Blood coagulation alterations and thromboembolism in Crohn's disease.

The present study was undertaken in view of the higher incidence of thromboembolism in patients with Crohn's disease. The blood coagulation system was studied in 12 patients previously operated for Crohn's disease (8 cases of ileitis, 4 cases of colitis) and followed as out-patients. In 75% of cases, the disease was in an inactive stage.

Immunocytochemistry of serotonin-containing nerves in the human gut.

An immunocytochemical technique, using L-tryptophan enhancement and paraformaldehyde fixation, has been successfully applied for the demonstration of serotonin-containing neurons in the human enteric nervous system. Throughout the intestine, serotonin-immunoreactive nerves were mainly detected in the myenteric plexus and, to a smaller extent, in the submucous plexus, while hardly any nerve fibres were seen in the mucosa. This approach provides a useful tool for the investigation of neuropathological conditions of the gut.

Neuron specific enolase: a common marker for the endocrine cells and innervation of the gut and pancreas.

Neuron specific enolase, the most acidic isoenzyme of the glycolytic enzyme enolase, was first believed to be present exclusively in central neurons. More recently, it has been found in peripheral autonomic nerves and in a number of endocrine cells. An immunocytochemical study was carried out concerning the distribution of neuron specific enolase in the gastrointestinal tract and pancreas of humans and rats.

Immunological reactivity of native and modified alcohol dehydrogenase (ADH).

The extent to which treatment of horse liver alcohol dehydrogenase (ADH) by procedures known to disturb the Zn association induced conformation changes detectable by immunological techniques has been investigated. Treatment of ADH by sodium dodecyl sulphate or by total reduction and carboxymethylation leads to complete loss of reactivity with a rabbit immune serum against the native enzyme. After selective carboxymethylation the enzymatic activity was reduced but the preparation had the same immunological activity of the native enzyme.

Peptide-containing innervation of the human intestinal mucosa. An immunocytochemical study on whole-mount preparations.

The three-dimensional distribution of the peptide-containing innervation in the human intestinal mucosa was studied by fluorescence immunohistochemistry on whole-mount mucosal preparations. An extensive VIP-immunoreactive nerve supply was demonstrated at all levels, but was markedly increased in density in the distal intestine, where it formed a particularly rich network in close contact with the luminal epithelium. In contrast, substance P-containing nerve fibres formed a looser and evenly distributed innervation at all levels.

Mapping, quantitative distribution and origin of substance p- and VIP-containing nerves in the uvea of guinea pig eye.

VIP- and substance P-like immunoreactivities were found in considerable concentrations (VIP: 17.3 +/- 4.8 pmol/g, mean +/- SEM; substance P:11.

Peptidergic nerves.

In recent years the autonomic nervous system has been shown to consist of nerves containing a number of different neurotransmitter substances; a system far more complex than was originally thought. It has been demonstrated that a large part of the autonomic nervous system contains peptides, and that, in the gut, these nerves form a major complex that infiltrates the entire length and breadth of the tract. The detailed study of this peptidergic system has been facilitated by the development of specialised immunocytochemical methods, which have yielded information on the distribution and morphology of the various types of nerve.

Secretin-stimulated trypsin-like immunoreactivity in alcoholics.

Serum trypsin-like immunoreactivity (TLI) was studied in alcoholics without evidence of pancreatic disease and in controls. Basal values were 29 +/- 4.6 microgram/l (mean +/- S.

Effect of secretin on serum trypsin-like immunoreactivity in alcoholics.

Serum trypsin-like immunoreactivity (TLI) was studied with a newly developed radioimmunoassay in human subjects. The intravenous administration of secretin was followed by an evident increase of serum TLI significantly greater in alcoholics as compared to controls.

Apparent subunit heterogeneity of NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from spinach leaves.

NAD dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.

Serum gastrin in chronic pancreatitis.

Fasting serum gastrin and gastrin response to a protein meal were measured in a group of patients with chronic pancreatitis and in controls. No significant differences were found between the two groups of subjects. In patients with chronic pancreatitis no relation was found between gastrin release and the severity of pancreatic exocrine insufficiency.

Effect of cimetidine on trypsin-like immunoreactivity in serum.

Serum trypsin-like immunoreactivity (TLI) was studied after administration of secretin (GIH, 75 CU i.v.) in 10 alcoholics and in 3 patients with type IV hyperlipoproteinemia.

Serum immunoreactive trypsin in type IV hyperlipoproteinemia.

Serum immunoreactive trypsin (IRT) under basal conditions and after pancreatic stimulation with secretin was studied in 10 patients with type IV hyperlipoproteinemia (HLP) and in 10 control subjects. No significant difference was observed between basal values of the two groups (p = NS). The increase of serum IRT was already significant 5 minutes after secretin administration (p < 0.

Serum immunoreactive trypsin after secretin stimulation in chronic pancreatitis.

Serum immunoreactive trypsin (IRT) response to secretin injection was studied in 13 patients with chronic pancreatitis with different degrees of exocrine dysfunction and in 10 control subjects. The maximal increase of serum IRT from basal values and the integrated trypsin output (ITO) after secretin administration were significantly correlated with the output of chymotrypsin into the duodenum during caerulein-secretin infusion (p < 0.01), but not with the output of lipase nor of bicarbonate.

Subunit structure and activity of glyceraldehyde-3-phosphate dehydrogenase from spinach chloroplasts.

Glyceraldehyde-phosphate dehydrogenase (D-glyceraldehyde-3-phosphate : NADP+ oxidoreductase (phosphorylating), EC 1.2.1.