Glioblastoma Multiforme: Sensitivity to Antimicrobial Peptides LL-37 and PG-1, and Their Combination with Chemotherapy for Predicting the Overall Survival of Patients.

Glioblastomas (GBMs) are the most malignant and intractable of all cancers, with an unfavorable clinical prognosis for affected patients. The objective was to analyze the sensitivity of GBM cells to the antimicrobial peptides (AMPs) cathelicidin (LL-37) and protegrin-1 (PG-1), both alone and in combination with chemotherapy, to predict overall survival (OS) in the patients. The study was conducted on 27 GBM patients treated in the neurosurgical department of the Almazov Medical Research Centre (Saint Petersburg, Russia) from 2021 to 2024.

Expression of molecular markers and synergistic anticancer effects of chemotherapy with antimicrobial peptides on glioblastoma cells.

Objective: Glioblastoma multiforme (GBM) is the most aggressive and fatal malignant primary brain tumor. The enhancement of the survival rate for glioma patients remains limited, even with the utilization of a combined treatment approach involving surgery, radiotherapy, and chemotherapy. This study was designed to assess the expression of IDH1, TP53, EGFR, Ki-67, GFAP, H3K27M, MGMT, VEGF, NOS, CD99, and ATRX in glioblastoma tissue from 11 patients.

A Simple Contactless High-Frequency Electromagnetic Sensor: Proof of Concept.

We report on the development of a very simple and inexpensive sensor device based on an inductance coil connected to a high-frequency electric field generator. The working principle of this sensor is as follows: liquid sample in a plastic tube is placed inside the inductance coil as its core and this core changes the properties of high-frequency electric current passing through the coil; these changes depend on sample conductivity, dielectric constant, magnetic properties, and capacitance. The electric signal registered after the coil represents a kind of a spectrum that can be effectively treated with chemometric tools.

Anticancer Effect of Cathelicidin LL-37, Protegrin PG-1, Nerve Growth Factor NGF, and Temozolomide: Impact on the Mitochondrial Metabolism, Clonogenic Potential, and Migration of Human U251 Glioma Cells.

Glioblastoma (GBM) is one of the most aggressive and lethal malignancy of the central nervous system. Temozolomide is the standard of care for gliomas, frequently results in resistance to drug and tumor recurrence. Therefore, further research is required for the development of effective drugs in order to guarantee specific treatments to succeed.

In Vitro Evaluation of the Cytotoxic Effect of Strains, Protegrin PG-1, Cathelicidin LL-37, Nerve Growth Factor and Chemotherapy on the C6 Glioma Cell Line.

Brain cancer treatment, where glioblastoma represents up to 50% of all CNS malignancies, is one of the most challenging calls for neurooncologists. The major driver of this study was a search for new approaches for the treatment of glioblastoma. We tested live , cathelicidin family peptides and NGF, assessing the oncolytic activity of these compounds as monotherapy or in combination with chemotherapeutics.

[Impairments of rats' behavioral reactions, concentration of corticosterone and expression of cytokine's gene under experimental postviral fatigue].

The aim of the main research was investigated of interaction of neural, endocrine and immune systems under experimental postviral fatigue, behavioral reactions, level of corticosterone, changes of IL-3 and IL-10 gene expression in rats' hypothalamus and INF-α in hypothalamus and spleen were analyzed. It has been shown the decrease of physical activity, increasing of corticosterone's level and gene expression of cytokines after injection of Poly I:C as a model of postviral fatigue. After remedication of Poly I:C increasing of physical activity was shown.

Stress-induced changes in the functional activity of the neuroendocrine system: the modulatory activity of derinat.

Changes in the functional activity of the hypothalamo-hypophyseal-adrenocortical system (HHACS) and hypothalamo-hypophyseal-gonadal system (HHGS) in conditions of different levels of stress and the possibility of correcting them using the native DNA formulation Derinat, which has immunomodulatory properties, were studied. The vectors of changes in hormonal reactions in both systems were found to be independent of the intensity of stress: there were increases in corticosterone levels and decreases in testosterone concentrations in different stress models. Intraperitoneal administration of Derinat at doses of 10 and 50 mg/kg increased HHACS and HHGS activity and reversed the stress-induced reduction in the blood testosterone concentration, which may be evidence that Derinat has stress-protecting effects.

Activity in the hypothalamo-hypophyseal-adrenocortical system on experimental induction of chronic fatigue syndrome.

Changes in the activity of the hypothalamo-hypophyseal-adrenocortical system (HHACS) were studied in an experimental model of chronic fatigue syndrome induced by i.p. administration of synthetic doublestranded RNA (polyriboinosinic:polyribocytidylic acid, Poly I:C) at a dose of 3 mg/kg.

[Stress-induced changes in functional activity of the neuro-endocrine system: modulatory action of derinat].

Changes in functional activity of HPA and HPG axes under stress influences of different intensity, and possible ways for their correction by native DNA preparation: derinat, possessing immune modulator effects, were studied. It was shown that the vector of changes in hormone's reactions of both axes did not depend on the intensity of stress influences: different models of stress increased corticosterone level and decreased testosterone level in rats' blood. Intraperitoneal injection of 10 and 50 mg/kg BW doses of Derinat to rats enhanced HPA and HPG axes activity, reversed stress-induced decrease of testosterone concentration in blood, that may indicate a stress-protective effect of derinat.

[Activity of hypotnalamic-pituitary-adrenal axis by induction of experimental chronic fatigue syndrom].

Changes in the activity of hypothalamic-pituitary adrenal (HPA) axis were investigated in experimental model of chronic fatigue syndrome (CFS) induced by intraperitoneal administration of synthetic double-stranded RNA (polyriboinosinic: polyribocytidylic acid, Poly I : C) to rats in the dose of 3 mg/kg body weight. In order to reveal functional changes in different links of the HPA axis, standard probes with intraperitoneal administration of ACTH and hydrocortisone against the background of cold stress application and Poly I : C injections were performed. A single injection of Poly I : C led to disordered HPA axis functions which was manifested by decreased sensitivity of the cells in the adrenal gland in response to ACTH, and suppression of the mechanism of negative feedback resulting in significant fall of cortisocterone concentration in standard assays with ACTH and hydrocortisone administration.