The Effect of Patient Education on Pain Level and Fear of Pain in Orthopedic Surgery: A Randomized Controlled Trial.

Purpose: Patients experience pain after surgery, an expected symptom, and a common and important care problem. The purpose of the study was to determine the effect of patient education on pain level and fear of pain in orthopedic trauma patients.

Design: The study is a randomized controlled trial research design.

Examining the risk factors of chronic pelvic pain and its effect on the quality of life in refugee and non-refugee women.

Background: This study aimed to determine the prevalence of chronic pelvic pain(CPP) in refugee and non-refugee women, determine the factors associated with CPP, and evaluate the effect of CPP on life quality.

Methods: This was a cross-sectional study conducted among 283 non-refugee and 278 refugee women in Turkey. A questionnaire including questions assessing chronic pelvic pain and related factors, World Health Organization Quality of Life Scale Short Form(WHOQOL-BREF), Depression Anxiety Stress Scale-21, were administered to the participants.

The role of intensive care nurses in cellular treatments during the COVID-19 pandemic.

Background: Today, the use of cellular therapies as an effective treatment in the field of health is increasing. In the COVID-19 pandemic or similar situations, cellular therapies may be sometimes life-saving. The COVID-19 pandemic has shown us that the training of intensive care nurses in special cases, such as cellular therapies, is insufficient.

Recombinant expression and antigenicity of two peptide families of neurotoxins from sp.

Background: Scorpion neurotoxins such as those that modify the mammalian voltage-gated sodium ion channels (Nav) are the main responsible for scorpion envenomation. Their neutralization is crucial in the production of antivenoms against scorpion stings.

Methods: In the present study, two designed genes - one that codes for a native neurotoxin from the venom of the Anatolian scorpion named Acra 4 - and another non-native toxin - named consensus scorpion toxin (SccTx) obtained from the alignment of the primary structures of the most toxic neurotoxins from the Middle Eastern and North African scorpions - were recombinantly expressed in Origami.

Relationship between nomophobia, fear of missing out, and perceived work overload in nurses in Turkey.

Purpose: This study was conducted to investigate the relationship between nomophobia, fear of missing out (FoMO), and perceived work overload (PWO) among nurses in Turkey.

Design And Methods: This descriptive study was carried out with 178 nurses. In the study, the Nomophobia Questionnaire, FoMO scale, and PWO scale were used.

An in vitro methodology for discovering broadly-neutralizing monoclonal antibodies.

Broadly-neutralizing monoclonal antibodies are of high therapeutic utility against infectious diseases caused by bacteria and viruses, as well as different types of intoxications. Snakebite envenoming is one such debilitating pathology, which is currently treated with polyclonal antibodies derived from immunized animals. For the development of novel envenoming therapies based on monoclonal antibodies with improved therapeutic benefits, new discovery approaches for broadly-neutralizing antibodies are needed.

Unity Makes Strength: Exploring Intraspecies and Interspecies Toxin Synergism between Phospholipases A and Cytotoxins.

Toxin synergism is a complex biochemical phenomenon, where different animal venom proteins interact either directly or indirectly to potentiate toxicity to a level that is above the sum of the toxicities of the individual toxins. This provides the animals possessing venoms with synergistically enhanced toxicity with a metabolic advantage, since less venom is needed to inflict potent toxic effects in prey and predators. Among the toxins that are known for interacting synergistically are cytotoxins from snake venoms, phospholipases A from snake and bee venoms, and melittin from bee venom.

Scorpion Venom: Detriments and Benefits.

Scorpion venom may cause severe medical complications and untimely death if injected into the human body. Neurotoxins are the main components of scorpion venom that are known to be responsible for the pathological manifestations of envenoming. Besides neurotoxins, a wide range of other bioactive molecules can be found in scorpion venoms.

The Mediterranean scorpion Mesobuthus gibbosus (Scorpiones, Buthidae): transcriptome analysis and organization of the genome encoding chlorotoxin-like peptides.

