Improved Risk Prediction of Acute Myocardial Infarction in Patients With Stable Coronary Artery Disease Using an Amino Acid-Assisted Model.

Patients with stable coronary artery disease (CAD) are at an increased risk of acute myocardial infarction (AMI), particularly among older individuals. Developing a reliable model to predict AMI occurrence in these patients holds the potential to expedite early diagnosis and intervention. This study is aimed at establishing a circulating amino acid-assisted model, incorporating amino acid profiles alongside clinical variables, to predict AMI risk.

ACSL4 and polyunsaturated lipids support metastatic extravasation and colonization.

Metastatic dissemination to distant organs demands that cancer cells possess high morphological and metabolic adaptability. However, contributions of the cellular lipidome to metastasis remain elusive. Here, we uncover a correlation between metastasis potential and ferroptosis susceptibility in multiple cancers.

Practical research on coal pillar retention in deep mining roadways.

To address the limitations of traditional coal pillar design methods in engineering practice for deep retreat mining roadways, this research focuses on the retreat mining face at the Pinggang Coal Mine in Heilongjiang Province. Combining mechanical experiments, theoretical calculations, numerical simulations, and field tests, this study calculates the ultimate equilibrium size of coal pillars. It also analyses the stress transfer and surrounding rock plastic evolution characteristics under different coal pillar sizes in deep retreat mining faces and carries out a field engineering application.

ETV4‑mediated transcriptional activation of SLC12A5 exacerbates ferroptosis resistance and glucose metabolism reprogramming in breast cancer cells.

Solute carrier family 12 member 5 (SLC12A5) is an oncogene in numerous types of cancer, however its function in breast cancer (BC) remains elusive. ETS translocation variant 4 (ETV4) promotes BC. Therefore, the present study aimed to elucidate the role of SLC12A5 in ferroptosis and glucose metabolism in BC cells as well as to understand the underlying mechanism.

Five cases of pulmonary nodules diagnosed at surgery and by pathology in immunocompetent patients, with a literature review.

A pulmonary nodule is a rare subtype of chronic pulmonary aspergillosis. The diagnosis is difficult and is histological. There are only a few reports on such cases.

Fast surface signal extraction method for photon point clouds with strong background noise without prior altitude information.

It is extremely challenging to rapidly and accurately extract target echo photon signals from massive photon point clouds with strong background noise without any prior geographic information. Herein, we propose a fast surface detection method realized by combining the improved density-dimension algorithm (DDA) and Kalman filtering (KF), termed the DDA-KF algorithm, for photon signals with a high background noise rate (BNR) to improve the extraction of surface photon signals from spacecraft platforms. The results showed that the algorithm exhibited good adaptability to strong background noise and terrain slope variations, and had real-time processing capabilities for massive photon point clouds in large-scale detection range without prior altitude information of target.

Regulation of RAS palmitoyltransferases by accessory proteins and palmitoylation.

Palmitoylation of cysteine residues at the C-terminal hypervariable regions in human HRAS and NRAS, which is necessary for RAS signaling, is catalyzed by the acyltransferase DHHC9 in complex with its accessory protein GCP16. The molecular basis for the acyltransferase activity and the regulation of DHHC9 by GCP16 is not clear. Here we report the cryo-electron microscopy structures of the human DHHC9-GCP16 complex and its yeast counterpart-the Erf2-Erf4 complex, demonstrating that GCP16 and Erf4 are not directly involved in the catalytic process but stabilize the architecture of DHHC9 and Erf2, respectively.

Stratifying Ferroptosis Sensitivity in Cells and Tissues with PALP.

Ferroptosis is emerging as a promising strategy for suppressing multiple types of human cancers. Rapid and accurate assessment of the relative sensitivity to ferroptosis in biological samples will accelerate the development of ferroptosis-targeted therapies. We previously demonstrated that photochemical activation of membrane lipid peroxidation (PALP) that uses high-power lasers to induce localized polyunsaturated fatty acyl (PUFA)-lipid peroxidation can efficiently report ferroptosis sensitivity in live cells and tissues in situ.

A microdots array-based fluoremetric assay with superwettability profile for simultaneous and separate analysis of iron and copper in red wine.

