- The bag-mediated filtration system (BMFS) was created to improve the environmental surveillance of poliovirus (PV) and complements traditional methods focused on acute flaccid paralysis to help with PV eradication efforts in Nairobi, Kenya, from April to September 2015.
- During the study, environmental samples were collected and processed using two methods: BMFS (which filtered over 3 liters) and a grab sample method (collecting 1 liter). The samples were then analyzed for poliovirus presence using various techniques, including rRT-PCR.
- Results showed that BMFS detected Sabin polioviruses more frequently than the other method, especially Sabin-like PV type 3, indicating its effectiveness for environmental monitoring, while no
This study highlights the importance of enteric virus surveillance due to their low infectious doses and long environmental persistence, using a novel bag-mediated filtration system (BMFS).
The researchers found that enteroviruses and PMMoV were present in 100% of wastewater samples collected from various sites in Kenya, while other viruses like adenovirus and norovirus were detected in most samples.
The consistent presence of these viruses indicates their potential use as contamination indicators and emphasizes the need for effective monitoring of water sources worldwide.