Publications by authors named "Filip Varga"
Article Synopsis
- The study examines the synthesis and variation of pyrethrin, an insecticidal compound from Dalmatian pyrethrum, across 15 natural populations in the eastern Mediterranean.
- Researchers implemented a controlled field experiment to analyze pyrethrin content, aiming to attribute differences to genetic adaptations influenced by specific bioclimatic conditions.
- Results revealed significant variation in pyrethrin levels among populations, with the highest concentrations found in Budva and Podgorica, and five distinct bioclimatic groups formed based on environmental factors affecting pyrethrin composition.
Article Synopsis
- - Simple sequence repeats (SSRs) are widely used molecular markers important for conservation genetics, population studies, and genetic mapping, benefiting from advances in next-generation sequencing and bioinformatics tools for their design.
- - A new standalone pipeline has been developed to design PCR primers for microsatellite loci using multiple genomic assemblies from different species, assessing their polymorphism through in silico testing.
- - This approach eliminates the need for labor-intensive lab tests, producing efficient and promising SSR markers that can help identify interspecies hybrids, with demonstrated success across various taxonomic levels.
Article Synopsis
- The research explores the relationship between chloroplast genome lengths and the taxonomic proximity of species, based on the assumption that closely related species will have similar genome sequences.
- The study focuses on sequences from the asterid and rosid clades, finding that chloroplast length distributions are generally narrow within families and genera.
- Factors causing length variations include parasitic lifestyles, changes in the inverted repeat regions, and polyphyletic relationships, while outliers in length distributions suggest potential errors in sequence assembly.
Saprolegnia parasitica causes saprolegniosis, a disease responsible for significant economic losses in aquaculture and declines of fish populations in the wild, but the knowledge of its distribution and prevalence in the environment is limited. We developed a fast, sensitive and specific S. parasitica droplet digital PCR (ddPCR) assay and demonstrated its applicability for the detection and quantification of the pathogen in environmental samples: swab DNA collected from the host (trout skin, surface of eggs) and environmental DNA extracted from water.
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- The advancement of next-generation sequencing technology has increased the importance of bioinformatic tools used for assembling genomes and annotating their features.
- Genome annotation, especially for organelle genomes like chloroplast DNA, requires specialized tools to identify specific genes and structures.
- This study evaluated six annotation tools (Chloë, Chloroplot, GeSeq, ORG.Annotate, PGA, and Plann) for their effectiveness in identifying inverted repeats in chloroplast sequences and found that their success varies based on the imperfections in the assembled data.
Article Synopsis
- Studying the genetic and geographic structures of a species helps us understand its evolution and biodiversity in a specific region.
- Research on Salvia officinalis (common sage) using DNA analysis showed that the most genetically diverse populations are in central Balkans, with various haplotypes distributed across the region.
- The findings indicate that a major refuge for the species during the last ice age was located in the Balkans, with smaller refuges likely present throughout, and that the Apennines were colonized from the Balkans multiple times.
Article Synopsis
- Dalmatian pyrethrum is a plant species from the eastern Adriatic coast known for producing the natural insecticide pyrethrin; it has a complex genome that previously hindered the development of genetic markers.
- Utilizing advanced Illumina sequencing, researchers produced over 31,000 contigs containing microsatellite sequences, primarily focusing on di- and trinucleotide repeats, to develop functional markers.
- Ultimately, 17 microsatellite markers were created, facilitating genetic diversity analysis within natural populations and enhancing the ability to identify breeding lines and cultivars.
Article Synopsis
- * Researchers have fully characterized its chloroplast genome, which is 150,136 base pairs long, consisting of different genomic regions.
- * The chloroplast genome contains 108 genes, and phylogenetic analysis indicates that this species is closely related to others in its family, Asteraceae.
Article Synopsis
- Immortelle, a perennial plant from the Mediterranean, is significant in the perfume and cosmetic industries and shows considerable genetic diversity across its populations.
- A study using genetic markers revealed that while there's some genetic differentiation between populations of Helichrysum italicum, there’s high gene flow and two main genetic clusters exist.
- Key bioclimatic factors related to temperature and precipitation were linked to genetic variations, suggesting that understanding these adaptations is vital for conserving Mediterranean plant diversity.
Article Synopsis
- Researchers collected seeds from 10 populations of Dalmatian pyrethrum along the Adriatic coast to analyze variations in pyrethrin compounds, using a solid phase dispersion method and high-performance liquid chromatography (HPLC-DAD) for extraction and quantification.* -
- The study found significant variability in pyrethrin content, ranging from 0.10% to 1.35%, with the Pelješac population having the lowest average (0.22%) and the island populations of Mali Lošinj and Zlarin showing higher averages (0.87%).* -
- Four distinct chemotypes were identified, with chemotype P1 being the most desirable for breeding due to its superior pyrethrin content and
Article Synopsis
- - The advancements in next-generation sequencing technology and bioinformatics have made it easier and cheaper to assemble DNA sequences, especially for shorter organelle genomes like chloroplasts.
- - The increasing availability of complete chloroplast genome sequences in public databases is important for research in plant phylogenetics and biotechnology.
- - This study focused on identifying and correcting inconsistencies in publicly available chloroplast genome data, demonstrating the impacts of these inconsistencies on phylogenetic analysis using five different plant families as examples.
Three compounds with octahedral-tetrahedral Co(II) moieties of [Co( dppm )][CoX] type, where X = SCN (1), Cl (2), or I (4) have been synthesized and characterized by the X-ray structure analysis (1 and 4), and spectroscopic methods. The dc magnetic measurements show high magnetic anisotropy for octahedral centers whereas tetrahedral sites possess moderate D values. These results are confirmed by the ab initio calculations.
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