Silence after narratives by patients in psychodynamic psychotherapy: a conversation analytic study.

In psychotherapy, verbal communication is central to the therapeutic process. However, when patients remain silent, it can serve various functions, such as reflecting more deeply or hesitating to elaborate on a topic. This article uses conversation analysis to examine a specific context in which silence occurs: After a patient has concluded his/her narrative, both the therapist and the patient resist the turn allocation by the respective other, resulting in mutual silence.

UK Foot and Ankle Thromboembolism (UK-FATE).

Aims: Venous thromboembolism (VTE) is a potential complication of foot and ankle surgery. There is a lack of agreement on contributing risk factors and chemical prophylaxis requirements. The primary outcome of this study was to analyze the 90-day incidence of symptomatic VTE and VTE-related mortality in patients undergoing foot and ankle surgery and Achilles tendon (TA) rupture.

Preclinical evaluation in hamster model of the mRNA COVID-19 vaccine candidate AfriVac 2121 (Wuhan) produced under the WHO/MPP mRNA Technology Transfer Programme.

During the COVID-19 pandemic, access to vaccines in low- and middle-income countries was limited and delayed. To address these disparities, the mRNA Technology Transfer Programme, coordinated and led by the World Health Organization and the Medicines Patent Pool, was launched. A consortium has been set up in South Africa to develop a platform for manufacturing mRNA vaccines.

Controlled Covalent Conjugation of a Tuberculosis Subunit Antigen (ID93) to Liposome Improved Th1-Type Cytokine Recall Responses in Human Whole Blood.

Tuberculosis (TB) remains a foremost poverty-related disease with a high rate of mortality despite global immunization with Bacille Calmette-Guérin (BCG). Several adjuvanted recombinant proteins are in clinical development for TB to protect against the disease in infants and adults. Nevertheless, simple mixing of adjuvants with antigens may not be optimal for enhancing the immune response due to poor association.

A chemo-enzymatic oxidation cascade to activate C-H bonds with in situ generated HO.

Continuous low-level supply or in situ generation of hydrogen peroxide (HO) is essential for the stability of unspecific peroxygenases, which are deemed ideal biocatalysts for the selective activation of C-H bonds. To envisage potential large scale applications of combined catalytic systems the reactions need to be simple, efficient and produce minimal by-products. We show that gold-palladium nanoparticles supported on TiO or carbon have sufficient activity at ambient temperature and pressure to generate HO from H and O and supply the oxidant to the engineered unspecific heme-thiolate peroxygenase PaDa-I.

AVURT: aspirin versus placebo for the treatment of venous leg ulcers - a Phase II pilot randomised controlled trial.

Background: Venous leg ulcers (VLUs) are the most common cause of leg ulceration, affecting 1 in 100 adults. VLUs may take many months to heal (25% fail to heal). Estimated prevalence is between 1% and 3% of the elderly population.

Clinical examination and non-invasive screening tests in the diagnosis of peripheral artery disease in people with diabetes-related foot ulceration.

Aim: Peripheral artery disease is common in people with diabetes-related foot ulceration and is a risk factor for amputation. The best method for the detection or exclusion of peripheral artery disease is unknown. This study investigated the utility of clinical examination and non-invasive bedside tests in screening for peripheral artery disease in diabetes-related foot ulceration.

Effect of cell permeability and dehydrogenase expression on octane activation by CYP153A6-based whole cell Escherichia coli catalysts.

Background: The regeneration of cofactors and the supply of alkane substrate are key considerations for the biocatalytic activation of hydrocarbons by cytochrome P450s. This study focused on the biotransformation of n-octane to 1-octanol using resting Escherichia coli cells expressing the CYP153A6 operon, which includes the electron transport proteins ferredoxin and ferredoxin reductase. Glycerol dehydrogenase was co-expressed with the CYP153A6 operon to investigate the effects of boosting cofactor regeneration.

The influence of microbial physiology on biocatalyst activity and efficiency in the terminal hydroxylation of n-octane using Escherichia coli expressing the alkane hydroxylase, CYP153A6.

