Publications by authors named "Flemetakis E"

According to the Food and Agriculture Organization of the United Nations (FAO) more than 14% of the world's food production is lost every year before reaching retail, and another 17% is lost during the retail stage. The use of the expiration date as the main estimator of the life-end of food products creates unjustified food waste. Sensors capable of quantifying the effective food freshness and quality could substantially reduce food waste and enable more effective management of the food chain.

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  • The study emphasizes the significance of microbial communities in fish hatcheries for overall fish health and welfare, examining live feeds like microalgae and rotifers used in Mediterranean fish production.
  • Utilizing 16S rRNA amplicon Novaseq sequencing, researchers identified 46,745 taxonomically annotated ASVs and found that incoming environmental water significantly affects the presence of potential fish pathogens.
  • The analysis revealed a consistent yet diverse bacteriome across different hatcheries and highlighted specific opportunistic pathogenic genera, suggesting that mapping these communities can inform better hatchery management practices for improved fish health and sustainability.
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  • Microalgae, specifically Tetraselmis chuii, respond differently to various light wavelengths, affecting their growth and metabolism.
  • Different filtered light regimes resulted in lower growth rates, with blue and green filters impacting growth more than red light.
  • Transcriptomic changes were observed, like increased production of ribosome-related genes under green light and altered metabolite profiles, indicating that light filtering can be utilized for specific metabolic modifications in microalgae.
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Selenium-binding proteins (SBPs) represent a ubiquitous and conserved protein family with yet unclear biochemical and molecular functions. The importance of the human homolog has been extensively studied as it is implicated in many cancer types and other diseases. On the other hand, little is known regarding plant homologs.

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  • Bacteriophage therapy shows promise against multidrug-resistant (MDR) pathogens, but its adoption in clinical practice is slowed due to a lack of familiarity with its pharmacokinetic (PK) and pharmacodynamic (PD) properties.
  • The unique characteristics of bacteriophages, such as their ability to replicate in host bacteria and their interaction with the immune system, make conventional PK/PD estimation methods inadequate.
  • Challenges in optimizing bacteriophage therapy include understanding diverse bacteriophage types and resistance development among MDR pathogens, necessitating further research for better application against infections.
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  • Nitrogen-fixing legumes can help remediate Mercury-contaminated soil, but their effectiveness is influenced by phosphorus availability for nodule formation.
  • A study on two Robinia pseudoacacia L. strains from different climates revealed that phosphorus deficiency reduces toxicity from Mercury at the molecular level, benefiting plant health and nutrient use.
  • Rhizobia inoculation enhanced plant resilience against Mercury and phosphorus deficiency, with one strain showing better nodulation and biomass, making it a promising candidate for future phytoremediation efforts in phosphorus-poor environments.
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  • Microalgae, like Tetraselmis chuii, face oxidative stress in nature and develop specific mechanisms to adapt.
  • Exposure to 0.5-mM hydrogen peroxide (HO) severely reduced cell viability and negatively impacted photosynthetic capacity over time.
  • A rapid multi-omics response within the first hour revealed significant changes in metabolism and cellular functions, indicating both immediate and long-term impacts of oxidative stress on T. chuii.
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Biochar could reshape microbial communities, thereby altering methylmercury (MeHg) concentrations in rice rhizosphere and seeds. However, it remains unclear whether and how biochar amendment perturbs microbe-mediated MeHg production in mercury (Hg) contaminated paddy soil. Here, we used pinecone-derived biochar and its six modified biochars to reveal the disturbance.

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Interaction of different environmental constrains pose severe threats to plants that cannot be predicted from individual stress exposure. In this context, mercury (Hg), as a typical toxic and hazardous heavy metal, has recently attracted particular attention. Nitrogen (N)-fixing legumes can be used for phytoremediation of Hg accumulation, whereas N availability could greatly affect its N-fixation efficiency.

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Bacteria employ a wide range of molecular mechanisms to confer resistance to bacteriophages, and these mechanisms are continuously being discovered and characterized. However, there are instances where certain bacterial species, despite lacking these known mechanisms, can still develop bacteriophage resistance through intricate metabolic adaptation strategies, potentially involving mutations in transcriptional regulators or phage receptors. species have been particularly useful for studying the orchestrated metabolic responses of Gram-negative marine bacteria in various challenges.

