The effect of bovine basic fibroblast growth factor on skin flap survival in rats.

Basic fibroblast growth factor (bFGF) was prepared from bovine pituitary glands and evaluated for its effect on the viability of pedicle skin flaps in rats. Pedicle flaps measuring 3 x 7.5 cm and based cephalad were created on the backs of animals.

Vitreous: an inhibitor of retinal extract-induced neovascularization.

One of the major problems in assessing neovascularization in mammalian experimental animal models is the immunologic response of the host to stimuli from nonautologous species. Hence, crude bovine vitreous and retinal extracts may produce a complex immune reaction when tested in the rabbit. To circumvent this problem, the chicken chorioallantoic membrane (CAM) assay is most appropriate.

Tumor angiogenic factor. Purification from the Walker 256 rat tumor.

We report here the purification of an angiogenic substance from the Walker 256 rat ascites tumor which is mitogenic for fetal bovine aortic endothelial cells in culture and which also stimulates new blood vessel growth in vivo. Purification was monitored in endothelial cell cultures by cell counting or by [3H]thymidine incorporation into DNA. Lyophilized crude tumor cell homogenate was extracted with absolute ethanol, and the extract was further purified by silica gel column chromatography.

Nucleoside requirements for the in vitro growth of bovine aortic endothelial cells.

The nutritional needs of cultured fetal bovine aortic endothelial cells were studied with regard to their nucleotide metabolism. When Medium 199 containing calf serum was supplemented with up to 5 microgram/ml of the deoxyribo- or ribonucleosides found in DNA or RNA, the rate of endothelial cell growth increased. The effect was entirely attributable to the pyrimidine nucleosides.

A corneal micropocket assay for angiogenesis in the rat eye.

A corneal micropocket assay for angiogenesis in the rat eye is described in detail. With our test system, a partially purified, low molecular-weight endothelial cell growth stimulatory factor isolated from the Walker 256 rat carcinosarcoma is demonstrated to have potent angiogenic activity in vivo. The advantages and applications of our rat corneal assay are discussed.

Angiogenic activity from bovine retina: partial purification and characterization.

Bovine retinas contain a factor that stimulates proliferation of aortic endothelial cells in culture as well as neovascularization on the chicken chorioallantoic membrane. The stimulatory activity has been partially purified from a balanced salt solution extract of bovine retinas. The stability of the activity to acid pH was utilized as the first step in purification.

Chemical mediators of intraocular neovascularization.

Intraocular neovascularization occurs in several ocular disorders. Evidence from clinical experimental observations suggests that the retina and the vitreous are potential sources of angiogenic stimulators and inhibitors which may play a role in the development of intraocular neonvascularization. We have demonstrated that extracts of adult retina are capable of stimulating vasoproliferation on the chick chorioallantoic membrane (CAM) and that extracts of adult vitreous inhibit this response.

The demonstration of angiogenic activity from ocular tissues. Preliminary report.

Human, bovine, and rabbit retinal extracts are shown to be capable of stimulating (1) proliferation and thymidine uptake of bovine vascular endothelial cells in cultures and (2) neovascularization of the chick chorioallantoic membrane (CAM). Extracts of skeletal muscle, heart, and liver lack similar stimulatory activity. Vitreous aspirates from patients with proliferative diabetic retinopathy, in a preliminary study, were able to stimulate vascular endothelial cell thymidine uptake.

Demonstration of vasoproliferative activity from mammalian retina.

Vasoproliferative activity has been demonstrated in extracts of retinas from human, bovine, and feline sources. These retinal extracts are capable of stimulating (a) proliferation and thymidine uptake of bovine vascular endothelial cells in culture and (b) neovascularization on the chick chorioallantoic membrane. Extracts of skeletal muscle, cardiac muscle, and liver lack similar stimulatory activity.

Subcellular localization of acetoacetate coenzyme A transferase in rat hepatomas.

Succinyl coenzyme A:acetoacetate coenzyme transferase (EC 2.8.3.

Growth stimulation of cultured endothelial cells by tumor cell homogenates.

Short-term cultures of endothelial cells from fetal bovine heart and aorta consistently display increased growth rates when crude tumor cell homogenates from the Walker 256 adenocarcinoma (ascites and solid forms as well as tumor cells from a suspension culture) are added to the culture media. The tumor-derived material produces growth stimulation in both sparse and confluient endothelial cell cultures. Homogenates of embryonic tissues and cultured cells show similar growth-promoting effects; corresponding material from various adult tissues is ineffective.

3-oxo acid coenzyme A-transferase in normal and diabetic rat muscle.

The amounts of succinyl-CoA--3-oxo acid CoA-transferase (EC 2.8.3.

Acetoacetate coenzyme A transferase activity in rat hepatomas.

The presence of succinyl-coenzyme A:acetoacetate CoA transferase (CoA transferase) (EC 2.8.3.

Comparative studies on 3-oxo acid coenzyme A transferase from various rat tissues.

1. Tissue activities, intracellular distribution as well as selected kinetic and molecular properties of succinyl-CoA-3-oxo acid CoA transferase (EC 2.8.