The detection of specific hypermethylated WIF1 and NPY genes in circulating DNA by crystal digital PCR™ is a powerful new tool for colorectal cancer diagnosis and screening.

Background: In oncology, liquid biopsy is of major relevance from theranostic point of view. The searching for mutations in circulating tumor DNA (ctDNA) in case of colorectal cancers (CRCs) allows the optimization of patient care. In this context, independent of mutation status biomarkers are required for its detection to confirm the presence of ctDNA in liquid biopsies.

Bictegravir/emtricitabine/tenofovir alafenamide combination in the management of kidney transplant patients with HIV receiving immunosuppressants.

We report here a drug-drug interaction with tacrolimus in a HIV-positive patient with renal transplant, after switch from highly active antiretroviral therapy with boosted protease inhibitors to the combination bictegravir/emtricitabine/tenofovir alafenamide. Although the tacrolimus doses were adapted to take account of the pharmacokinetic interactions with protease inhibitors, a tacrolimus overdosage occurred in the patient nonetheless. Through this case report, we highlight the need to consider a sufficient timeframe of withdrawal of protease inhibitors, which induce a prolonged drug-drug interaction with tacrolimus.

Real-Life Impact on Lipid Profile of a Switch from Tenofovir Disoproxil Fumarate to Tenofovir Alafenamide in HIV-Infected Patients.

Background: Tenofovir disoproxil fumarate is a prodrug of tenofovir diphosphate that exposes patients to renal toxicity over the long term. Tenofovir alafenamide, a new prodrug, now makes it possible to reduce toxicity, but at the cost of an alteration in lipid profile. There is currently no recommendation for follow-up of lipid profile when switching from tenofovir disoproxil fumarate to tenofovir alafenamide.

[The flexibility of family medicine].

We are a European academic group of family doctors and we propose a definition of flexibility in family medicine. A review of the literature shows that flexibility and complexity are emerging concepts in the field of family practice. The outcomes of a workshop at the WONCA-Europe congress in 2014 are discussed.

C-reactive protein to initiate or withhold antibiotics in acute respiratory tract infections in adults, in primary care: review.

Background: There is a discrepancy between the incidence of viral pathogens and the frequency of prescribing antibiotics for acute respiratory tract infections (ARTIs). Typically, primary care physicians have few possibilities to order sophisticated laboratory tests to help distinguish viral pathogens from bacterial pathogens. C-Reactive Protein (CRP) measurement could help the primary care physician to discriminate more easily between these two conditions, and should contribute to reducing unnecessary prescribing of antibiotics during the first patient consultation.

New polyazole derivatives from 2-(2,4-dichlorophenyl)-1,3-dioxolane. Antifungal activity. Structure-activity relationships.

Six new polyazole derivatives from 2-(2,4-dichlorophenyl)-1,3-dioxolane were studied for their antifungal activity against pathogenic fungi for humans and animals. The antimycotic effect was largely restricted to the filamentous fungi with Aspergillus fumigatus and Scedosporium apiospermum being remarkably susceptible. Three compounds were as effective as the two references, ketoconazole and oxiconazole.

Therapeutic formats in the resolution of relationship dysfunction: an acceptability investigation.

The current study examined the acceptability of four treatment-formats commonly used in marital therapy: conjoint, concurrent, group, and individual. A total of 356 subjects were randomly assigned to experimental conditions. These included three types of information (descriptive, research-oriented, and group-oriented) and two differing case histories presented across the treatment formats.

Marital therapy formats: an analysis of acceptability ratings with married spouses.

One hundred thirty-five married individuals evaluated the acceptability of alternative therapeutic formats commonly employed in marital therapy. Distressed and nondistressed spouses were randomly assigned to experimental conditions including two types of educational information (descriptive and group-oriented) presented across three treatment formats (i.e.