Conjunctival vaccination of young goats with Brucella melitensis strain Rev 1.

Three groups each of 15 four-month old female kids were vaccinated with Brucella melitensis strain Rev 1 either conjunctivally (1.1 x 10(7) or 1.1 x 10(9) CFU) or subcutaneously (1.

Some aspects of experimental bovine brucellosis.

In the course of eight successive Brucella abortus experiments, 394 cows were followed by clinical, serological and bacteriological examinations. Cultures were made from vaginal swabs and colostrum at delivery and from 10 pools of lymph nodes or organs sampled at slaughter, about 6 weeks after calving. From these cows, 346 had been experimentally challenged with 1.

Vaccination with a Listeria strain of reduced virulence against experimental Listeria abortion in goats.

A live Listeria monocytogenes strain of reduced virulence, strain Aer, obtained by three successive mutations in regard to streptomycin and erythromycin, was used as vaccine on goats in two successive experiments. Animals were vaccinated either once or twice by subcutaneous route with doses varying from 6 x 10(8) to 1.5 x 10(10) CFU.

Vaccination against Listeria infection in mice with a mutant strain of reduced virulence.

Killed Listeria monocytogenes (L.m.) or living Listeria innocua, being unable to afford noticeable immunity against L.

Selection and characterization of a Listeria monocytogenes vaccinal strain of reduced virulence for mice.

In an attempt to reproduce resistance to Listeria monocytogenes induced by primo-infection, a vaccinal strain of low virulence capable of affording a good immunity was looked for. After a double mutation in regard to streptomycin, i.e.

Vaccination of ewes by a single conjunctival administration of Brucella melitensis Rev. 1 vaccine.

In a lot of 99 ewes, the efficacy of a single vaccination by the conjunctival route (VC) with either 1.4 X 10(8) or 1.4 X 10(9) CFU Brucella melitensis Rev.

[Biological control of the brucellin INRA in sensitized guinea pigs: a guide for the statistical analysis of results].

Brucellin INRA is a new allergen used for the screening of brucellosis. It shows, by a delayed-type skin hypersensitivity reaction, the sensitization induced in animals by a brucella infection. The biological potency of each batch must be compared to that of a standard allergen.

[Listeria strains isolated from sick animals and consumed silage].

Forty-eight strains of listeria isolated from sick animals (27 goats, 19 sheep, 2 cows) from 33 farms, and 40 isolated from the silage eaten by these animals were studied. Listeria were isolated more frequently from poor quality silages than from those of good quality, although they were found in 11 out of 31 excellent silages with a pH value comprised between 3.6 and 4.

[Effect of routes and doses of Salmonella abortus ovis inoculation on the abortive effect and serologic response of ewes].

Three successive experiments using a restricted number of Préalpes X Lacaune ewes were designed to form a procedure allowing the reproduction of an abortive infection with Salmonella abortus ovis. Effects of inoculations at mid-gestation were estimated by the duration of gestation, bacteriological excretion at lambing and evolutions of rectal temperatures and of serological antibodies. Serological responses were revealed with four tests: O or H agglutination tests in tubes, H agglutination or complement fixation tests in microtechnics.

Comparison between subcutaneous and conjunctival route of vaccination with Rev. 1 strain against Brucella melitensis infection in ewes.

In areas where the prevalence of Brucella melitensis infection in sheep is high, control of the disease cannot be led without the help of vaccination. The use of Brucella melitensis strain Rev. 1 vaccine affords a very good protection.

Efficacy of Brucella melitensis Rev. 1 vaccine against Brucella ovis infection in rams.

The efficacy of Brucella melitensis strain Rev. 1 vaccine against a Brucella ovis experimental infection was judged on 30 rams, 15 of which had received 8.5 X 10(8) Rev.

Congenital brucellosis in cattle associated with localisation in a hygroma.

A heifer born to a cow experimentally infected with Brucella abortus strain 544 and reared in isolation from its birth was found infected. Antibodies appeared when it was 10 months old. The heifer calved normally.

[Allergic diagnosis of bovine brucellosis. 2. Use of the allergic test in infected herds (author's transl)].

Four hundred and eighty-one cows, in 27 herds, were injected intradermally with a brucella purified protein allergen in order to study the value of the skin test in the diagnosis of bovine brucellosis. The serology was done on the serum or plasma from 444 of these cows. Two hundred and nine cows showed an allergic reaction and 174 were positive in serology.

[Allergic diagnosis of bovine brucellosis. 1. Conditions for the use of a purified protein allergen: brucellin (author's transl)].

A protein allergen extracted from the rough strain Brucella melitensis B 115, prepared for the diagnosis of Brucellosis, has been evaluated in cows. Injected in non infected and non vaccinated cows, this allergen does not give any reaction, does not sensitize for subsequent injections, does not give rise to antibodies detected by routine serologic tests, and thus does not interfere with usual screening. In cows, sensitized by injections of H 38 or 45/20 vaccines, the intradermal injection produces a delayed hypersensitivity reaction.

Vaccination against bovine brucellosis with a low dose of strain 19 administered by the conjunctival route. III. -- Serological response and immunity in the pregnant cow.

Seventy-four heifers, 7 to 12 months old, were randomized in four groups: group A, 8 heifers as controls; group B, 19 heifers vaccinated subcutaneously with 9 X 10(10) Brucella abortus strain B 19; group C, 19 heifers vaccinated as in group B, then revaccinated by the conjunctival route 6 to 8 months later with 5 X 10(9) bacteria; group D, 28 heifers vaccinated twice by the conjunctival route with the same dose and time intervals as in group C. Serological responses in agglutination, complement fixation and Rose Bengal tests were typical of those following standard vaccination with Strain B 19 in group B. Iu group C after the booster vaccination, there was a transient rise in titers which lasted about 3 months.

[Oxytetracycline treatment of cows with long-standing brucellosis].

Forty-five brucella infected cows were studied 15 to 30 days following abortion. Eighteen cows were slaughtered and autopsied for examination of Brucella in the organs and lymph nodes. The remaining 27 cows were treated during two and one-half months and then slaughtered after undergoing a treatment with 0, 1, 2 or 3 intraperitoneal injections of oxytetracycline dissolved in 100 ml of physiological saline.