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The plant microbiota can affect plant health and fitness by promoting methylmercury (MeHg) production in paddy soil. Although most well-known mercury (Hg) methylators are observed in the soil, it remains unclear how rice rhizosphere assemblages alter MeHg production. Here, we used network analyses of microbial diversity to identify bulk soil (BS), rhizosphere (RS) and root bacterial networks during rice development at Hg gradients.

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, a significant opportunistic marine pathogen, has been a challenge to the aquaculture industry, leading to severe economical and production losses. Phage therapy has been an auspicious approach in controlling such bacterial infections in the era of antimicrobial resistance. In this study, we isolated and fully characterized a novel strain-specific phage, vB_VhaS_MAG7, which infects MM46, and tested its efficacy as a therapeutic agent in challenged gilthead seabream larvae.

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The biology and biotechnology of bacteriophages have been extensively studied in recent years to explore new and environmentally friendly methods of controlling phytopathogenic bacteria. Pseudomonas syringae pv. tomato (Pst) is responsible for bacterial speck disease in tomato plants, leading to decreased yield.

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Selenium-binding proteins represent a ubiquitous protein family and recently SBP1 was described as a new stress response regulator in plants. SBP1 has been characterized as a methanethiol oxidase, however its exact role remains unclear. Moreover, in mammals, it is involved in the regulation of anti-carcinogenic growth and progression as well as reduction/oxidation modulation and detoxification.

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Atmospheric nitrogen (N)-fixing legume trees are frequently used for the restoration of depleted, degraded, and contaminated soils. However, biological N fixation (BNF) can also be performed by so-called actinorhizal plants. Actinorhizal plants include a high diversity of woody species and therefore can be applied in a broad spectrum of environments.

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The concentration of nitrogen in must is critical to yeast fermentation efficiency and wine aroma profile. The present work determined the effect of the amount of yeast assimilable nitrogen (YAN) on fermentation kinetics, aroma production, and gene expression patterns of the wine yeast . Fermentations were performed under two different YAN concentrations of must.

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The potential of renewable energy application via direct electrode interaction for the production of bio-based chemicals is a promising technology. The utilization of extracellular energy in pure culture fermentations aims in intracellular redox balance regulation in order to improve fermentation efficiency. This work evaluates the impact of a bioelectrochemical system in succinic acid fermentation and the metabolic response of Actinobacillus succinogenes.

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is an important pathogen of marine animals and has been the target of phage therapy applications in marine aquaculture for many years. Here, we report the isolation and partial characterization of a novel species of the family, the Vibrio phage Artemius. The novel phage was species-specific and could only infect strains of .

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L. has various pharmacological properties, known for over 3600 years. These properties are attributed mainly to biologically active substances, which belong to the terpenoid group and include crocins, picrocrocin and safranal.

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The production of various biochemical compounds such as proteins, glucans and glucanases, from the mycelium of four strains of Basidiomycetes species, , , and , during batch culture in shaking flasks, was studied. Fungi were cultured for 26 days in defined media with glucose as carbon source and were primarily evaluated for their ability to consume glucose and produce mycelial mass and intracellular polysaccharides (IPS). Results showed that on the 26th day of cultivation, produced the maximum biomass (16.

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Selenium-binding proteins (SBPs) represent a ubiquitous protein family implicated in various environmental stress responses, although the exact molecular and physiological role of the SBP family remains elusive. In this work, we report the identification and characterization of CrSBD1, an SBP homolog from the model microalgae Chlamydomonas reinhardtii. Growth analysis of the C.

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Although carotenoids generally possess antimicrobial and antioxidant properties, the in vivo synergistic action of carotenoid blends derived from plant-based by-products has not been thoroughly studied. Therefore, the carotenoid characterization and antimicrobial potential of extract as well as the impact of this carotenoid-rich extract (CCE) dietary supplementation on the performance, meat quality, and immune-oxidative status of broiler chickens were determined. One hundred and twenty one-day-old hatched chicks (Ross 308) were allocated to two dietary groups, with four replicate pens of 15 birds each.

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Tailed bacteriophages have been at the center of attention, not only for their ability to infect and kill pathogenic bacteria but also due to their peculiar and intriguing complex contractile tail structure. Tailed bacteriophages with contractile tails are known to have a Myoviridae morphotype and are members of the order Caudovirales. Large bacteriophages with a genome larger than 150 kbp have been studied for their ability to use multiple infection and lysis strategies to replicate more efficiently.

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