Late-Stage Fluorination of Tyrosine Residues in Antiviral Protein Cyanovirin-N.

The applications of fluorinated molecules in chemical biology are rapidly expanding driven by the unique properties of C-F bonds, leading to increased interest in methodologies for controlled introduction of this atom. In this study, we present the first method for late-stage fluorination of tyrosine residues in proteins. Our results demonstrate that electrophilic fluorination using Selectfluor, a stable and non-toxic reagent, offers a straightforward and cost-effective method for labeling Cyanovirin-N (CVN), a 101-amino-acid lectin with effective antiviral activity.

Photoredox C(2)-Arylation of Indole- and Tryptophan-Containing Biomolecules.

We introduce a novel and straightforward methodology for photoredox arylation of an indole scaffold using aryldiazonium salts under mild and metal-free conditions. Our approach enables the regioselective and chemoselective introduction of several aryl groups to the C(2) position of indoles and tryptophan, even in competition with other amino acids. This approach extends to the late-stage functionalization of peptides and lysozyme, heralding the unprecedented arylation of tryptophan residues in wild-type proteins and offering broad utility in chemical biology.

Developing Amphetamine Certified Reference Materials: From Batch and Continuous-Flow Synthesis to Certification Protocol.

Certified reference materials (CRM) of amphetamine derivatives were produced through a simple, rapid and efficient synthesis in both batch and continuous-flow conditions, accompanied by the development of a comprehensive certification protocol for this class of substances. Our chemistry enabled the synthesis of MDA, MDMA, PMA and PMMA in two steps from safrole and estragole with overall yields of 38-61 % in 48 hours under batch conditions and 61-65 % in 65 minutes under continuous-flow conditions, followed by the development of a certification protocol for these materials through identity checking, homogeneity, stability, and characterization studies. Furthermore, as result of this work, a very pure CRM of MDA.

Natural Products for the Prevention, Treatment and Progression of Breast Cancer.

In this review, we summarize the most used natural products as useful adjuvants in BC by clarifying how these products may play a critical role in the prevention, treatment and progression of this disease. BC is the leading cancer, in terms of incidence, that affects women. The epidemiology and pathophysiology of BC were widely reported.

Discordances in Cosmology and the Violation of Slow-Roll Inflationary Dynamics.

We identify examples of single field inflationary trajectories beyond the slow-roll regime that improve the fit to Planck 2018 data compared to a baseline Λ cold dark matter model with power law form of primordial spectrum and at the same time alleviate existing tensions between different datasets in the estimate of cosmological parameters such as H_{0} and S_{8}. A damped oscillation in the first Hubble flow function-or equivalently a feature in the potential-and the corresponding localized oscillations in the primordial power spectrum partially mimic the improvement in the fit of Planck data due to A_{L} or Ω_{K}. Compared to the baseline model, this model can lead simultaneously to a larger value of H_{0} and a smaller value of S_{8}, a trend that can be enhanced when the most recent SH0ES measurement for H_{0} is combined with Planck and BICEP-Keck 2018 data.

Correction: Meneguzzo et al. Short-Term Effects of Forest Therapy on Mood States: A Pilot Study. 2021, , 9509.

The authors wish to make the following corrections to this paper [...

Short-Term Effects of Forest Therapy on Mood States: A Pilot Study.

Immersion in forest environments was shown to produce beneficial effects to human health, in particular psychophysical relaxation, leading to its growing recognition as a form of integrative medicine. However, limited evidence exists about the statistical significance of the effects and their association with external and environmental variables and personal characteristics. This experimental study aimed to substantiate the very concept of forest therapy by means of the analysis of the significance of its effects on the mood states of anxiety, depression, anger and confusion.

Joining Bits and Pieces of Reionization History.

Cosmic microwave background (CMB) temperature and polarization anisotropies from Planck have estimated a lower value of the optical depth to reionization (τ) compared to WMAP. A significant period in the reionization history would then fall within 6 View Article and Find Full Text PDF

Pest categorisation of non-EU viruses of L.

The Panel on Plant Health of EFSA conducted a pest categorisation of 17 viruses of L. that were previously classified as either non-EU or of undetermined standing in a previous opinion. These infectious agents belong to different genera and are heterogeneous in their biology.

Pest categorisation of non-EU viruses of L.

Following a request from the EU Commission, the Panel on Plant Health addressed the pest categorisation of the viruses and viroids of L. determined as being either non-EU or of undetermined standing in a previous EFSA opinion. These infectious agents belong to different genera and are heterogeneous in their biology.

Pest categorisation of non-EU viruses and viroids of L.

Following a request from the EU Commission, the Panel on Plant Health addressed the pest categorisation of the viruses and viroids of L. determined as being either non-EU or of undetermined standing in a previous EFSA opinion. These infectious agents belong to different genera and are heterogeneous in their biology.