A microdots array-based fluoremetric method with superwettability profile has been developed for the simultaneous and separate detection of Fe and Cu ions in red wine samples. A wettable micropores array was initially designed with high density by using polyacrylic acid (PAA) and hexadecyltrimethoxysilane (HDS), followed by the NaOH etching route. Zinc metal organic frameworks (Zn-MOFs) were fabricated as the fluorescent probes to be immobilized into the micropores array to obtain the fluoremetric microdots array platform.

Fabrication and Calibration of Pt-Rh10/Pt Thin-Film Thermocouple.

Aiming at the dynamic testing of the ignition temperature of micro-initiating explosives, a novel Pt-Rh10/Pt thin-film thermocouple was designed in this paper. The author carried out the preparation of the thermocouple by using a screen printing process on an AlO ceramic substrate. The formed thermocouple was made of Pt-Rh10 wire and Pt wire as compensation wires, with a size of ≤ 1 mm and a thickness of about 6 μm.

Single Cell Transcriptome Analysis of Peripheral Blood Mononuclear Cells in Freshly Isolated versus Stored Blood Samples.

Background: Peripheral blood mononuclear cells (PBMCs) are widely used as a model in the study of different human diseases. There is often a time delay from blood collection to PBMC isolation during the sampling process, which can result in an experimental bias, particularly when performing single cell RNA-seq (scRNAseq) studies.

Methods: This study examined the impact of different time periods from blood draw to PBMC isolation on the subsequent transcriptome profiling of different cell types in PBMCs by scRNAseq using the 10X Chromium Single Cell Gene Expression assay.

Enhanced Photoluminescence of GdAlGaO: Cr by Energy Transfers from Co-Doped Dy.

LEDs for plant lighting have attracted wide attention and phosphors with good stability and deep-red emission are urgently needed. Novel Cr3+ and Dy3+ co-doped Gd3Al4GaO12 garnet (GAGG) phosphors were successfully prepared through a conventional solid-state reaction. Using blue LEDs, a broadband deep-red emission at 650−850 nm was obtained due to the Cr3+ 4T2 → 4A2 transition.

Covalent organic framework-loaded silver nanoparticles as robust mimetic oxidase for highly sensitive and selective colorimetric detection of mercury in blood.

Covalent organic frameworks (COFs) were fabricated with a hierarchical flower-like hollow structure, possessing a large specific surface area, high porosity, and excellent environmental stability. growth of noble silver nanoparticles (AgNPs) onto COFs was conducted yielding COF-Ag nanozymes. The structural advantages of COFs can ensure the uniform dispersion and effective size control of AgNPs.

Comparison of outcomes of idiopathic macular holes treated by vitrectomy with air or silicone oil tamponade based on the hole size.

Aim: To compare the efficacy of vitrectomy combined with air or silicone oil in the treatment of idiopathic macular hole (IMH).

Methods: According to the results of high-definition optical coherence tomography (HD-OCT), 75 cases (75 eyes) of IMH in stage II-IV (Gass stage) in the General Hospital of Chinese PLA from January 2017 to December 2019 were collected for this retrospective study. The best corrected visual acuity (BCVA) and minimum diameter of IMH (MMHD) were measured.

Treatment of Massive Suprachoroidal Hemorrhage by Drainage Surgery with Vitrectomy: Experience of a Single Center.

Purpose: The present study assessed the treatment of and risk factors for suprachoroidal hemorrhage (SCH) and reported the outcomes of drainage surgery with vitrectomy in a consecutive series of patients.

Methods: Retrospective case series were carried out to investigate the clinical data of 12 eyes from 12 patients who underwent suprachoroidal hemorrhage drainage with vitrectomy surgery over a 10-year period (from 2010 to 2020). The records of these patients were analyzed, including ophthalmologic examination, ophthalmologic ultrasonography, surgical procedures, and outcome measurements.

Recognition and Classification of Ship Images Based on SMS-PCNN Model.

In the field of ship image recognition and classification, traditional algorithms lack attention to the differences between the grain of ship images. The differences in the hull structure of different categories of ships are reflected in the coarse-grain, whereas the differences in the ship equipment and superstructures of different ships of the same category are reflected in the fine-grain. To extract the ship features of different scales, the multi-scale paralleling CNN oriented on ships images (SMS-PCNN) model is proposed in this paper.

