Influence of preliminary etching on the stability of bonds created by one-step self-etch bonding systems.

We evaluated the effects of preliminary etching of dentine on the stability of the bond created by one-step self-etch adhesives under different storage conditions. Adper Easy Bond (3M ESPE) and iBond Self-Etch (iBond SE; Heraeus Kulzer) were applied with an etch-and-rinse (i.e.

Effects of pH, ionic strength, and applied voltage on migration of dental monomers in an organic matrix.

Objectives: The application of an electric field has been shown to positively influence the bonding of dentin bonding systems (DBS) by improving adhesive impregnation into dentin. However, the mechanism responsible for this phenomenon has not been completely elucidated. The aim of this study was to clarify the effects of pH, matrix ionic strength, and applied voltage on the migration of commonly used DBS monomers in a model matrix (agarose gel).

Immunohistochemical and biochemical assay of MMP-3 in human dentine.

Objective: The function of endogenous MMP-3 and its distribution within the human dentine is unclear. Thus, the aim of the present study was to assay the presence and distribution of MMP-3 within human sound dentine by means of biochemical and immunohistochemical assays.

Methods: Powdered dentine from extracted human teeth was prepared and (1) partially demineralised with 1% H(3)PO(4) for 10min or (2) untreated (control).

Role of preliminary etching for one-step self-etch adhesives.

The aim of this study was to analyse the effect of preliminary phosphoric acid etching of enamel and dentine before the application of two, one-step self-etch adhesive systems. The systems were applied onto acid-etched or smear-layer-covered enamel and dentine. The treatment groups were as follows: group 1, Adper Easy Bond (3M ESPE) on etched substrate; group 2, Adper Easy Bond (control); group 3, iBond Self-Etch (Heraeus Kulzer) on etched substrate; and group 4, iBond Self-Etch (control).

Use of a specific MMP-inhibitor (galardin) for preservation of hybrid layer.

Objective: Dentinal MMPs have been claimed to contribute to the auto-degradation of collagen fibrils within incompletely resin-infiltrated hybrid layers and their inhibition may, therefore, slow the degradation of hybrid layer. This study aimed to determine the contribution of a synthetic MMPs inhibitor (galardin) to the proteolytic activity of dentinal MMPs and to the morphological and mechanical features of hybrid layers after aging.

Methods: Dentin powder obtained from human molars was treated with galardin or chlorhexidine digluconate and zymographically analyzed.

Final rinse optimization: influence of different agitation protocols.

Introduction: This study examined the effect of different root canal irrigant agitation protocols in the penetration of an endodontic irrigant into dentinal tubules.

Methods: Fifty-six human single-rooted teeth were shaped with nickel-titanium instruments, and a final rinse of 5% sodium hypochlorite labeled with 0.2% alizarin red was performed.

Chlorhexidine stabilizes the adhesive interface: a 2-year in vitro study.

Objectives: This study evaluated the role of endogenous dentin MMPs in auto-degradation of collagen fibrils within adhesive-bonded interfaces. The null hypotheses tested were that adhesive blends or chlorhexidine digluconate (CHX) application does not modify dentin MMPs activity and that CHX used as therapeutic primer does not improve the stability of adhesive interfaces over time.

Methods: Zymograms of protein extracts from human dentin powder incubated with Adper Scotchbond 1XT (SB1XT) on untreated or 0.

Analysis of differential artificial ageing of the adhesive interface produced by a two-step etch-and-rinse adhesive.

This study was performed to evaluate the effects of different in vitro ageing techniques on the dentine-bonded interface produced by a two-step etch-and-rinse adhesive. Composite build-ups were bonded to sectioned human molars using XP BOND and cut into non-trimmed dentine-composite beams for microtensile testing. Beams were assigned to one of the following storage conditions: (i) artificial saliva, 24 h (control); (ii) 10% sodium hypochlorite (NaOCl), 1 h; (iii) 10% NaOCl, 3 h; (iv) 60,000 thermal cycles, 2 months; (v) artificial saliva, 2 months; (vi) 60,000 thermal cycles, 6 months; and (vii) artificial saliva, 6 months.

Adhesion of a two-step etch-and-rinse adhesive on collagen-depleted dentin.

Purpose: To evaluate microtensile bond strength and interfacial nanoleakage expression of adhesive interfaces created by XP-Bond on human deproteinized dentin immediately after bonding and after 6 months of artificial aging.

Materials And Methods: Noncarious human molars were selected, middle/deep dentin substrates were exposed, and either assigned to group 1 (XP-Bond applied on collagen-depleted dentin) or to group 2 (XP-Bond applied in accordance with manufacturers' instructions). In group 1, the etched dentin surface was treated with 10% NaOCl for 60 s to remove the exposed demineralized organic matrix before XP-Bond application.