Publications by authors named "Fernanda Campos Rosetti Lessa"

Introduction: Evidence regarding the impact of early loss of primary molars (ELPM) on Oral Health-Related Quality of Life (OHRQoL) is lacking. The aim of the present study was evaluating the impact of ELPM on OHRQoL of Brazilian schoolchildren aged 6-10 years.

Methods: This observational prospective cohort study was conducted with 163 schoolchildren, assigned in the primary tooth loss group (PTLG), in which all tooth loss was caused by carious lesions, and in a control group without tooth loss (CG).

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Premature loss of primary teeth can lead to negative consequences in both dentitions. The purpose of this study was to determine the factors associated with the premature loss of primary teeth and the child's socioeconomic status, main caretaker during the day, age range, sex, and previous caries experience. A total of 192 two- to 10-year-old Brazilian schoolchildren, seen at the Vila Velha University pediatric dental clinic, were assigned to two groups: the primary tooth loss group (PTLG) and a control group without tooth loss (CG)-from May 2016 to March 2017.

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Article Synopsis
  • The study aimed to assess the antibacterial effects of various chlorhexidine (CHX) concentrations against the bacteria Streptococcus mutans using two different testing methods: direct application (agar-diffusion) and indirect application (transdentinal diffusion with human dentin discs).
  • The researchers prepared Petri dishes with S. mutans and tested different CHX concentrations (0.12%, 0.2%, 1%, 2%), phosphoric acid, and control groups, measuring the zones of bacterial growth inhibition after incubation.
  • Results showed that higher CHX concentrations had greater antibacterial activity, with the most significant inhibition observed using 2% CHX on thinner dentin discs, indicating the effectiveness of CHX against S.
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Unlabelled: This in vitro study evaluated the cytotoxicity of an experimental restorative composite resin subjected to different light-curing regimens.

Methods: Forty round-shaped specimens were prepared and randomly assigned to four experimental groups (n=10), as follows: in Group 1, no light-curing; in Groups 2, 3 and 4, the composite resin specimens were light-cured for 20, 40 or 60 s, respectively. In Group 5, filter paper discs soaked in 5 µL PBS were used as negative controls.

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Objectives: To evaluate the cytotoxic effects of a bleaching agent composed of 0.01% carbamide peroxide (CP; 2.21mug/ml H(2)O(2)) on the MDPC-23 odontoblastic cell line, and to determine whether sodium ascorbate (SA) is capable of reducing, or even eliminating, the toxic effects caused by this bleaching agent.

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This study evaluated the cytotoxic effects of 2 mineral trioxide aggregate (MTA) cements - White-MTA-Angelus and a new formulation, MTA-Bio - on odontoblast-like cell (MDPC-23) cultures. Twenty-four disc-shaped (2 mm diameter x 2 mm thick) specimens were fabricated from each material and immersed individually in wells containing 1 mL of DMEM culture medium for either 24 h or 7 days to obtain extracts, giving rise to 4 groups of 12 specimens each: G1 - White-MTA/24 h; G2 - White-MTA/7 days; G3 - MTA-Bio/24 h; and G4 - MTA-Bio/7 days. Plain culture medium (DMEM) was used as a negative control (G5).

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Unlabelled: Chlorhexidine gluconate (CHX) is recommended for a number of clinical procedures and it has been pointed out as a potential cavity cleanser to be applied before adhesive restoration of dental cavities.

Objective: As CHX may diffuse through the dentinal tubules to reach a monolayer of odontoblasts that underlies the dentin substrate, this study evaluated the cytotoxic effects of different concentrations of CHX on cultured odontoblast-like cells (MDPC-23).

Material And Methods: Cells were cultured and exposed to CHX solutions at concentrations of 0.

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Objectives: The objectives of this study were to evaluate the transdentinal cytotoxicity of 10% and 16% carbamide peroxide gel (CP), as well as the ability of the antioxidant, 10% sodium ascorbate (SA), to protect the odontoblasts in culture.

Study Design: Human dentin discs of 0.5-mm thickness were obtained and were placed into artificial pulp chambers.