Identification of Novel Loci Shared by Juvenile Idiopathic Arthritis Subtypes Through Integrative Genetic Analysis.

Objective: Juvenile idiopathic arthritis (JIA) is the most common chronic immune-mediated joint disease among children and encompasses a heterogeneous group of immune-mediated joint disorders classified into 7 subtypes according to clinical presentation. However, phenotype overlap and biologic evidence suggest a shared mechanistic basis between subtypes. This study was undertaken to systematically investigate shared genetic underpinnings of JIA subtypes.

Stratifying ferroptosis sensitivity in cells and mouse tissues by photochemical activation of lipid peroxidation and fluorescent imaging.

Ferroptosis is a non-apoptotic iron-dependent cell death. Here we present a protocol for stratifying ferroptosis sensitivity in cells and mouse tissues. This protocol uses photochemical activation of lipid peroxidation (PALP) coupled with fluorescent imaging to assess the relative sensitivity to ferroptosis.

PALP: A rapid imaging technique for stratifying ferroptosis sensitivity in normal and tumor tissues in situ.

Ferroptosis is an emerging cancer suppression strategy. However, how to select cancer patients for treating with ferroptosis inducers remains challenging. Here, we develop photochemical activation of membrane lipid peroxidation (PALP), which uses targeted lasers to induce localized polyunsaturated fatty acyl (PUFA)-lipid peroxidation for reporting ferroptosis sensitivity in cells and tissues.

Prediction of dMRI signals with neural architecture search.

Background: There is growing interest in the neuroscience community in estimating and mapping microscopic properties of brain tissue non-invasively using magnetic resonance measurements. Machine learning methods are actively investigated to predict the signals measured in diffusion magnetic resonance imaging (dMRI).

New Method: We applied the neural architecture search (NAS) to train a recurrent neural network to generate a multilayer perceptron to predict the dMRI data of unknown signals based on the different acquisition parameters and training data.

Clinical Study of Stellate Ganglion Block Combined with General Anesthesia on Hemodynamics, Cognitive Function, and Gastrointestinal Function in Elderly Patients Undergoing Partial Hepatectomy.

Partial hepatectomy under general anesthesia is prone to hemodynamic alterations, and stress reactions are the main contributing factors to postoperative cognitive function in elderly partial hepatectomy patients. Postoperative cognitive dysfunction increases the incidence of postoperative complications and long-term morbidity and mortality in elderly patients. With the increasing trend of aging population and the gradual increase of elderly people undergoing surgical treatment, it is especially important to study the corresponding prevention and treatment measures.

Construction and Validation of a Lung Cancer Diagnostic Model Based on 6-Gene Methylation Frequency in Blood, Clinical Features, and Serum Tumor Markers.

Lung cancer has a high mortality rate. Promoting early diagnosis and screening of lung cancer is the most effective way to enhance the survival rate of lung cancer patients. Through computer technology, a comprehensive evaluation of genetic testing results and basic clinical information of lung cancer patients could effectively diagnose early lung cancer and indicate cancer risks.

On the generalizability of diffusion MRI signal representations across acquisition parameters, sequences and tissue types: Chronicles of the MEMENTO challenge.

Diffusion MRI (dMRI) has become an invaluable tool to assess the microstructural organization of brain tissue. Depending on the specific acquisition settings, the dMRI signal encodes specific properties of the underlying diffusion process. In the last two decades, several signal representations have been proposed to fit the dMRI signal and decode such properties.

Untargeted metabolomics and lipidomics uncovering the cardioprotective effects of Huanglian Jiedu Decoction on pathological cardiac hypertrophy and remodeling.

Ethnopharmacological Relevance: As a classic herbal prescription, Huanglian Jiedu Decoction (HLJDD) exhibits positive effects against cardiac dysfunction. However, its cardioprotective effects and potential mechanism(s) of action still need to be systematically investigated.

Aim Of The Study: This study aimed to reveal the underlying therapeutic mechanism of HLJDD on transverse aortic constriction (TAC)-induced pathological cardiac hypertrophy and remodeling.