Evidence of transfer of miRNAs from the diet to the blood still inconclusive.

MicroRNAs (miRNAs) are short, non-coding, single-strand RNA molecules that act as regulators of gene expression in plants and animals. In 2012, the first evidence was found that plant miRNAs could enter the bloodstream through the digestive tract. Since then, there has been an ongoing discussion about whether miRNAs from the diet are transferred to blood, accumulate in tissues, and regulate gene expression.

Web-based tools for microRNAs involved in human cancer.

MicroRNAs (miRNAs/miRs) are a family of small, endogenous and evolutionarily-conserved non-coding RNAs that are involved in the regulation of several cellular and functional processes. miRNAs can act as oncogenes or tumor suppressors in all types of cancer, and could be used as prognostic and diagnostic biomarkers. Databases and computational algorithms are behind the majority of the research performed on miRNAs.

Circulating microRNA expression profile in B-cell acute lymphoblastic leukemia.

Background: Acute lymphoblastic leukemia (ALL) is a highly diverse disease characterized by cytogenetic and molecular abnormalities, including altered microRNA (miRNA) expression signatures.

Aim: We perform and validate a plasma miRNA expression profiling to identify potential miRNA involved in leukemogenesis

Methods: MiRNA expression profiling assay was realized in 39 B-ALL and 7 normal control plasma samples using TaqMan Low Density Array (TLDA) plates on Applied Biosystems 7900 HT Fast Real-Time PCR System. MiRNA validation was done for six miRNA differentially expressed by quantitative real-time PCR.

Use of serum-circulating miRNA profiling for the identification of breast cancer biomarkers.

MicroRNAs (miRNAs) are important regulatory molecules involved in disease pathogenesis. miRNAs are very stable in bodily fluids and can be detected in serum, plasma, saliva, and urine, among other fluids. Several studies have demonstrated the usefulness of serum miRNAs as potential biomarkers for detecting and monitoring cancer progression.

Identification and characterization of microRNAs from Entamoeba histolytica HM1-IMSS.

Background: Entamoeba histolytica is the causative agent of amebiasis, a disease that is a major source of morbidity and mortality in the developing world. MicroRNAs (miRNAs) are a large group of non-coding RNAs that play important roles in regulating gene expression and protein translation in animals. Genome-wide identification of miRNAs is a critical step to facilitating our understanding of genome organization, genome biology, evolution, and post-transcriptional regulation.

Serum circulating microRNA profiling for identification of potential breast cancer biomarkers.

MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules that can regulate gene expression, thereby affecting crucial processes in cancer development. miRNAs offer great potential as biomarkers for cancer detection because of their remarkable stability in blood and their characteristic expression in different diseases. We investigated whether quantitative RT-PCR miRNA profiling on serum could discriminate between breast cancer patients and healthy controls.

Differential expression of miR-21, miR-125b and miR-191 in breast cancer tissue.

Aims: To develop new biomarkers for early detection and to inform effective clinical management of breast cancer.

Methods: Real-time polymerase chain reaction was used to profile microRNA (miRNA) in tumor tissue from 50 breast cancer patients using non-tumor breast tissue from each patient as a control. We have focussed on three miRNA; miR-21, miR-125b and miR-191, all of which have been implicated in breast cancer with either proven or predicted target genes involved in critical cancer-associated cellular pathways.