Publications by authors named "Fengwu Qiu"
Article Synopsis
- Cervical cancer is a major health issue for women, ranking fourth in incidence and mortality, with the RNA-binding protein YTHDF2 playing a role in its progression through unclear mechanisms.
- * Researchers conducted various assays to study the effects of knocking down YTHDF2 on cervical cancer cells, finding that it influences tumor stemness and apoptosis.
- * Data analysis suggested that YTHDF2 regulates GLI2, leading to increased JNK activity and endoplasmic reticulum stress, which ultimately suppresses cervical cancer cell growth and highlights YTHDF2 as a potential therapeutic target.
[Identification and the Related Molecular Mechanism of Allele].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
October 2023
Objective: To investigate the identification and molecular biological mechanism of a case of allele.
Methods: The ABO blood groups of the proband and his nine family members were analyzed serologically and DNA sequencing was used to accurately determine the genotypes of these ten specimens. The cartoon models of local active center of enzymes of the GTA,GTB and the GTB mutant were constructed to explore the possible molecular mechanism leading to abnormal enzyme-catalyzed A antigen synthesis.
Article Synopsis
- - The hepatitis B virus X protein (HBx) is linked to the development of liver cancer (hepatocellular carcinoma) by inhibiting the expression of microRNA-187-5p (miR-187-5p).
- - Research shows that increasing miR-187-5p levels can reduce cancer cell growth, spread, and invasion, and that its downregulation may occur via the E2F1/FoxP3 pathway influenced by HBx.
- - Ultimately, the study suggests that HBx promotes liver cancer cell activity through the E2F1/FoxP3/miR-187 pathway, highlighting potential targets for new treatments.
[Serological Characteristics and Molecular Biological Mechanism of AEL.02 Subtype].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
October 2022
Article Synopsis
- Objective is to analyze the serological and molecular characteristics of an a blood subtype specimen.
- Methods include blood type identification through serological tests and PCR sequencing for ABO genotyping, followed by constructing a 3D molecular model to evaluate the stability of the GTA mutant.
- Results show discrepancies in serological typing, identified mutations affecting protein stability, and indicate that serological characteristics are not sufficient alone to determine the a subtype, suggesting it may be linked to the GTA mutant affecting enzyme stability.
Characterizing the long-term nanometer-scale interactions between lysosomes and mitochondria in live cells is essential for understanding their functions but remains challenging due to limitations of the existing fluorescent probes. Here, we develop cell-permeable organic fluorescent probes for lysosomes with excellent specificity and high photostability. We also use an existing Atto 647N dye with high brightness and excellent photostability to achieve specific labeling of mitochondria in live cells.
View Article and Find Full Text PDFAssociation of HLA alleles (A, B, DRB1) and HIV-1 infection in the Han population of Hubei, China.
J Huazhong Univ Sci Technolog Med Sci
February 2017
The HIV susceptibility and resistance alleles in the HLA genes were determined by investigating the distribution characteristics of the HLA alleles (A, B, and DRB1) in HIV-infected individuals of the Han population in Hubei, and by comparing these alleles with HIV-negative individuals from the same area. A cohort of 424 HIV-1 infected individuals were chosen as study subjects, and 836 HIV-negative healthy subjects from the same area served as the control population. HLA-A, B, and DRB1 allele typing was performed using polymerase chain reaction-sequence-specific oligonucleotide probes (PCR-SSOP) and polymerase chain reaction-sequencing based typing (PCR-SBT) techniques.
View Article and Find Full Text PDFSingle-molecule localization microscopy (SMLM) achieves super-resolution imaging beyond the diffraction limit but critically relies on the use of photo-modulatable fluorescent probes. Here we report a general strategy for constructing cell-permeable photo-modulatable organic fluorescent probes for live-cell SMLM by exploiting the remarkable cytosolic delivery ability of a cell-penetrating peptide (rR)3R2. We develop photo-modulatable organic fluorescent probes consisting of a (rR)3R2 peptide coupled to a cell-impermeable organic fluorophore and a recognition unit.
View Article and Find Full Text PDFDNA therapy for cancer requires efficient, selective and safe DNA delivery systems. Compared with other non-viral methods such as lipid or polymer-based DNA delivery vectors, peptide-based DNA delivery systems are biocompatible and biodegradable, which leads to lower immunogenicity and lower toxicity. Moreover, peptide vectors are easier to produce and their compositions easier to control because solid-phase peptide synthesis has been extensively developed.
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