its incorporation into the membrane].

Non-linearity of amphotericin B current-voltage characteristics is determined by means of the third harmonic generated in the membrane. A change of the sign of non-linearity is revealed during incorporation of antibiotic molecules into a membrane, i. e.