Administration of two antagonists of the cholecystokinin(B)/gastrin receptor does not totally inhibit the pancreatic response to a meal in the pig.

Introduction And Aims: The role of the cholecystokinin B (CCK(B))/gastrin receptor in the pancreatic response to a standard meal was investigated in the pig.

Methodology: Twenty-four pigs were prepared surgically for the collection of the pancreatic juice and an intravenous perfusion. On experimental days, the pigs were perfused with one of two CCK(B)antagonists (L-365,260 or PD 135156) or the vehicle for 2 hours.

Effect of bombesin at low doses on the secretion of the exocrine pancreas and on plasma gastrin concentration in the conscious pig.

We investigated the role of low-doses of bombesin in the regulation of exocrine secretion in the pancreas of the conscious pig. In ten growing castrated male Large White pigs, bombesin was infused intravenously for 1 h at doses of 0 to 500 pmol/kg/h under a stimulation of secretin (36 pmol/kg/h). In six pigs, bombesin (50 pmol/kg/h) was administered alone for 2 h and its effect on pancreatic secretion was compared to that of an infusion of secretin.

Physiological effects of a pea protein isolate in gnotobiotic rats: comparison with a soybean isolate and meat.

Pea proteins have been considered for the introduction into the human diet only recently. This protein source was tested on nutritional and digestive parameters in heteroxenic male Fischer rats inoculated with a human faecal microflora from a methane producer. Compared to soybean proteins, pea proteins have similar effects on the rat's endogenous and bacterial digestive patterns.

Influence of caecal microflora and of two dietary protein levels on the adaptation of the exocrine pancreas: comparative study in germ-free and conventional rats.

Dietary proteins are degraded by both endogenous enzymes and the caecal microflora. In conventional rats the enzyme content of the pancreas depends on the amount of dietary protein. The influence of the caecal microflora on this process is unknown.

Early adaptation of pancreas to a protein-enriched diet: role of cholecystokinin and gastrin-releasing peptide.

Feeding rats a diet containing high levels of protein (as casein) increases the secretion and biosynthesis of pancreatic serine proteases. Cholecystokinin (CCK) presumably plays a role in this process although other GI peptides such as the gastrin-releasing peptide (GRP) may be involved. In this article, we describe the kinetics of pancreatic adaptation to a diet containing 45% protein as soybean and fish.

Influence of diet composition on intestinal volatile fatty acid and nutrient absorption in unanesthetized pigs.

Two experiments were conducted to study the effect of the amount and nature of fiber and carbohydrates on nutrient and VFA absorption. Five Large White pigs in each crossover experiment were accustomed to a semisynthetic 14% protein diet containing 6 (LC) or 16% (HC) pure cellulose (Exp. 1) or 22% alfalfa meal (6.

Influence of meal frequency on postprandial variations in the production and absorption of volatile fatty acids in the digestive tract of conscious pigs.

Five Large White pigs of 62.2 +/- 1.4 kg mean body weight were fitted with permanent catheters in the portal vein and carotid artery and with an electromagnetic flow probe around the portal vein to study the absorption of volatile fatty acids (VFA) by measuring the concentration of these metabolites in hourly blood samples, and by determining the portal blood flow rate for a period of 12 h after intake of a single 800-g meal (6% crude fiber) preceded by 12 or 24 h of fasting.

[Effect of the nature of carbohydrates in pig diets on the intestinal absorption of volatile fatty acids].

The appearance of nutrients (amino nitrogen and reducing sugars) and microbial metabolites (volatile fatty acids, VFA) was measured quantitatively in five pigs. After ingestion of a semi-synthetic diet (RFL) containing 22% lucerne meal (6% crude fibre), the absorption of reducing sugars (RS) in the small intestine was high (97.8%) and that of VFA low (880 +/- mmoles/24 hrs.

[Influence of purified cellulose level in the diet of pigs on intestinal absorption of volatile fatty acids].

Nutrient and volatile fatty acid (VFA) absorption to portal blood was measured twice at 7 days interval in five pigs fed a semi-synthetic diet containing 6 (R6) or 16% (R16) purified cellulose for 21 or 7 days. The daily absorption (24 hrs.) of VFA in the large intestine which was larger (NS) when the cellulose level rose, increased significantly (P less than 0.

[Measurement of the appearance of volatile fatty acids in the portal vein during digestion in conscious pigs].

A method used to measure the nutrient exchanges between the intestinal lumen and the portal blood was applied to 3 pigs receiving 800 g of a diet containing 6% of cellulose. During a postprandial period of 24 hrs., the mixture of volatile fatty acids (90 mM/h) appearing in the portal vein was composed of acetic acid (57%), propionic acid (30%), butyric acid (9%), isovaleric (2.