Publications by authors named "Filip Petira"
Article Synopsis
- * A study using SH-SY5Y neuronal cells found that exposure to higher concentrations of CdCl (above 50 μM) resulted in both GSH levels increasing and eventually depleting, particularly at 100 μM, causing elevated reactive oxygen species (ROS).
- * Mitochondrial impairment occurred even at lower CdCl concentrations (50 μM) after 24 hours, indicating that significant damage can arise before GSH depletion happens in these neuronal cells.
At present, nuclear condensation and fragmentation have been estimated also using Hoechst probes in fluorescence microscopy and flow cytometry. However, none of the methods used the Hoechst probes for quantitative spectrofluorometric assessment. Therefore, the aim of the present study was to develop a spectrofluorometric assay for detection of nuclear condensation and fragmentation in the intact cells.
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