Pest categorisation of non-EU viruses and viroids of L.

Following a request from the EU Commission, the Panel on Plant Health addressed the pest categorisation of the viruses and viroids of L. determined as being either non-EU or of undetermined standing in a previous EFSA opinion. These infectious agents belong to different genera and are heterogeneous in their biology.

Pest categorisation of non-EU viruses and viroids of Mill., Mill. and L.

Following a request from the EU Commission, the Panel on Plant Health performed a pest categorisation of 17 viruses and viroids, herein called viruses, of Mill., Mill. and L.

Pest categorisation of non-EU viruses of L.

Following a request from the EU Commission, the Panel on Plant Health addressed the pest categorisation of the viruses of L. determined as being either non-EU or of undetermined standing in a previous EFSA opinion. These infectious agents belong to different genera and are heterogeneous in their biology.

Continuous-flow synthesis of dimethyl fumarate: a powerful small molecule for the treatment of psoriasis and multiple sclerosis.

Dimethyl fumarate (DMF) is a methyl ester of fumaric acid and has recently gained attention due to its use as a pro-drug in different pharmaceutical preparations, besides the low price of the final molecule and no active patents being available for the synthesis of DMF, the prices of multiple sclerosis treatment are still high. In our continuous effort for the development of process intensification strategies towards the synthesis of active pharmaceutical ingredients, here we present our work on a cascade methodology for dimethyl fumarate synthesis in short reaction times and quantitative yields.

Breath Hydrogen as a Biomarker for Glucose Malabsorption after Roux-en-Y Gastric Bypass Surgery.

Objective: Abdominal symptoms are common after bariatric surgery, and these individuals commonly have upper gut bacterial overgrowth, a known cause of malabsorption. Breath hydrogen determination after oral glucose is a safe and inexpensive test for malabsorption. This study is designed to investigate breath hydrogen levels after oral glucose in symptomatic individuals who had undergone Roux-en-Y gastric bypass surgery.

Joint analysis of BICEP2/keck array and Planck Data.

We report the results of a joint analysis of data from BICEP2/Keck Array and Planck. BICEP2 and Keck Array have observed the same approximately 400  deg^{2} patch of sky centered on RA 0 h, Dec. -57.

Constipation in patients with thiamine deficiency after Roux-en-Y gastric bypass surgery.

Background/aims: Roux-en-Y gastric bypass surgery is the most common bariatric surgery worldwide. We have described thiamine deficiency in patients with small intestinal bacterial overgrowth after gastric bypass. We hypothesized that symptoms of thiamine deficiency are common after gastric bypass.

Enantioselective intramolecular aldehyde α-alkylation with simple olefins: direct access to homo-ene products.

A highly selective method for the synthesis of asymmetrically substituted carbocycles and heterocycles from unactivated aldehyde-olefin precursors has been achieved via enantioselective SOMO-catalysis. Addition of a catalytically generated enamine radical cation across a pendent olefin serves to establish a general asymmetric strategy toward the production of a wide range of formyl-substituted rings with alkene transposition. Conceptually, this novel mechanism allows direct access to "homo-ene"-type products.

(±)-(4aR,5R,8S,8aR)-8-(tert-Butyl-dimethyl-sil-yloxy)-2,5,8a-trimethyl-4a,5,8,8a-tetra-hydro-naphthalene-1,4-dione.

In the title compound, C19H30O3Si, both rings adopt a half-boat conformation. Overall, the mol-ecule approximates a U-shape as the cyclo-2-ene-1,4-dione and butyl-dimethyl-sil-yloxy substituents lie to the same side of the central cyclo-hexene ring; the methyl substituent lies to the other side of the mol-ecule. In the crystal, linear supra-molecular chains along the b axis are sustained by C-H⋯O inter-actions.

Cosmology and Fundamental Physics with the Euclid Satellite.

Euclid is a European Space Agency medium-class mission selected for launch in 2019 within the Cosmic Vision 2015-2025 program. The main goal of Euclid is to understand the origin of the accelerated expansion of the universe. Euclid will explore the expansion history of the universe and the evolution of cosmic structures by measuring shapes and red-shifts of galaxies as well as the distribution of clusters of galaxies over a large fraction of the sky.

Enantioselective organo-SOMO cycloadditions: a catalytic approach to complex pyrrolidines from olefins and aldehydes.

A new method to rapidly generate pyrrolidines via a SOMO-activated enantioselective (3 + 2) coupling of aldehydes and conjugated olefins has been accomplished. A radical-polar crossover mechanism is proposed wherein olefin addition to a transient enamine radical cation and oxidation of the resulting radical furnish a cationic intermediate which is vulnerable to nucleophilic addition of a tethered amine group. A range of olefins, including styrenes and dienes, are shown to provide stereochemically complex pyrrolidine products with high chemical efficiency and enantiocontrol.