Impact of antiparasitic used in livestock: effects of ivermectin spiked sediment in Prochilodus lineatus, an inland fishery species of South America.

Ivermectin (IVM) is a widely used antiparasitic. Concerns have been raised about its environmental effects in the wetlands of Río de la Plata basin where cattle have been treated with IVM for years. This study investigated the sublethal effects of environmentally relevant IVM concentrations in sediments on the Neotropical fish Prochilodus lineatus.

Intersectional Hybrids between Darrow's Blueberry () and Lingonberry ( L.).

An initial cross of 'Johnblue' (Darrow's blueberry) × 'Red Sunset' (lingonberry) produced more than 30 true intersectional diploid hybrids as confirmed by molecular markers. The most vigorous of these hybrids was extensively evaluated. This hybrid, US 2535-A, was floriferous and morphologically intermediate to the respective parents.

Properties and Functional Analysis of Two Chorismate Mutases from Maritime Pine.

Through the shikimate pathway, a massive metabolic flux connects the central carbon metabolism with the synthesis of chorismate, the common precursor of the aromatic amino acids phenylalanine, tyrosine, and tryptophan, as well as other compounds, including salicylate or folate. The alternative metabolic channeling of chorismate involves a key branch-point, finely regulated by aromatic amino acid levels. Chorismate mutase catalyzes the conversion of chorismate to prephenate, a precursor of phenylalanine and tyrosine and thus a vast repertoire of fundamental derived compounds, such as flavonoids or lignin.

The septate junction component bark beetle is required for intestinal barrier function and homeostasis.

Age-related loss of intestinal barrier function has been documented across species, but the causes remain unknown. The intestinal barrier is maintained by tight junctions (TJs) in mammals and septate junctions (SJs) in insects. Specialized TJs/SJs, called tricellular junctions (TCJs), are located at the nexus of three adjacent cells, and we have shown that aging results in changes to TCJs in intestines of adult .

Connections among Land Use, Water Quality, Biodiversity of Aquatic Invertebrates, and Fish Behavior in Amazon Rivers.

Rivers in the Amazon have among the greatest biodiversity in the world. The Xingu River, one of the tributaries of the Amazon River, has a length of 1640 km, draining 510,000 km2 in one of the most protected regions on the planet. The Middle Xingu region in Brazil has been highly impacted by mining and livestock farming, leading to habitat fragmentation due to altered water quality.

Oxidative stress responses after exposure to triclosan sublethal concentrations: an integrated biomarker approach with a native () and a model fish species ().

Triclosan (TCS) is a synthetic broad-spectrum antimicrobial agent commonly used world-wide in a range of personal care and sanitizing products detected frequently in aquatic ecosystems. The aim of this study was to examine biochemical markers responses triggered by TCS in and in a native South American fish species (). Further, an integrated approach comparing both test fish species was undertaken.

Deregulation of phenylalanine biosynthesis evolved with the emergence of vascular plants.

Phenylalanine (Phe) is the precursor of essential secondary products in plants. Here we show that a key, rate-limiting step in Phe biosynthesis, which is catalyzed by arogenate dehydratase, experienced feedback de-regulation during evolution. Enzymes from microorganisms and type-I ADTs from plants are strongly feedback-inhibited by Phe, while type-II isoforms remain active at high levels of Phe.

Multibiomarker responses in Danio rerio after exposure to sediment spiked with triclosan.

Triclosan (TCS) is an antimicrobial and antimycotic agent widely used in personal care products. In aquatic environments, both TCS and its biomethylated more persistent form, methyl-triclosan (MeTCS), are usually detected in wastewater effluents and rivers, where are commonly adsorbed to suspended solids and sediments. The aim of this study was to evaluate biochemical and physiological effects in Danio rerio after a short term (2 days) and prolonged (21 days) exposures to sediment spiked with TCS acting as the source of the pollutant in the assay.

Ivermectin: A multilevel approach to evaluate effects in Prochilodus lineatus (Valenciennes, 1836) (Characiformes, Prochilodontidae), an inland fishery species.

Ivermectin (IVM) is one of the most widely used antiparasitics worldwide. It is a potent and effective drug for treatment and prevention of internal and external parasitic infections of livestock and humans. IVM is excreted unchanged in manure of treated animals.

Multibiomarker responses and bioaccumulation of fipronil in Prochilodus lineatus exposed to spiked sediments: Oxidative stress and antioxidant defenses.

Fipronil is a current use pesticide, widely used in many crops, commonly adsorbed to sediments of aquatic environments. The purpose of this study was to evaluate the biomarker responses and fipronil distribution pattern in different matrixes (fish, sediment and water) after juveniles P. lineatus exposure at two environmental concentrations (5.

Special Issue Editorial: Plant Nitrogen Assimilation and Metabolism.

Nitrogen is an important macronutrient for plant growth and development. Research has long been carried out to elucidate the mechanisms involved in nitrogen uptake, assimilation, and utilization in plants. However, despite recent advances, many of these mechanisms still are not fully understood.

Nanosilver and Silver Nitrate Toxicity in Ex Vivo-Exposed Gills of Fish and Mitigation by Humic Acids.

Silver nanoparticles (AgNP) are unique because of their biocide properties. Once released to environment, AgNP interact with the natural organic matter which impact on their fate, dispersion, and ultimate toxicity. We carried out an ex vivo exposure of gill of Corydoras paleatus fish to 100 µg L of AgNP or AgNO, alone and in combination with 10 mg L of humic acids (HA), with the aim to evaluate the potential mitigation of HA on AgNP toxic effects.

Exposure to environmental concentrations of fipronil induces biochemical changes on a neotropical freshwater fish.