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It has been demonstrated that chlorhexidine digluconate (CHX) is capable of eliminating bacteria that may remain lodged in dentin after mechanical caries removal. In addition, the use of CHX on acid-etched dentin before adhesive system application delays the resin-dentin interface degradation, maintaining the integrity of the adhesive restorations. Despite these advantages of using CHX in restorative dentistry, when applied on dentin, this chemical agent may diffuse across dentinal tubules to cause toxic effects to the pulp cells.

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Objective: This study evaluated transenamel and transdentinal cytotoxic effects of a bleaching gel on the MDPC-23 cell line.

Study Design: Discs obtained from bovine incisors were placed in a metallic device to simulate an in vivo pulp chamber. Groups were formed according to the enamel surface treatment: G1: 35% H(2)O(2) bleaching gel; G2: 35% H(2)O(2) bleaching gel + halogen light; G3: halogen light; and G4: control.

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Purpose: To evaluate the cytotoxic effects of resin-based light-cured liners on culture of pulp cells.

Methods: Discs measuring 4 mm in diameter and 2 mm thick were fabricated from TheraCal (TCMTA), Vitrebond (VIT), and Ultrablend Plus (UBP). These specimens were immersed in serum-free culture medium (DMEM) for 24 hours or 7 days to produce the extracts.

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This study evaluated the cytotoxic effects of a carbamide peroxide (CP) bleaching gel at different concentrations on odontoblast-like cells. Immortalized cells of the MDPC-23 cell line (30,000 cells/cm(2)) were incubated for 48 h. The bleaching gel was diluted in DMEM culture medium originating extracts with different CP concentrations.

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Purpose: The purpose of this study was to evaluate in vitro the influence of Er:YAG laser irradiation distance on the shear bond strength of an adhesive restorative system to primary enamel.

Methods: Fifty buccal surfaces of extracted human canines were ground and divided into 5 groups (N=10). The control group was etched with 35% phosphoric acid (CA).

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Introduction: This randomized clinical trial assessed, by using microbial culture and scanning electron microscopy (SEM), the contamination by mutans streptococci (MS) colonies/biofilms on acrylic baseplates of removable orthodontic appliances and evaluated the efficacy of antimicrobial sprays (Periogard [Colgate-Palmolive Ind. Brasileira, Osasco, SP, Brazil], Cepacol [Merrell Lepetit Farmacêutica e Industrial Ltda, Santo Amaro, SP, Brazil], and sterile tap water [control]) on their disinfection.

Methods: Seventeen children were randomly enrolled in a 3-stage changeover system with a 1-week interval between each stage.

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Purpose: The purpose of this study was to investigate the shear bond strength of 2 resin-based pit-and-fissure sealants--Clinpro and Fluroshield--to saliva-contaminated and noncontaminated enamel.

Methods: Forty buccal halves of permanent molar crowns were individually embedded in polyester resin and ground with wet silicone carbide papers to obtain flat enamel surfaces. The specimens were randomly assigned to 2 groups: (A) without contamination; and (B) contaminated with 0.

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Introduction: Atresia of the maxilla is a transverse skeletal dysplasia, possibly associated with respiratory problems. For its correction, rapid maxillary expansion is a feasible orthodontic process.

Objective: To evaluate the effect of rapid maxillary expansion on the nasal cavity by acoustic rhinometry and computed rhinomanometry.

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Cri du Chat Syndrome occurs as a result of a partial deletion in the short arm of chromosome 5. Among the consequent abnormalities are low birth weight, a striking catlike cry in infancy, mental retardation, epicanthal folds, hypertelorism and dental malocclusions. This paper presents a case report on the dental treatment of a 23-year-old patient who received multidisciplinary treatment, including special education and precocious stimulation for carriers of this syndrome.

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Aim: The aim of this study was to evaluate the differences in facial proportions of nose and mouth breathing children using cephalometric analysis.

Study Design: Transversal cohort.

Material And Method: Sixty cephalometric radiographs from pediatric patients aged 6 to 10 years were used.

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