Nostocyclopeptides as New Inhibitors of 20S Proteasome.

Nostocyclopeptides (Ncps) are a small class of bioactive nonribosomal peptides produced solely by cyanobacteria of the genus . In the current work, six Ncps were isolated from strain CCNP1411. The bioactivity of these compounds was tested in vitro against 20S proteasome, a proteolytic complex that plays an important role in maintaining cellular proteostasis.

Luciferase reporter assay for small-molecule inhibitors of MIR92b-3p function: Screening cyanopeptolins produced by Nostoc from the Baltic Sea.

We developed a cell sensor that detects the liver cancer-specific microRNA MIR92b-3p, involved in hepatocellular carcinoma development and hepatitis C virus infection. To validate our small-molecule screen that employs a Huh7 human hepatoma cell line stably transfected with a pmirGLO vector containing dual luciferase reporters, we used i) a MIR92b-3p antisense or a MIR92b-3p mimicking agent (concentrations from 0.1 pM to 100 nM), ii) expression of XIST, a long non-coding RNA that is a cellular target of MIR92b, and iii) ectopic expression of Luc2 luciferase.

Bioactive Peptides Produced by Cyanobacteria of the Genus : A Review.

Cyanobacteria of the genus are widespread in all kinds of habitats. They occur in a free-living state or in association with other organisms. Members of this genus belong to prolific producers of bioactive metabolites, some of which have been recognized as potential therapeutic agents.

Cyanopeptolins with Trypsin and Chymotrypsin Inhibitory Activity from the Cyanobacterium CCNP1411.

Cyanopeptolins (CPs) are one of the most frequently occurring cyanobacterial peptides, many of which are inhibitors of serine proteases. Some CP variants are also acutely toxic to aquatic organisms, especially small crustaceans. In this study, thirteen CPs, including twelve new variants, were detected in the cyanobacterium CCNP1411 isolated from the Gulf of Gdańsk (southern Baltic Sea).

Chemical and Genetic Diversity of Nodularia spumigena from the Baltic Sea.

is a toxic, filamentous cyanobacterium occurring in brackish waters worldwide, yet forms extensive recurrent blooms in the Baltic Sea. produces several classes of non-ribosomal peptides (NRPs) that are active against several key metabolic enzymes. Previously, strains from geographically distant regions showed distinct NRP metabolic profiles.

Vitamin B12 deficiency as a potential cause of dementia.

There is a patient case with dementia and brain MRI massive abnormalities, probably in the course of vitamin B12 deficiency.

Thymectomy as an effective treatment in myasthenia.

The paper describes a patient case with early diagnosed myasthenia introduced, who after thymectomy and postsurgical pharmacology achieved a total remission of the disease within six years.

Diagnosis: miner's nystagmus.

We present the patient with pendular nystagmus. The condition is secondary to long-term work as an underground coal miner. The issue is worth describing because of its rare presence in contemporary either neurological or ophthalmological literature.

[Polyradicular neural syndrome in a patient with leukemic variant of malignant histiocytosis].

Malignant histiocytosis is rapidly progressive, fatal disorder characterised by systemic proliferation of abnormal histiocytic cells. We report a case of a 23-year old patient with leukaemia variant of malignant histiocytosis and nervous system involvement as polyradiculoneural syndrome. The leukaemia variant as well as nervous system involvement are extremely rare in malignant histiocytosis.

Does a spasm of vertebral canal exist?

During myelographic examination the travel of a contrast medium through the vertebral canal can be observed when it is applied by the lumbar puncture. When the vertebral canal is totally obstructed the contrast medium stops at the verge of the closing forming sometimes (in case of tumours) concave meniscus. In case of incomplete block of the canal the contrast medium flows around the obstacle.