Fipronil is a broad-use insecticide with severe toxicity to fish. Biomarkers responses and bioaccumulation were evaluated on Prochilodus lineatus after exposure to environmentally relevant concentrations of fipronil (0.5 μg L, 9 μg L, and 100 μg L) in a prolonged flow-through assay and ex vivo gills short-term exposition.

3D Human Pose, Shape and Texture From Low-Resolution Images and Videos.

3D human pose and shape estimation from monocular images has been an active research area in computer vision. Existing deep learning methods for this task rely on high-resolution input, which however, is not always available in many scenarios such as video surveillance and sports broadcasting. Two common approaches to deal with low-resolution images are applying super-resolution techniques to the input, which may result in unpleasant artifacts, or simply training one model for each resolution, which is impractical in many realistic applications.

Mitigation of silver nanoparticle toxicity by humic acids in gills of Piaractus mesopotamicus fish.

Silver nanoparticles (AgNPs) are one of the most produced nanoproducts due to their unique biocide properties. The natural organic matter has an important impact on nanoparticle's dispersion as it may alter their fate and transport, as well as their bioavailability and toxicity. Therefore, this study aimed to evaluate the mitigatory effect of humic acids (HAs) on AgNP toxicity.

Parkinson's Disease Tremor Detection in the Wild Using Wearable Accelerometers.

Continuous in-home monitoring of Parkinson's Disease (PD) symptoms might allow improvements in assessment of disease progression and treatment effects. As a first step towards this goal, we evaluate the feasibility of a wrist-worn wearable accelerometer system to detect PD tremor in the wild (uncontrolled scenarios). We evaluate the performance of several feature sets and classification algorithms for robust PD tremor detection in laboratory and wild settings.

SelfPose: 3D Egocentric Pose Estimation From a Headset Mounted Camera.

We present a new solution to egocentric 3D body pose estimation from monocular images captured from a downward looking fish-eye camera installed on the rim of a head mounted virtual reality device. This unusual viewpoint leads to images with unique visual appearance, characterized by severe self-occlusions and strong perspective distortions that result in a drastic difference in resolution between lower and upper body. We propose a new encoder-decoder architecture with a novel multi-branch decoder designed specifically to account for the varying uncertainty in 2D joint locations.

Comparing laboratory and in-the-wild data for continuous Parkinson's Disease tremor detection.

Passive, continuous monitoring of Parkinson's Disease (PD) symptoms in the wild (i.e., in home environments) could improve disease management, thereby improving a patient's quality of life.

Enzymes Involved in the Biosynthesis of Arginine from Ornithine in Maritime Pine ( Ait.).

The amino acids arginine and ornithine are the precursors of a wide range of nitrogenous compounds in all living organisms. The metabolic conversion of ornithine into arginine is catalyzed by the sequential activities of the enzymes ornithine transcarbamylase (OTC), argininosuccinate synthetase (ASSY) and argininosuccinate lyase (ASL). Because of their roles in the urea cycle, these enzymes have been purified and extensively studied in a variety of animal models.

Structural and Functional Characteristics of Two Molecular Variants of the Nitrogen Sensor PII in Maritime Pine.

High levels of nitrogen are stored as arginine during the last stages of seed formation in maritime pine ( Aiton). The protein sensor PII regulates the feedback inhibition of arginine biosynthesis through interaction with the key enzyme -acetylglutamate kinase (NAGK). In this study, the structural and functional characteristics of PII have been investigated in maritime pine to get insights into the regulation of arginine metabolism.

Transcriptional analysis of arogenate dehydratase genes identifies a link between phenylalanine biosynthesis and lignin biosynthesis.

Biogenesis of the secondary cell wall in trees involves the massive biosynthesis of the phenylalanine-derived polymer lignin. Arogenate dehydratase (ADT) catalyzes the last, and rate-limiting, step of the main pathway for phenylalanine biosynthesis. In this study, we found that transcript levels for several members of the large ADT gene family, including ADT-A and ADT-D, were enhanced in compression wood of maritime pine, a xylem tissue enriched in lignin.

Weakly-Supervised Learning of Category-Specific 3D Object Shapes.

Category-specific 3D object shape models have greatly boosted the recent advances in object detection, recognition and segmentation. However, even the most advanced approach for learning 3D object shapes still requires heavy manual annotations on large-scale 2D images. Such annotations include object categories, object keypoints, and figure-ground segmentation for the instances in each image.

Characteristics, quality of life and control of respiratory allergic diseases caused by house dust mites (HDMs) in Spain: a cross-sectional study.

This multicentre, cross-sectional study conducted in Spain assessed the clinical characteristics and quality of life of patients who were aged 14-55 years and had allergic rhinitis and/or asthma, which was due to house dust mite sensitisation, for at least 2 years. Overall, 296 patients were included; 60% had allergic rhinitis (mostly persistent moderate-to-severe) and 40% had rhinitis and asthma (mostly intermittent or mild-to-persistent). Patients with rhinitis had moderately reduced quality of life, which was significantly worse in adults than adolescents.

Learning Facial Action Units with Spatiotemporal Cues and Multi-label Sampling.

Facial action units (AUs) may be represented , and in terms of their . Previous research focuses on one or another of these aspects or addresses them disjointly. We propose a hybrid network architecture that jointly models spatial and temporal representations and their correlation.

Nitrogen Metabolism and Biomass Production in Forest Trees.

Low nitrogen (N) availability is a major limiting factor for tree growth and development. N uptake, assimilation, storage and remobilization are key processes in the economy of this essential nutrient, and its efficient metabolic use largely determines vascular development, tree productivity and biomass production. Recently, advances have been made that improve our knowledge about the molecular regulation of acquisition, assimilation and internal recycling of N in forest trees.