Background: Transcrof toxin genes of scorpion species have been published. Up to this moment, no information on the gene characterization of M. gibbosus is available.

Biological assays on the effects of Acra3 peptide from Turkish scorpion Androctonus crassicauda venom on a mouse brain tumor cell line (BC3H1) and production of specific monoclonal antibodies.

Constitutes of the venom scorpion are a rich source of low molecular mass peptides which are toxic to various organisms, including man. Androctonus crassicauda is one of the scorpions from the Southeastern Anatolia of Turkey with public health importance. This work is focused on the investigation of biological effects of Acra3 peptide from Androctonus crassicauda.

Production of an alkaline protease using Bacillus pumilus D3 without inactivation by SDS, its characterization and purification.

Abstract In this study, protease-producing capacity of Bacillus pumilus D3, isolated from hydrocarbon contaminated soil, was evaluated and optimized. Optimum growing conditions for B. pumilus D3 in terms of protease production were determined as 1% optimum inoculum size, 35 °C temperature, 11 pH and 48 h incubation time, respectively.

Molecular cloning and biochemical characterization of the first Na(+)-channel α-type toxin peptide (Acra4) from Androctonus crassicauda scorpion venom.

Due to the medical importance played in Turkey by stings of the scorpion Androctonus crassicauda, its venom has been studied with more attention. In this communication we report a new toxic peptide, named Acra4, because it is the fourth peptide completely characterized from venom of this scorpion. The peptide contains 64 amino acid residues stabilized by four disulfide bridges, with a molecular weight of 6937 Da.

Novel potassium channel blocker venom peptides from Mesobuthus gibbosus (Scorpiones: Buthidae).

In the present study, we report for the first time, the molecular, biochemical and electrophysiological characterization of the components present in the soluble venom from Mesobuthus gibbosus (Brullé, 1832). According to the epidemiological and clinical situation of scorpion envenomation cases M. gibbosus scorpion is one of the most important health-threatening species of Turkey.

Purification and cDNA cloning of a novel neurotoxic peptide (Acra3) from the scorpion Androctonus crassicauda.

Androctonus crassicauda is one of the Southeastern Anatolian scorpions of Turkey with ethno-medical and toxicological importance. Two toxic peptides (Acra1 and Acra2) were isolated and characterized from the venom of this scorpion. In this communication, the isolation of an additional toxin (Acra3) by chromatographic separations (HPLC and TSK-gel sulfopropyl) and its chemical and functional characterization is reported.

Metaphors used by in-service training nurses for in-service training nurses in Turkey.

Aim: To report a study conducted using a qualitative and quantitative study pattern with an aim to reveal the perceptions of in-service training nurses about in-service training nurses via metaphors.

Background: The in-service training nurse assumes great responsibility in the effective and efficient implementation of the educational activities in healthcare. In line with this, determining the perceptions of the in-service training nurses about in-service training nurses via metaphors and taking this into consideration in professional activities are important in terms of developing, changing, or questioning the perspectives of the in-service training nurses about their roles and developing their own training nurse identity.

Characterization of venom components from the scorpion Androctonus crassicauda of Turkey: peptides and genes.

The soluble venom from the scorpion Androctonus crassicauda was fractionated by high performance liquid chromatography. At least 44 different sub-fractions were resolved and collected for finger print mass analysis using an electrospray mass spectrometer. This analysis revealed the presence of 80 distinct molecular mass components, from which five were further characterized.

Betaine prevents loss of sialic acid residues and peroxidative injury of erythrocyte membrane in ethanol-given rats.

To evaluate chronic ethanol toxicity on erythrocyte membrane and preventive action of betaine as a methyl donor, 24 male Wistar albino rats were divided into three groups: control, ethanol and ethanol plus betaine group. Animals were fed 60 ml diet per day for two months. Rats in the ethanol group were fed ethanol 8 g/kg/day.