Publications by authors named "Fiona Mary Gribble"
Ghrelin Does Not Directly Stimulate Secretion of Glucagon-like Peptide-1.
J Clin Endocrinol Metab
January 2020
Article Synopsis
- Ghrelin, a gastrointestinal hormone, was studied for its potential effect on the secretion of GLP-1, an appetite-inhibiting hormone, both in humans and mice.
- Despite being administered to eight hypopituitary subjects and demonstrating some effects on insulin resistance, ghrelin did not significantly change GLP-1 levels in human subjects or in mouse intestines during experiments.
- Overall findings suggest that ghrelin does not directly stimulate GLP-1 secretion from L-cells in humans or mice, indicating no direct interaction between ghrelin and these cells.
GLUTag, NCI-H716, and STC-1 are cell lines that are widely used to study mechanisms underlying secretion of glucagon-like peptide-1 (GLP-1), but the extent to which they resemble native L-cells is unknown. We used validated immunoassays for 14 different hormones to analyze peptide content (lysis samples; n = 9 from different passage numbers) or peptide secretion in response to buffer (baseline), and after stimulation with 50 mM KCl or 10 mM glucose + 10 µM forskolin/3-isobutyl-1-methylxanthine (n = 6 also different passage numbers). All cell lines produced and processed proglucagon into GLP-1, GLP-2, glicentin, and oxyntomodulin in a pattern (prohormone convertase (PC)1/3 dependent) similar to that described for human gut.
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