Background: Biocatalyst improvement through molecular and recombinant means should be complemented with efficient process design to facilitate process feasibility and improve process economics. This study focused on understanding the bioprocess limitations to identify factors that impact the expression of the terminal hydroxylase CYP153A6 and also influence the biocatalytic transformation of n-octane to 1-octanol using resting whole cells of recombinant E. coli expressing the CYP153A6 operon which includes the ferredoxin (Fdx) and the ferredoxin reductase (FdR).

Irinotecan(Campto R): efficacy as third/forth line therapy in advanced pancreatic cancer.

Following the concept that the actual survival of pancreatic cancer patients can only be significantly improved by sequential poly-chemotherapy (EOSPC) in order to add one or two further progression free-survival times (PFST), in addition to the potential antitumoral effects of a first- or second-line therapy we studied the therapeutic efficacy of a third- or fourth-line chemotherapy with irinotecan alone, or in combination with oxaliplatin and high dose 5-FU/FA respectively, in a pilot study in 17 patients. Follow-up was performed on the basis of clinical investigations, imaging methods and the course of tumor markers, mainly CT and CA 19-9. The overall response rate in these cases of third/fourth-line therapies was 1 PR, 4 MR, 6 SD in the imaging methods compared to 5 PR, 2 MR and 5 SD on the basis of the tumor marker courses in the serum.

Improvement of survival by efficacy orientated sequential polychemotherapy of exocrine pancreatic cancer.

Results of palliative chemotherapy in 104 patients suffering from exocrine pancreatic carcinomas are presented. First-line therapy included intraarterial approaches with gemcitabine + mitomycin-C and intravenous systemic treatments with gemcitabine, gemcitabine + mitomycin-C and oxaliplatin, respectively. In addition, it was the aim to improve survival by adding second- and third-line chemotherapies, mainly including high dose 5-FU/FA and irinotecan resp.

Analysis of K-ras mutations in pancreatic tissue after fine needle aspirates.

The majority of pancreatic carcinomas contain a mutation at codon 12 of the K-ras oncogene. We have analysed 87 samples from 76 patients who underwent surgery because of different pancreatic diseases to evaluate whether the detection of K-ras mutations may be helpful to discriminate between chronic inflammation and neoplastic growth. Mutation analysis was performed using a semi-nested PCR followed by a selective restriction enzyme digestion.

Regression of cetacean tattoo lesions concurrent with conversion of precipitin antibody against a poxvirus.

Tattoo lesions linked to the cetacean poxvirus of bottlenose dolphins regressed without treatment. Two types of regression were observed: (1) The tattoo lesions became raised and blanched, then disappeared along with sloughing skin. (2) When an incision was made through a tattoo lesion, the tattoo disappeared in a zone around the incision.

Weight-length relationships of wild-caught and captive Atlantic bottlenose dolphins.

Length and weight measurements were obtained on 144 Atlantic bottlenose dolphins (Tursiops truncatus). Data were compared for wild-caught dolphins (n = 124), dolphins in training (n = 34), and dolphins that had died in an emaciated condition (n = 15). The purpose of the study was to establish guidelines for use in estimating when a dolphin is over- or underweight.

Synthesis and phosphorylation of canine cardiac myosin in tissue-culture assessment of hypertrophying factors.

Canine cardiac myosin, which was synthesized in a 14-day tissue culture, based on L-[3H] leucine incorporation, was precipitated with goat gamma G antimyosin (cardiac-specific) and analyzed on dodecylsulfate gels. Incorporation of 32PO4 into myosin chains occurring in culture was the same as that obtained in vivo and appeared to be coupled with translation. Removal of 32PO4 from myosin heavy chains with base treatment indicated the presence of phosphoserine and phosphothreonine in canine cardiac myosin heavy chains.

Comparative analyses of the kinetics and subunits of myosins from canine skeletal muscle and cardiac tissue.

Two types of canine cardiac myosins, myosin from the free wall of the right ventricle and the free wall of the left ventricle, were compared with canine skeletal muscle myosin from the gastrocnemius. The Vmax values for the ATPase reaction catalyzed by myosin were significantly different among the three types of tissues. For K+-activated myosin the Vmax values in micromoles of Pi per mg per min were: right ventricle, 0.