[Investigation of the frequency and distribution of beta-lactamase genes in the clinical isolates of Acinetobacter baumannii collected from different regions of Turkey: a multicenter study].

The diversity and distribution of TEM, SHV and CTX-M type of extended-spectrum beta-lactamases (ESBLs) are important for the treatment and control of infections. Determination of ESBL genes in clinical isolates by polymerase chain reaction (PCR) and DNA sequencing can obtain useful data for their molecular epidemiology and risk. The aim of this study was to investigate the frequency of beta-lactamase genes in Acinetobacter baumannii strains isolated from different regions of Turkey.

Evaluation of Borrelia burgdorferi sensu lato seroprevalencein the province of Bolu, Turkey.

Background/aim: The aim of the present study was to determine the seroprevalence of Borrelia burgdorferi sensu lato in the city center and the province of Bolu, Turkey.

Materials And Methods: A stratified sampling method was used to determine the study population. A total of 196 blood samples were collected.

The role of human papilloma virus and herpes viruses in the etiology of nasal polyposis.

Background/aim: The aim of this study was to investigate the etiological role of human papilloma virus (HPV), herpes simplex virus (HSV), varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpes virus-6 (HHV-6) and -7 (HHV-7) in the occurrence of nasal polyposis.

Materials And Methods: Nasal polyp samples from 30 patients with nasal polyposis and normal nasal mucosa from 10 patients without nasal polyps were obtained. DNA was extracted from tissues.

Evaluation of 4 methods for the serological diagnosis of Epstein-Barr virus infection using an immunofluorescence assay as the reference method.

Background/aim: Tests specific for VCA IgM, VCA IgG, and EBNA IgG are used to diagnose Epstein-Barr virus (EBV) infections and interpret disease status. The immunofluorescence assay (IFA) is accepted as the "gold standard" test. The purpose of this study was to evaluate the performance of 4 methods in comparison with IFA.

[Carriage of class 1 and 2 integrons in Acinetobacter baumannii and Pseudomonas aeruginosa isolated from clinical specimens and a novel gene cassette array: blaOXA-11-cmlA7].

The dissemination of antibiotic resistance genes between bacteria leads to serious problems in the treatment of infectious diseases. It has been shown that resistance genes can also be carried by the integrons. There are limited studies regarding the carriage of class 1 and 2 integrons in Acinetobacter baumannii and Pseudomonas aeruginosa clinical strains in Turkey.

[In vitro activity of ceftaroline to MRSA isolates: a multicenter study].

Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging pathogen that cause severe community- and hospital-acquired infections. Studies continue on searching alternatives due to the limited number of therapeutic options in MRSA infections. Ceftaroline is a wide-spectrum new generation cephalosporin which has been begun to be used in treatment of skin and respiratory tract infections caused by MRSA.

Efficacy of daptomycin on experimental methicillin-resistant Staphylococcus aureus keratitis in rabbits.

Purpose: We aimed to compare the efficacy of topical daptomycin (DAP) with that of vancomycin (VA) in the treatment of keratitis caused by methicillin-resistant Staphylococcus aureus (MRSA).

Methods: One hundred colony-forming unit MRSA bacteria were injected intrastromally into both corneas of 28 rabbits. Sixteen hours after injection, the rabbits' eyes were treated with 1 drop of topical DAP (10 or 50 mg/mL), VA (50 mg/mL), or isotonic saline for 19 doses.

Evaluation of emm gene types, toxin gene profiles and clonal relatedness of group A streptococci.

The aim of this study is to evaluate antibiotic susceptibilities, emm gene types, toxin gene profiles and clonal relatedness of group A streptococci (GAS) isolates obtained from patients and carriers. A total of 79 clinical isolates from patients and 60 isolates from carriers were included in the study. Emm typing, toxin gene detection for speA, speB, speC, speG and smeZ genes and pulsed-field gel electrophoresis (PFGE) was performed.

Comparison of contamination in simultaneous blood cultures with clinical findings.

Blood culture, the basic method used in the diagnosis of bacteremia or sepsis, is decisive for patient's treatment. It is emerging whether the isolated microorganism is a causative agent of the infection or a contaminant. The aim of this study was to determine whether Gram-positive microorganisms, which were isolated from blood cultures simultaneously collected from two distinct veins both revealed the same species and same susceptibility profiles as they were causative agents of the bloodstream infection.

A Case of Giant Hepatic Hydatid Cyst Infected with Morganella morganii and the Literature Review.

Hydatid cyst disease is a common worldwide zoonosis. Most of the cysts are located in the liver. Abscess formation due to infection of the cyst is an important complication.

[Evaluation of reduced susceptibility to vancomycin among MRSA strains isolated from clinical specimens].

In this study, a total of 390 methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from clinical specimens between April 2004 and June 2008, in a university hospital in Zonguldak (located at Black Sea region), Turkey, were evaluated retrospectively for reduced susceptibility to vancomycin. Brain heart infusion (BHI) plates containing 4 and 6 microg/ml of vancomycin were used to screen for vancomycin intermediate S. aureus (VISA) strains.

[Detection of Entamoeba histolytica in stool specimens with the ELISA method].

In this study, carried out between November 2006 and September 2007, amebic cysts were observed in 44 (0.37%) out of a total of 1720 stool specimens which were examined by direct microscopy. Entamoeba histolytica specific antigen was investigated with ELISA in the specimens in which cysts were observed.